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| 1 | EB病毒感染实验室诊断及临床应用专家共识显示文摘1前言
EB病毒(Epstein.Barrvirus,EBV)为疱疹病毒科,疱疹病毒IV型,是一种嗜人类淋巴细胞的疱疹病毒。EBV是双链DNA病毒,基因组长约172kb,在病毒颗粒中呈线性分子,进入受感染细胞后,其DNA发生环化并能自我复制。淋巴细胞中潜伏感染的EBV可表达2种不翻译成蛋白质的RNA(即EBV—encoded RNAs,EBERs),包括EBER1和EBER2,6种核抗原(EBNA1、EBNA2、EBNA3A、 | | 2018 | 中华实验和临床病毒学杂志2018，32，1： | 97 |
| 2 | Function of lncRNAs and approaches to lncRNA-protein interactions显示文摘Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs have been found to play important roles in normal cell physiological activities,and participate in the development of varieties of tumors and other diseases.Previously,we have only been able to determine the function of lncRNAs through multiple mechanisms,including genetic imprinting,chromatin remodeling,splicing regulation,mRNA decay,and translational regulation.Application of technological advances to research into the function of lncRNAs is extremely important.The major tools for exploring lncRNAs include microarrays,RNA sequencing(RNA-seq),Northern blotting,real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),RNA interference(RNAi),RNA-binding protein immunoprecipitation(RIP),chromatin isolation by RNA purification(ChIRP),crosslinking-immunopurification(CLIP),and bioinformatic prediction.In this review,we highlight the functions of lncRNAs,and advanced methods to research lncRNA-protein interactions. | ZHU JuanJuan FU HanJiang WU YongGe ZHENG XiaoFei | 2013 | Science China(Life Sciences)2013，56，10： | 54 |
| 3 | Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System显示文摘 | Mugui Wang Yanfei Mao Yuming Lu Xiaoping Tao Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 56 |
| 4 | Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication显示文摘AIM: To screen and investigate the effective g RNAs against hepatitis B virus(HBV) of genotypes A-D.METHODS: A total of 15 g RNAs against HBV of genotypes A-D were designed. Eleven combinations of two above g RNAs(dual-g RNAs) covering the regulatory region of HBV were chosen. The efficiency of each g RNA and 11 dual-g RNAs on the suppression of HBV(genotypes A-D) replication was examined by the measurement of HBV surface antigen(HBs Ag) or e antigen(HBe Ag) in the culture supernatant. The destruction of HBV-expressing vector was examined in Hu H7 cells co-transfected with dual-g RNAs and HBVexpressing vector using polymerase chain reaction(PCR) and sequencing method, and the destruction of ccc DNAwas examined in Hep AD38 cells using KCl precipitation, plasmid-safe ATP-dependent DNase(PSAD) digestion, rolling circle amplification and quantitative PCR combined method. The cytotoxicity of these g RNAs was assessed by a mitochondrial tetrazolium assay.RESULTS: All of g RNAs could significantly reduce HBs Ag or HBe Ag production in the culture supernatant, which was dependent on the region in which g RNA against. All of dual g RNAs could efficiently suppress HBs Ag and/or HBe Ag production for HBV of genotypes A-D, and the efficacy of dual g RNAs in suppressing HBs Ag and/or HBe Ag production was significantly increased when compared to the single g RNA used alone. Furthermore, by PCR direct sequencing we confirmed that these dual g RNAs could specifically destroy HBV expressing template by removing the fragment between the cleavage sites of the two used g RNAs. Most importantly, g RNA-5 and g RNA-12 combination not only could efficiently suppressing HBs Ag and/or HBe Ag production, but also destroy the ccc DNA reservoirs in Hep AD38 cells.CONCLUSION: These results suggested that CRISPR/Cas9 system could efficiently destroy HBV expressing templates(genotypes A-D) without apparent cytotoxicity. It may be a potential approach for eradication of persistent HBV ccc DNA in chronic HBV infection patients. | Jie Wang Zhong-Wei Xu Shuang Liu Rui-Yang Zhang Shan-Long Ding Xiao-Meng Xie Lu Long Xiang-Mei Chen Hui Zhuang Feng-Min Lu | 2015 | World Journal of Gastroenterology2015，21，32： | 29 |
