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| 1 | Conserved arginine residue in the membrane-spanning domain of HIV-1 gp41 is required for efficient membrane fusion显示文摘Despite the high mutation rate of HIV-1,the amino acid sequences of the membrane-spanning domain(MSD)of HIV-1 gp41 are well conserved.Arginine residues are rarely found in single membrane-spanning domains,yet an arginine residue,R696(the numbering is based on that of HXB2),is highly conserved in HIV-1 gp41.To examine the role of R696,it was mutated to K,A,I,L,D,E,N,and Q.Most of these substitutions did not affect the expression,processing or surface distribution of the envelope protein(Env).However,a syncytia formation assay showed that the substitution of R696 with amino acid residues other than K,a naturally observed mutation in the gp41 MSD,decreased fusion activity.Substitution with hydrophobic amino acid residues(A,I,and L)resulted in a modest decrease,while substitution with D or E,potentially negatively-charged residues,almost abolished the syncytia formation.All the fusion-defective mutants showed slower kinetics with the cell-based dual split protein(DSP)assay that scores the degree of membrane fusion based on pore formation between fusing cells.Interestingly,the D and E substitutions did show some fusion activity in the DSP assays,suggesting that proteins containing D or E substitutions retained some fusion pore-forming capability.However,nascent pores failed to develop,due probably to impaired activity in the pore enlargement process.Our data show the importance of this conserved arginine residue for efficient membrane fusion. | Yufei Long Fanxia Meng Naoyuki Kondo Aikichi Iwamoto Zene Matsuda | 2011 | Protein & Cell2011，2，5： | 1 |
| 2 | A Retrospective Study of methicillin-resistant staphylococcus aureus clinical strains in Tokyo university hospital显示文摘 | TAE T KATSUKO O AIKICHI I | 1995 | J Infect Chemother1995，1，： | 1 |
| 3 | Antigen recognition by class I-restricted T lymphocytes显示文摘 | Townsend A Bodmer H Aikichi M | 1989 | Annu Rev Immunol1989，7，： | 1 |
| 4 | CRF01_AE subtype is associated with X4 tropism and fast HIV progression in Chinese patients infected through sexual transmission显示文摘 | Yijia Li Yang Han Jing Xie Lijun Gu Wenjuan Li Huanling Wang Wei Lv Xiaojing Song Yanling Li Jean-Pierre Routy Takaomi Ishida Aikichi Iwamoto Taisheng Li | 2014 | AIDS2014，，4： | 1 |
| 5 | AIDS is not over显示文摘Acquired immunodeficiency syndrome(AIDS)was first reported in 1981[1].Five healthy young men suddenly presented with opportunistic pneumonia caused by Pneumocystis carinii(currently termed P.jirovecii).The original report from Los Angeles was followed by many reports of patients with similar symptoms in large cities in | IWAMOTO Aikichi LIU HuanLiang LI Hong | 2015 | Science China(Life Sciences)2015，58，5： | 1 |
| 6 | 5'flanking and first intron sequences of the human 13-actin gene required for efficient promoter activity 显示文摘 | Robert M F Monette R M Aikichi I | 1989 | Nucl Acids Res1989，17，1： | 1 |
| 7 | Current concepts in SARS treatment显示文摘 | TAKESHI F TETSUYA N AIKICHI I | | 0，，01： | 1 |
| 8 | HIV-1 tropism显示文摘Acquired immunodeficiency syndrome(AIDS)was reported in 1981((CDC),1981).Human immunodeficiency virus type-1(HIV-1),the virus causing AIDS,was isolated in 1983(Barré-Sinoussi et al.,1983).Since AIDS patients were characterized by a profound decrease in CD4-positive T cell count(Gottlieb et al.,1981),it was a logical consequence that CD4 would be the most probable receptor for the virus.Actually,it was confirmed that CD4 is the primary receptor for HIV-1 in 1986(Maddon et al.,1986).However,it was speculated that HIV-1 requires another factor to infect a cell,since CD4 alone could not support HIV-1 infection to nonhuman cells(Weiner et al.,1991). | Aikichi Iwamoto Noriaki Hosoya Ai Kawana-Tachikawa | 2010 | Protein & Cell2010，1，6： | 0 |
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