Identifying changes in the wheat kernel proteome under heat stress using iTRAQ

查看全文 作　　者：Yufeng [1]Zhang;Hongyao [1]Lou;Dandan [1]Guo;Ruiqi [1]Zhang;Meng [1]Su;Zhenghong [1]Hou;Haiying [1]Zhou;Rongqi [1]Liang;Chaojie [1]Xie;Mingshan [1]You;Baoyun [1]Li

机构地区：[1]Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, College of Agronomy and Biotechnology,China Agricultural University

出　　处：《The Crop Journal》索引2018年第6卷第6期,共11页

基　　金：supported by the National Key Research and Development Program of China(2016YFD0100502) 摘　　要：Wheat(Triticum aestivum L.) is one of the three major global food crops. Hightemperature stress can affect its yield and quality. Studies of the effect of hightemperature stress on wheat kernel development are important because they can reveal the stability of wheat quality and lead to the genetic improvement of wheat quality traits. In this study, the isobaric tags for relative and absolute quantitation(iTRAQ)method was adopted to analyze changes in the protein expression profile of wheat cultivars under high temperature stress. The protein content of wheat grain increased under heat stress, while the SDS-sedimentation value and starch content decreased.Grain filling was deficient under high temperature stress, which reduced thousandkernel weight but did not affect wheat kernel length. The 207 differentially expressed proteins identified in Gaocheng 8901 under heat stress were associated with energy metabolism, growth and development, and stress response. Gene Ontology enrichment analysis showed that the annotated proteins that were differentially expressed in Gaocheng 8901 under heat stress were involved mainly in stimulus response, abiotic stress response, stress response, and plasma membrane. A set of 78 differentially expressed proteins were assigned to 83 KEGG signaling/metabolic pathways. KEGG pathway enrichment analysis showed that this set of proteins was significantly enriched in members of 51 pathways, and the proteins participated mainly in protein synthesis in the endoplasmic reticulum, starch and sucrose metabolism, and reaction on ribosomes. Five differentially expressed proteins were involved in protein–protein interaction networks that may greatly influence the yield and quality of wheat grain. In wheat, high-temperature stress leads to a variety of effects on protein expression and may ultimately cause changes in yield and quality. 关键词：WINTER WHEAT Heat stress GO ENRICHMENT KEGG PATHWAY ENRICHMENT Interaction networks

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Identifying changes in the wheat kernel proteome under heat stress using iTRAQ

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