| 5 | Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials显示文摘Objective:To identify differentially expressed long non-coding RNAs(lncRNAs) involved in the metastasis of epithelial ovarian cancer.Methods:An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and SKOV3.ip1 cell lines.Total RNA was then extracted,and microarray analysis was performed.Moreover,nine lncRNAs were selected for validation using RT-qPCR.Results:Compared with the SKOV3 cells,the SKOV3.ip1 cells significantly improved in the in vitro invasive activity.Of the 4,956 lncRNAs detected in the microarray,583 and 578 lncRNAs were upregulated and downregulated,respectively,in SKOV3.ip1 cells,compared with the parental SKOV3 cells.Seven of the analyzed lncRNAs(MALAT1,H19,UCA1,CCAT1,LOC645249,LOC100128881,and LOC100292680) confirmed the deregulation found by microarray analysis.Conclusion:LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials.This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis.Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis. | Shi-Ping Liu Jia-Xin Yang Dong-Yan Cao Keng Shen | 2013 | Cancer Biology & Medicine2013，10，3： | 27 |
| 6 | 通督调神针灸预处理对脑缺血再灌注大鼠miRNA664及MMP9调控机制的研究显示文摘目的探讨通督调神针灸预处理对脑缺血再灌注大鼠神经行为功能和脑水含量的影响及mi RNA664、MMP9表达的调控作用机制。方法将130只Wistar大鼠随机分为A组(电针预处理组)30只、B组(艾灸预处理组)30只、C组(阿司匹林预处理组)30只、D组(模型组)30只和E组(空白对照组)10只。A组进行电针治疗;B组用清艾条悬灸;C组用阿司匹林10 mg/kg灌胃。治疗7 d后各组进行脑缺血再灌注模型制作,于再灌注后24 h,观察大鼠神经行为功能和脑水含量以及皮质区相关mi RNA、MMP9的表达。结果 A组、B组、C组和D组神经行为学评分与E组比较,差异均具有统计学意义(P<0.01)。A组、B组神经行为学评分与C组比较,差异均具有统计学意义(P<0.01)。A组神经行为学评分与B组比较,差异具有统计学意义(P<0.01)。A组、B组、C组和D组脑水含量、mi RNA664相对表达量及MMP9相对表达量与E组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组、B组和C组脑水含量、mi RNA664相对表达量及MMP9相对表达量与D组比较,差异均具有统计学意义(P<0.01)。A组和B组脑水含量、mi RNA664相对表达量及MMP9相对表达量与C组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组脑水含量、mi RNA664相对表达量及MMP9相对表达量与B组比较,差异具有统计学意义(P<0.01,P<0.05)。结论通督调神针灸预处理可有效降低脑缺血再灌注大鼠的神经行为学评分和脑水含量,并通过调控mi RNA664的表达降低MMP9相对表达量。通督调神针灸预处理脑保护机制之一可能通过调控mi RNA664的表达降低MMP9相对表达量,诱导脑缺血耐受,减轻脑水肿。 | 郑仕平 韩为 储浩然 王颖 张玲 张国庆 朱玲玲 | 2016 | 上海针灸杂志2016，35，1： | 24 |
| 7 | Emerging potential of exosomes for treatment of traumatic brain injury显示文摘Traumatic brain injury(TBI) is one of the major causes of death and disability worldwide.No effective treatment has been identified from clinical trials.Compelling evidence exists that treatment with mesenchymal stem cells(MSCs) exerts a substantial therapeutic effect after experimental brain injury.In addition to their soluble factors,therapeutic effects of MSCs may be attributed to their generation and release of exosomes.Exosomes are endosomal origin small-membrane nano-sized vesicles generated by almost all cell types.Exosomes play a pivotal role in intercellular communication.Intravenous delivery of MSC-derived exosomes improves functional recovery and promotes neuroplasticity in rats after TBI.Therapeutic effects of exosomes derive from the exosome content,especially micro RNAs(mi RNAs).mi RNAs are small non-coding regulatory RNAs and play an important role in posttranscriptional regulation of genes.Compared