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| 1 | Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs by leucine through an mTORC1-dependent pathway显示文摘Neonatal growth is characterized by a high protein synthesis rate that is largely due to an enhanced sensitivity to the postprandial rise in insulin and amino acids, especially leucine. The mechanism of leucine's action in vivo is not well understood. In this study, we investigated the effect of leucine infusion on protein synthesis in skeletal muscle and liver of neonatal pigs. To evaluate the mode of action of leucine, we used rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) complex-1 (mTORC1). Overnight-fasted 7-day-old piglets were treated with rapamycin for 1 hour and then infused with leucine (400 μmol·kg -1 ·h -1 ) for 1 hour. Leucine infusion increased the rate of protein synthesis, and ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) phosphorylation in gastrocnemius and masseter muscles (P < 0.05), but not in the liver. The leucine-induced stimulation of protein synthesis and S6K1 and 4E-BP1 phosphorylation were completely blocked by rapamycin, suggesting that leucine action is by an mTORC1-dependent mechanism. Neither leucine nor rapamycin had any effect on the activation of the upstream mTORC1 regulators, AMP-activated protein kinase and protein kinase B, in skeletal muscle or liver. The activation of eIF2a and elongation factor 2 was not affected by leucine or rapamycin, indicating that these two pathways are not limiting steps of leucine-induced protein synthesis. These results suggest that leucine stimulates muscle protein synthesis in neonatal pigs by inducing the activation of mTORC1 and its downstream pathway leading to mRNA translation. | Agus Suryawan Hanh V Nguyen Rosemarie D Almonaci Teresa A Davis | 2012 | Journal of Animal Science and Biotechnology2012，3，1： | 9 |
| 2 | Design,economic analysis and environmental consideratiom of minigrid hybrid power systenl with reverse osmosis desalination plant for remote areas显示文摘 | Ahmad Agus Setiawan Yu Zhao Chem V Nayar | 2008 | IEEE Renewable Energy2008，34，： | 1 |
| 3 | Application of the General Stability Index method to assess the quality of butter lettuce during postharvest storage using a multi-quality indices analysis显示文摘 | Ansorena M R Go(n)i M G Agu(e)ro M V | | 0，，03： | 1 |
| 4 | Moisture diffusivity coefficient and convective drying, mdelling of nmrta ( Ugni molinae Turcz) : influence of temperature and vacuum on drying kinetics 显示文摘 | Kong A H Zambra C E Agu~ro J E Antonio V G Roberto Lemus- Mondaca | 2012 | Food and Bioprocess Technology2012，6，4： | 1 |
| 5 | Nanofiltration for drinking water production from deep well water 显示文摘 | Agus K Praptowidodo V S | 2000 | Desalination2000，132，： | 1 |
| 6 | Nanofihration for drinking water production from deep well water 显示文摘 | Agus K Praptowidodo V S | 2000 | Desalination2000，132，13： | 1 |
| 7 | Treatment of advanced non-small cell lung cancer:chemotherapy with or without cisplatin?显示文摘 | Gebbia V Oniga F Agueli R | 2006 | Ann Oncol2006，17，2： | 1 |
| 8 | Effect of germinationtemperatures on proteolysis of the gluten- free grains rice and buckwheat during malting and mashing 显示文摘 | Agu R C Chiba Y Goodfellow V | 2012 | J Agr Food Chem2012，60，10： | 1 |
| 9 | Re:A novel fusion gene,SYT-SSX4,in synovial sarcoma显示文摘 | Agus V Tamborini E Mezzelani A | 2001 | J Natl Cancer Inst2001，93，17： | 1 |
| 10 | Asia-Pacific mussel watch: monitoring contamination of persistent organochlorine compounds in coastal waters of Asian countries显示文摘 | In Monirith Daisuke Ueno Shin Takahashi Haruhiko Nakata Agus Sudaryanto Annamalai Subramanian Subramanian Karuppiah Ahmad Ismail Muswerry Muchtar Jinshu Zheng Bruce J Richardson Maricar Prudente Ngyen Duc Hue Touch Seang Tana Alexander V Tkalin Shinsuke T | 2002 | Marine Pollution Bulletin2002，，3： | 1 |
| 11 | Effect of germination temperatures on proteolysis of the glu- ten-free grains rice and buckwheat during malting and mashing 显示文摘 | Agu R C Chiba Y Goodfellow V | 2012 | J Agr Food Chem2012，60，10： | 1 |
| 12 | Metabolism and absorption enhancement of methionineenkephalin in human nasal epithelium显示文摘 | Agu RU Dang H V Jorissen M | 2004 | Peptides2004，25，4： | 1 |
| 13 | Nasal absorption enhancement strategies for therapeutic peptides:an in vitro study using cultured human nasal epithelium显示文摘 | Agu RU Dang H V Jorissen M | 2002 | Int J Pharm2002，237，12： | 1 |
| 14 | Lack of SYT-SSX fusion transcripts in malignant peripheral nerve sheath tumors on RT-PCR analysis of 34 archival cases 显示文摘 | Tamborini E Agus V Perrone F | 2002 | Lab Invest2002，82，5： | 1 |
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