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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Comprehensive study on the chemical structure of dioxane lignin from plantation eucalyptus globulus wood 显示文摘 | DMITRY V Evtuguin CARLOS Pascoal Neto ARTUR M S Silva | 2001 | Journal of Agricultural and Food Chemistry2001，49，9： | 1 |
| 3 | Study of the selection mechanism of heavy metal (Pb2 +, Cu2 +, Ni2 +, and Cd2 +) adsorption on clinoptilolite 显示文摘 | Myroslav S Boguslaw B Artur P T | 2006 | Journal of Colloid and Interface Science2006，304，1： | 1 |
| 4 | D -Dimer is an early diagnostic marker of coronary ischemia in patients with chest pain显示文摘 | Antoni Bayes-Genis Jose Mateo Miquel Santaló Artur Oliver Josep Guindo Lina Badimon Antoni Mart??nez-Rubio Jordi Fontcuberta Robert S. Schwartz Antoni Bayés de Luna | 2000 | American Heart Journal2000，，3： | 1 |
| 5 | Anodic treatment of aluminum in nitric acid containing aniline, previous to deposition of polyaniline and its role on corrosion显示文摘 | Huerta-Vilca D Moraes S R Artur J M | 2004 | synth met2004，，140： | 1 |
| 6 | Biogenic amines in meat inoculated with Lactobacillus sake starter strains and an aminepositive lactic acid bacterium显示文摘 | Artur R S Susanna E | 1997 | Z Lebensm Unters Forsch A1997，205，2： | 1 |
| 7 | Experimental demonstration of surface selection rules for SERS on flat metallic surfaces 显示文摘 | LE Ru E C MEYER S ARTUR C | 2011 | Chemical Communications2011，47，13： | 1 |
| 8 | Motion of the foot and ankle during the stance phase in rats显示文摘 | Artur S P Anto'nio | 2002 | Muscle & Nerve2002，26，： | 1 |
| 9 | Utility of a multiprobe fluorescence in situ hybridization assay in the detection of superficial urothelial bladder cancer显示文摘 | Mercedes Marín-Aguilera Lourdes Mengual María José Ribal Moisès Burset Yolanda Arce Elisabet Ars Artur Oliver Humberto Villavicencio Ferran Algaba Antonio Alcaraz | 2007 | Cancer Genetics and Cytogenetics2007，，2： | 1 |
| 10 | Chromones and flavanones from Artemisia campestris subsp maritime 显示文摘 | Joao M J V Artur M S S Jose A S C | 1998 | Phytochemistry1998，49，5： | 1 |
| 11 | Benefits and cost implica-tions from integrating small flexible nuclear reactors with off-shore wind farms in a virtual power plant显示文摘 | S Arturs P Ioulia P | | 0，，01： | 1 |
| 12 | Seasonal and spatial variability of free-living bacterial community eomposition along an estuarine gradient (Ria de Aveiro, Portugal) 显示文摘 | Henriques Isabel S Alves Artur Tac a o Marta | 2006 | Estuarine Coastal and Shelf Science2006，68，12： | 1 |
| 13 | Ammonium sorption from aqueous solutions by the natural zeolite Transcarpathian clinoptilolite studied under dynamic conditions显示文摘 | Myroslav S Mariya L Artur P T | 2005 | Journal of Colloid and Interface Science2005，284，2： | 1 |
| 14 | Molecular mechanisms of primary and secondary mucosal immunity using avian infectious bronchitis virus as a model system显示文摘 | Guo X S Artur R Chen J M | 2008 | Vet Immunol Immunopathol2008，121，34： | 1 |
| 15 | Synthesis and spectral properties of colloidal Nd3 - doped NaYF4 nanocrystals 显示文摘 | Artur B Dominika W Marcin N Wieslaw S | 2011 | Optical Materials2011，33，： | 1 |
| 16 | New approach for management services with a Web browser显示文摘 | Macie J Artur B Maciej S | 1999 | Computer Networks1999，31，21： | 1 |
| 17 | Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal 显示文摘 | Artur T M Kragelund C Eriksen P S | 2012 | Water Res2012，46，12： | 1 |
| 18 | Bacterial cel- lulose membranes as transdermal delivery systems for di- clofenac: In vitro dissolution and permeation studies 显示文摘 | Nuno H C S S Artur F R Isabel F A | 2014 | Carbohydr Polym2014，106，： | 1 |
| 19 | Heat transfer characteristics of a two-phase thermosyphon heat exchanger Original Research Article显示文摘 | Janusz T.Cie(s)li(n)ki Artur Fiu | | 0，，： | 1 |
| 20 | Analysis of mitochondrial Dloop region casts new light on domestic water buffalo (Bubalus bubalis) phylogeny显示文摘 | Gerold K Marcelo V Artur S | 2004 | Mol Phylogenet Evol2004，30，： | 1 |
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