with their parent cells,exosomes are more stable and can cross the blood-brain barrier.They have reduced the safety risks inherent in administering viable cells such as the risk of occlusion in microvasculature or unregulated growth of transplanted cells.Developing a cell-free exosome-based therapy may open up a novel approach to enhancing multifaceted aspects of neuroplasticity and to amplifying neurological recovery,potentially for a variety of neural injuries and neurodegenerative diseases.This review discusses the most recent knowledge of exosome therapies for TBI,their associated challenges and opportunities. | Ye Xiong Asim Mahmood Michael Chopp | 2017 | Neural Regeneration Research2017，12，1： | 22 |
| 8 | Circulating micro RNAs and long non-coding RNAs in gastric cancer diagnosis:An update and review显示文摘Gastric cancer(GC) is the fourth most common cancer and the third leading cause of cancer mortality worldwide. Micro RNAs(mi RNAs) and long non-coding RNAs(lnc RNAs) are the most popular non-coding RNAs in cancer research. To date,the roles of mi RNAs and lnc RNAs have been extensively studied in GC,suggesting that mi RNAs and lnc RNAs represent a vital component of tumor biology. Furthermore,circulating mi RNAs and lnc RNAs are found to be dysregulated in patients with GC compared with healthy individuals. Circulating mi RNAs and lnc RNAs may function as promising biomarkers to improve the early detection of GC. Multiple possibilities for mi RNA secretion have been elucidated,including active secretion by microvesicles,exosomes,apoptotic bodies,highdensity lipoproteins and protein complexes as well as passive leakage from cells. However,the mechanism underlying lnc RNA secretion and the functions of circulating mi RNAs and lnc RNAs have not been fully illuminated. Concurrently,to standardize results of global investigations of circulating mi RNAs and lnc RNAs biomarker studies,several recommendations for preanalytic considerations are put forward. In this review,we summarize the known circulating mi RNAs and lnc RNAs for GC diagnosis. The possible mechanism of mi RNA and lnc RNA secretion as well as methodologies for identification of circulating mi RNAs and lnc RNAs are also discussed. The topics covered here highlight new insights into GC diagnosis and screening. | Ya-Kai Huang Jian-Chun Yu | 2015 | World Journal of Gastroenterology2015，21，34： | 22 |
| 9 | Noncoding RNAs in gastric cancer: Research progress and prospects显示文摘Noncoding RNAs(nc RNAs) have attracted much attention in cancer research field. They are involved in cellular development, proliferation, differentiation and apoptosis. The dysregulation of nc RNAs has been reported in tumor initiation, progression, invasion and metastasis in various cancers, including gastric cancer(GC). In the past few years, an accumulating body of evidence has deepened our understanding of nc RNAs, and several emerging nc RNAs have been identified, such as PIWI-interacting RNAs(pi RNAs) and circular RNAs(circ RNAs). The competing endogenous RNA(ce RNA) networks include m RNAs, micro RNAs, long nc RNAs(lnc RNAs) and circ RNAs, which play critical roles in the tumorigenesis of GC. This review summarizes the recent hotspots of nc RNAs involved in GC pathobiology and their potential applications in GC. Finally, we briefly discuss the advances in the ce RNA network in GC. | Meng Zhang Xiang Du | 2016 | World Journal of Gastroenterology2016，22，29： | 20 |
