The chromosome-level Stevia genome provides insights into steviol glycoside biosynthesis

查看全文 作　　者：Xiaoyang [1]Xu;Haiyan [1]Yuan;Xiaqing [2]Yu;Suzhen [1]Huang;Yuming [1]Sun;Ting [1]Zhang;Qingquan [1]Liu;Haiying [1]Tong;Yongxia [1]Zhang;Yinjie [1]Wang;Chunxiao [3]Liu;Lei [4]Wu;Menglan [1]Hou;Yongheng [1]Yang

机构地区：[1]Institute of Botany,Jiangsu Province and Chinese Academy of Sciences/Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014 Jiangsu,China;[2]College of Horticulture,Nanjing Agricultural University,Nanjing 210095 Jiangsu,China;[3]Institute of Pomology,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014 Jiangsu,China;[4]Biomarker Technologies Corporation,Beijing 101300,China

出　　处：《Horticulture Research》索引2021年第8卷第1期,共10页

基　　金：the National Natural Science Foundation of China(31701497 and 31601371);the Natural Science Foundation of Jiangsu Province(BK20160600 and BK20180312),the Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(JSPKLB201801 and JSPKLB201832). 摘　　要：Stevia(Stevia rebaudiana Bertoni)is well known for its very sweet steviol glycosides(SGs)consisting of a common tetracyclic diterpenoid steviol backbone and a variable glycone.Steviol glycosides are 150–300 times sweeter than sucrose and are used as natural zero-calorie sweeteners.However,the most promising compounds are biosynthesized in small amounts.Based on Illumina,PacBio,and Hi-C sequencing,we constructed a chromosome-level assembly of Stevia covering 1416 Mb with a contig N50 value of 616.85 kb and a scaffold N50 value of 106.55 Mb.More than four-fifths of the Stevia genome consisted of repetitive elements.We annotated 44,143 high-confidence protein-coding genes in the high-quality genome.Genome evolution analysis suggested that Stevia and sunflower diverged~29.4 million years ago(Mya),shortly after the whole-genome duplication(WGD)event(WGD-2,~32.1 Mya)that occurred in their common ancestor.Comparative genomic analysis revealed that the expanded genes in Stevia were mainly enriched for biosynthesis of specialized metabolites,especially biosynthesis of terpenoid backbones,and for further oxidation and glycosylation of these compounds.We further identified all candidate genes involved in SG biosynthesis.Collectively,our current findings on the Stevia reference genome will be very helpful for dissecting the evolutionary history of Stevia and for discovering novel genes contributing to SG biosynthesis and other important agronomic traits in future breeding programs. 关键词：BACKBONE insight expanded

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The chromosome-level Stevia genome provides insights into steviol glycoside biosynthesis

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