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Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

查看全文 作　　者：Dan [1]Zhang;Yan-Bo [2]Ren;Kai [3]Wei;Jue [1]Hong;Yan-Ting [4]Yang;Li-Jie [4]Wu;Ji [4]Zhang;Zheng [1]Shi;Huan-Gan [1]Wu;Xiao-Peng [1,4]Ma 高影响力作者 机构地区：[1]Laboratory of Acupuncture-moxibustion and Immunology,Shanghai Research Institute of Acupuncture and Meridian,Shanghai University of Traditional Chinese Medicine;[2]Department of Integrated Traditional Chinese Medicine and Western Medicine,North Branch of Huashan Hospital,Fudan University;[3]Key Laboratory of Systems Biomedicine (Ministry of Education),Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University;[4]Yueyang Clinical Medicine School,Shanghai University of Traditional Chinese Medicine高影响力机构 出　　处：《World Journal of Gastroenterology》索引2018年第24卷第30期,共14页高影响力期刊 基　　金：Supported by the National Natural Science Foundation of China,No.81674073,81202754,and 81273843;Training Project for Outstanding Discipline Leaders of Shanghai Municipal Commission of Health and Family Planning,No.2017BR047;National Key Basic Research Program of China(973 Program),No.2015CB554501 and 2009CB522900;Budgetary Projectof Shanghai University of Traditional Chinese Medicine,No.18LK050 摘　　要：AIM To observe the effect of herb-partitioned moxibustion(HPM) on expression of colonic cytokines in ulcerative colitis(UC) rats.METHODS A UC rat model was established by protein immunization in combination with topical chemical stimulation.Rats in the HPM group(n = 8) received HPM at bilateral Tianshu(ST25) points.The gross injury and pathological scores of the colon were recorded.The expression profile of colonic cytokines was assayed using the protein microarray technique.Specific differential cytokines were selected and verified by ELISA.The corresponding Uni Prot Accessions of the differentially expressed cytokines were retrieved in the Uni Prot database.The pathways involved were analyzed with the help of the KEGG PATHWAY database.The DAVID database was used for functional cluster and pathway analysis.RESULTS HPM improved colon injuries in UC rats,manifested by accelerated repair of ulcers and alleviation of inflammation,and the gross injury and pathological scores both significantly decreased(P < 0.01).Fold change > 1.3 or < 0.77 was taken as the screening standard.There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group,and expression of 20 differed significantly(P < 0.05).Twelve of the 20 significantly differentially expressed cytokines [β-catenin,interleukin-1 receptor 6(IL-1 R6),IL-1β,B7-1,nerve growth factor receptor,AMP-activated protein kinase-α1,neuropilin-2,orexin A,adipocyte differentiation-related protein,IL-2,Fas and Fas L] were up-regulated in the model group(n = 3,compared with the normal group) but downregulated in the HPM group(n = 3,compared with the model group).Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation.KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways.The pathways that interacted between the cytokines and their receptors accounted for the largest proportion(28 of the downregulated and 5 of the up-regulated cytokines).CONCLUSION HPM promotes the repair of colon injuries in UC rats,which is related to the regulation of several abnormally expressed cytokines. 关 键 词：Cytokine expression profile ULCERATIVE COLITIS Protein microarray RATS Herb-partitioned MOXIBUSTION
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