| 10 | Diagnostic and prognostic potential of tissue and circulating long non-coding RNAs in colorectal tumors显示文摘Long non-coding RNAs(lncRNAs)are members of the non-protein coding RNA family longer than 200 nucleotides.They participate in the regulation of gene and protein expression influencing apoptosis,cell proliferation and immune responses,thereby playing a critical role in the development and progression of various cancers,including colorectal cancer(CRC).As CRC is one of the most frequently diagnosed malignancies worldwide with high mortality,its screening and early detection are crucial,so the identification of disease-specific biomarkers is necessary.LncRNAs are promising candidates as they are involved in carcinogenesis,and certain lncRNAs(e.g.,CCAT1,CRNDE,CRCAL1-4)show altered expression in adenomas,making them potential early diagnostic markers.In addition to being useful as tissue-specific markers,analysis of circulating lncRNAs(e.g.,CCAT1,CCAT2,BLACAT1,CRNDE,NEAT1,UCA1)in peripheral blood offers the possibility to establish minimally invasive,liquid biopsy-based diagnostic tests.This review article aims to describe the origin,structure,and functions of lncRNAs and to discuss their contribution to CRC development.Moreover,our purpose is to summarise lncRNAs showing altered expression levels during tumor formation in both colon tissue and plasma/serum samples and to demonstrate their clinical implications as diagnostic or prognostic biomarkers for CRC. | Orsolya Galamb Barbara K Barták Alexandra Kalmár Zsófia B Nagy Krisztina A Szigeti Zsolt Tulassay Peter Igaz Béla Molnár | 2019 | World Journal of Gastroenterology2019，25，34： | 18 |
| 11 | Roles of the canonical myomiRs mi R-1,-133 and-206 in cell development and disease显示文摘Micro RNAs are small non-coding RNAs that participate in different biological processes, providing subtle combinational regulation of cellular pathways, often by regulating components of signalling pathways. Aberrant expression of mi RNAs is an important factor in the development and progression of disease. The canonical myomi Rs(mi R-1,-133 and-206) are central to the development and health of mammalian skeletal and cardiac muscles, but new findings show they have regulatory roles in the development of other mammalian non-muscle tissues, including nerve, brain structures, adipose and some specialised immunological cells. Moreover, the deregulation of myomi R expression is associated with a variety of different cancers, where typically they have tumor suppressor functions, although examples of an oncogenic role illustrate their diverse function in different cell environments. This review examines the involvement of the related myomi Rs at the crossroads between cell development/tissue regeneration/tissue inflammation responses, and cancer development. | Keith Richard Mitchelson Wen-Yan Qin | 2015 | World Journal of Biological Chemistry2015，6，3： | 18 |
| 12 | The RNA Modification N^6-methyladenosine and Its Implications in Human Disease显示文摘Impaired gene regulation lies at the heart of many disorders,including developmental diseases and cancer.Furthermore,the molecular pathways that control gene expression are often the target of cellular parasites,such as viruses.Gene expression is controlled through multiple mechanisms that are coordinated to ensure the proper and timely expression of each gene.Many of these mechanisms target the life cycle of the RNA molecule,from transcription to translation.Recently,another layer of regulation at the RNA level involving RNA modifications