The histone H3 lysine-27 demethylase Jmjd3 plays a critical role in specific regulation of Th17 cell differentiation

查看全文 作　　者：Zhi [1]Liu;Wei [1]Cao;Longxia [2]Xu;Xi [1]Chen;Yu [1]Zhan;Qian [1]Yang;Sanhong [1]Liu;Pengfei [1]Chen;Yuhang [1]Jiang;Xiaohua [1]Sun;Yu [1]Tao;Yiming [1]Hu;Cuifeng [1]Li;Qi [1]Wang;Ying [1]Wang;Charlie Degui [2]Chen;Yufang [1]Shi;Xiaoren [1,3]Zhang

机构地区：[1]Key Laboratory of Stem Cell Biology,Institute of Health Sciences,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine,Shanghai 200031,China;[2]State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China;[3]Collaborative Innovation Center of Systems Biomedicine,Shanghai Jiao Tong University School of Medicine,Shanghai 200240,China

出　　处：《Journal of Molecular Cell Biology》索引2015年第7卷第6期,共12页

基　　金：supported by grants from the National Basic Research Program(2014CB541904,2011CB946102,and 2014CB943600);the National Natural Science Foundation of China(31370881,90919017,and 30972695);the Knowledge Innovation Project of Chinese Academy of Sciences(KSCX1-YW-22);the CAS-CSIRO Cooperative Research Program(GJHZ1409). 摘　　要：Interleukin(IL)17-producing T helper(Th17)cells play critical roles in the clearance of extracellular bacteria and fungi as well as the pathogenesis of various autoimmune diseases,such as multiple sclerosis,psoriasis,and ulcerative colitis.Although a global transcriptional regulatory network of Th17 cell differentiation has been mapped recently,the participation of epigenetic modifications in the differentiation process has yet to be elucidated.We demonstrated here that histone H3 lysine-27(H3K27)demethylation,predominantly mediated by the H3K27 demethylase Jmjd3,crucially regulated Th17 cell differentiation.Activation of naı¨ve CD41 T cells immediately induced high expression of Jmjd3.Genetic depletion of Jmjd3 in CD41 T cells specifically impaired Th17 cell differentiation both in vitro and in vivo.Ectopic expression of Jmjd3 largely rescued the impaired differentiation of Th17 cells in vitro in Jmjd3-deficientCD41 T cells.Importantly,Jmjd3-deficient mice were resistant to the induction of experimental autoimmune encephalomyelitis(EAE).Furthermore,inhibition of the H3K27 demethylase activity with the specific inhibitor GSK-J4 dramatically suppressed Th17 cell differentiation in vitro.At the molecular level,Jmjd3 directly bound to and reduced the level of H3K27 trimethylation(me3)at the genomic sites ofRorc,which encodes the masterTh17 transcription factorRorgt,and Th17 cytokine genes such as Il17,Il17f,and Il22.Therefore,our studies established acritical role of Jmjd3-mediatedH3K27demethylation inTh17 cell differentiation andsuggest that Jmjd3 can be a novel therapeutic target for suppressing autoimmune responses. 关键词：histone H3K27 demethylation Jmjd3 Th17 cells autoimmune disease

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The histone H3 lysine-27 demethylase Jmjd3 plays a critical role in specific regulation of Th17 cell differentiation

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