Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

查看全文 作　　者：SHANG [1,2,3]Xiaomei;MA [1,2]Aijun;WANG Xin’[1,2]an;XIA [1,2]Dandan;ZHUANG [1,2]Jiao 机构地区：[1]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding,Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Qingdao 266071,China;[2]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China;[3]College of Marine and Environmental Sciences,Tianjin University of Science and Technology,Tianjin 300457,China 出　　处：《Journal of Oceanology and Limnology》索引2020年第38卷第1期,共12页 基　　金：Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-47-G01);the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS04);the Qingdao Natural Science Foundation(No.12-1-4-12-(1)-jch) 摘　　要：The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.However,our understanding of the TRPV4 in fish remains poor.Therefore,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and CaMBD.The CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and liver.Surprisingly,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts.In addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts.Therefore,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C.semilaevis,which lays a foundation for further study of the functions of the free neuromasts. 关 键 词：transient receptor potential VANILLOID 4 (TRPV4) Cynoglossus SEMILAEVIS gene expression free NEUROMASTS in SITU hybridization