has gained renewed interest of the scientific community.The discovery that N^6-methyladenosine(m^6A),a modification present in m RNAs and long noncoding RNAs,can be removed by the activity of RNA demethylases,launched the field of epitranscriptomics;the study of how RNA function is regulated through the addition or removal of post-transcriptional modifications,similar to strategies used to regulate gene expression at the DNA and protein level.The abundance of RNA post-transcriptional modifications is determined by the activity of writer complexes(methylase) and eraser(RNA demethylase) proteins.Subsequently,the effects of RNA modifications materialize as changes in RNA structure and/or modulation of interactions between the modified RNA and RNA binding proteins or regulatory RNAs.Disruption of these pathways impairs gene expression and cellular function.This review focuses on the links between the RNA modification m^6A and its implications in human diseases. | Pedro J.Batista | 2017 | Genomics, Proteomics & Bioinformatics2017，15，3： | 17 |
| 13 | Circulating micro RNAs as diagnostic and prognostic tools for hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC) is an aggressive malignancy and the second leading cause of cancerrelated deaths worldwide. Conventional biomarkers exhibit poor performance in the surveillance,diagnosis,and prognosis of HCC. Micro RNAs(mi RNAs) are a class of evolutionarily conserved small non-coding RNAs that are involved in the regulation of gene expression and protein translation,and they play critical roles in cell growth,differentiation,and the development of various types of cancers,including HCC. Recent evidence revealed the role of mi RNAs as potential novel and ideal biomarkers for HCC. mi RNAs are released to extracellular spaces,and they are extremely stable in bodily fluids,including serum or plasma,where they are packaged into various microparticles or associated with RNA-binding proteins. Numerous studies have demonstrated that circulating mi RNAs have potential applications as minimally invasive biomarkers for HCC diagnosis and prognosis. The present review highlights current understanding of mi RNA biogenesis and the origins and types of circulating mi RNAs. We summarize recent progress in the use of circulating mi RNAs as diagnostic and prognostic biomarkers for HCC. We also discuss the challenges and perspectives of the clinical utility of circulating mi RNAs in HCC. | Yu-Cheng Zhang Zhuo Xu Tian-Fu Zhang Ya-Li Wang | 2015 | World Journal of Gastroenterology2015，21，34： | 17 |
| 14 | miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis显示文摘AIM:To profile expression of micro RNAs(mi RNAs)in gastric cancer cells and investigate the effect of mi R-374b-5p on gastric cancer cell invasion and metastasis.METHODS:An mi RNA microarray assay was performed to identify mi RNAs differentially expressed in gastric cancer cell lines(MGC-803 and SGC-7901)compared with a normal gastric epithelial cell line.Upregulation of mi R-374b-5p was newly identified and confirmed via quantitative real-time reverse transcriptionPCR(q RT-PCR).MGC-803 cells were transfected with a synthesized anti-mi R-374b-5p sequence or a control vector using Lipofectamine reagent,or treated with transfection reagent alone or phosphate-buffered saline as controls.Rate of transfection was verified after 48 h by q RT-PCR.Cells were then subjected to transwell migration,wound scratch and cell counting kit-8 assays.A bioinformatic analysis to