Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals

查看全文 作　　者：Shiyuan [1]Chen;Ziehen [1]Wang;Dong [2]Zhang;Aiming [3,4]Wang;Liangyi [1]Chen;Heping [1]Cheng;Runlong [1]Wu

机构地区：[1]State Key Laboratory of Membrane Biology,Institute of Molecular Medicine,Peking-Tsinghua Center for Life Sciences,PKU-Nanjing Institute of Translational Medicine,Peking University,Beijing 100871,China;[2]Academy of Advanced Interdisciplinary Study,Peking University,Beijing 100871,China;[3]Department of Electronics,Peking University,Beijing 100871,China;[4]State Key Laboratory of Advanced Optical Communication System and Networks,Peking University,Beijing 100871,China

出　　处：《Neuroscience Bulletin》索引2020年第36卷第10期,共9页

基　　金：We thank Dr.Zhe Zhao and Dr.Haitao Wu for helping with the experiments for Fig.2D,and Dr.Weijian Zong for discussion.This work was supported by grants from the National Natural Science Foundation of China(31327901,31570839,61975002,31830036,31821091,and 8182780030);the Major State Basic Research Program of China(2016 YFA0500400 and 2016YFA0500403);and the National Postdoctoral Program for Innovative Talents of China(BX20190011). 摘　　要：An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system levels.The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors.In this brief review,we summarize recent advances in miniature fluorescence microscopy for neuroscience,focusing mostly on two mainstream solutions-miniature single-photon microscopy,and miniature two-photon microscopy.We discuss their technical advantages and limitations as well as unmet challenges for future improvement.Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements,long and complex protocols,and even disease progression and aging. 关键词：Miniature fluorescence microscopy Brain imaging Two-photon microscopy Neuronal information processing

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Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals

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