identify mi R-374b-5p target genes was performed using mi Randa,Pic Tar and Target Scan software.A dual luciferase reporter assay was performed to evaluate the influence of mi R-374b-5p on target gene activation,and q RT-PCR and Western blot were used to evaluate the levels of target m RNA and protein following transfection with mi R-374b-5p antisense oligonucleotides.RESULTS:The microarray profiling revealed downregulation of 14(fold change<0.667;P<0.05)and upregulation of 12(fold change>1.50;P<0.05)mi RNAs in MGC-803 and SGC-7901 cells compared with GES-1 controls.The upregulation of mi R-374b-5p(fold change=1.75 and 1.64 in MGC-803 and SGC-7901,respectively;P<0.05)was confirmed by q RT-PCR.Compared with the control groups,the restoration of mi R-374b-5p expression with anti-mi R-374b-5p significantly suppressed the metastasis,invasion and proliferation of MGC-803 cells.The bioinformatic analysis predicted that the 3’untranslated region(UTR)of reversion-inducing cysteine-rich protein with Kazal motif(RECK)contains three mi R-374b-5p target sequences.RECK was verified as a target gene in a dual luciferase reporter assay showing that activation of RECK 3’UTR-pmir GLO was increased by co-transfection with mi R-374b-5p.Finally,transfection of mi R-374b-5p antisense oligonucleotides increased m RNA and protein levels of RECK in MGC-803cells(P<0.05).CONCLUSION:These findings indicate that upregulation of mi R-374b-5p contributes to gastric cancer cell metastasis and invasion through inhibition of RECK expression. | Juan Xie Zhi-Hui Tan Xia Tang Ming-Shu Mo Yan-Ping Liu Run-Liang Gan Yi Li Li Zhang Guo-Qing Li | 2014 | World Journal of Gastroenterology2014，20，46： | 15 |
| 15 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 16 | 黄瓜生长素反应因子(ARF)家族鉴定及表达特异性分析显示文摘【目的】鉴定黄瓜生长素反应因子(ARF),预测small RNAs并验证ARF与small RNAs和生长素的关系;分析ARF在种子萌发过程中的表达模式,推断ARF在黄瓜单性结实和种子萌发过程中是否起到关键作用。【方法】利用拟南芥和水稻ARF蛋白质序列检索黄瓜基因组数据库,对检索到的黄瓜ARF家族进行结构分析和small RNAs预测;将预测到的gma-MIR160o precursur构建到pCAMBIA2301植物表达载体上,利用农杆菌介导法将其导入到单性结实黄瓜品种中,并对PCR检测为阳性的转基因植株进行RT-PCR验证;利用real-time RT-PCR方法分析ARF家族成员在生长素诱导后、开花时期及种子萌发阶段的表达模式。【结果】通过与拟南芥和水稻ARF蛋白序列的比对,共得到18个黄瓜ARF蛋白质序列。将其连同拟南芥和水稻的ARF蛋白质序列共分为4大类。从结构上看,外显子数目2-18不等,但同一类之间基因结构较为相似。进化树分析显示,18个基因之间的相似性不高;18个黄瓜ARF基因均有相对应的small RNA。其中,Csa010564、Csa011935、Csa015176、Csa020560和Csa022361可能都是miR160的靶基因。转基因试验进一步说明Csa010564、Csa011935和Csa015176在表达量上出现下降趋势,而Csa020560和Csa022361的表达量与对照相比略有上升,说明Csa010564、Csa011935和Csa015176是miR160的靶基因;在生长素诱导表达的试验中,Csa007296、Csa011935和Csa015176在根、茎和叶中的表达量均高于对照,说明ARF的表达受IAA的正调控;同时,以上3个基因在叶片和雌花花冠中的表达量均是开花第2 d低于开花当天,而在子房中的表达量确是第2天高于开花当天,尤其是Csa011935和Csa015176上调显著,说明ARF在子房发育过程中起到至关重要的作用;ARF在种子萌发过程中的表达分析显示:大部分基因在吸涨后12 h和48 h处于表达最高峰。【结论】ARF受生长素和相应的small RNAs调控。在黄瓜单性结实和种子萌发过程中,ARF可能起到了关键性作用。 | 盛慧 秦智伟 李文滨 周秀艳 武涛 辛明 | 2014 | 中国农业科学2014，47，10： | 14 |
| 17 | α-Asarone suppresses the proliferation and migration of ASMCs through targeting the IncRNA-PVT1/miR-203a/E2F3 signal pathway in RSV-infected rats显示文摘气喘是长期的煽动性的肺的疾病,呼吸 syncytial 病毒(RSV ) 感染是在婴儿和小孩子的更低的呼吸的道病的一个普通原因。-Asarone 介绍许多药理学效果并且被表明了在对待气喘有用。然而,在感染 RSV 的气喘的 -asarone 的功能的机制没被调查。长非编码的 RNA (lncRNAs ) 被报导了在许多生物过程起关键作用。尽管许多 lncRNAs 被描绘了,很少在气喘被报导,特别在导致 RSV 的气喘。当前,一条新奇 post-transcriptional 规定在哪个 lncRNAs 作为竞争内长的 RNA (ceRNAs ) 工作竞争地擦掉 miRNAs 被建议了,从而调整目标基因。在现在的学习,我们建立了一个感染 RSV 的 Sprague-Dawley 老鼠模型并且证明 lncRNA-PVT1 在对待导致 RSV 的气喘,和 lncRNA-PVT1 涉及 -asarone 的机制由作为竞争地擦掉 miR-203a 的 ceRNA 工作调整 E2F3 的表示。 | Xiao Yu Zhe Zhe Binqing Tang Shaobin Li Ling Tang Yingen Wu Xiaorong Chen Hong Fang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 14 |
| 18 | Micro RNAs in colorectal cancer:Role in metastasis and clinical perspectives显示文摘Colorectal cancer(CRC)is the third most common malignancy and the third leading cause of cancer related deaths in the United States.Almost 90%of the patients diagnosed with CRC die due to metastases.Micro RNAs(mi RNAs)are evolutionarily conserved molecules that modulate the expression of their target genes post-transcriptionally,and they may participate in various physiological and pathological processes including CRC metastasis by influencing various factors in the human body.Recently,the role mi RNAs play throughout the CRC metastatic cascade has gain attention.Many studies have been published to link them with CRC metastasis.In this review,we will briefly discuss metastatic steps in the light of mi RNAs,along with their target genes.We will discuss how the aberration in the expression of mi RNAs leads to the formation of CRC by effecting the regulation of their target genes.As mi RNAs are being exploited for diagnosis,prognosis,and monitoring of cancer and other diseases,their high tissue specificity and critical role in oncogenesis make them new biomarkers for the diagnosis and classification of cancer as well as for predicting patients’outcome.Mi RNA signatures have been identified for many human tumors including CRC,and mi RNA-based therapies to treat cancer have been emphasized lately.These will also be discussed in this review. | Shan Muhammad Kavanjit Kaur Rui Huang Qian Zhang Paviter Kaur Hamza Obaid Yazdani Muhammad Umar Bilal Jiang Zheng Liu Zheng Xi-Shan Wang | 2014 | World Journal of Gastroenterology2014，20，45： | 13 |
| 19 | New insights of Helicobacter pylori host-pathogen interactions: The triangle of virulence factors, epigenetic modifications and non-coding RNAs显示文摘Helicobacter pylori(H. pylori) is a model organism for understanding host-pathogen interactions and infection-mediated carcinogenesis. Gastric cancer and H. pylori colonization indicates the strong correlation. The progression and exacerbation of H. pylori infection are influenced by some factors of pathogen and host. Several virulence factors involved in the proper adherence and attenuation of immune defense to contribute the risk of emerging gastric cancer, therefore analysis of them is very important. H. pylori also modulates inflammatory and autophagy process to intensify its pathogenicity. From the host regard, different genetic factors particularly affect the development of gastric cancer. Indeed, epigenetic modifications, Micro RNA and long non-coding RNA received more attention. Generally, various factors related to pathogen and host that modulate gastric cancer development in response to H. pylori need more attention due to develop an efficacious therapeutic intervention. Therefore, this paper will present a brief overview of host-pathogen interaction especially emphases on bacterial virulence factors, interruption of host cellular signaling, the role of epigenetic modifications and non-coding RNAs. | Farzam Vaziri Samira Tarashi Abolfazl Fateh Seyed Davar Siadat | 2018 | World Journal of Clinical Cases2018，6，5： | 12 |
| 20 | 植物长链非编码RNA研究进展显示文摘长链非编码RNA(Long non-coding RNA,lncRNA)长度大于200个核苷酸,大量存在于生物体中并具有多种生物学功能。目前,植物中发现的lncRNA大多由RNA聚合酶Ⅱ转录,并通过目标模仿、转录干扰、组蛋白甲基化和DNA甲基化等多种机制介导基因的表达,在植物开花、雄性不育、营养代谢、生物和非生物胁迫等生物过程中起着调节因子的作用。文章综述了近年来发现的植物lnc RNA数据库、预测方法、表达及可能的生物学功能。 | 黄小庆 李丹丹 吴娟 | 2015 | 遗传2015，37，4： | 11 |
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