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| 1 | Molecular characteristics of the omp A gene of serotype B Chlamydia trachomatis in Qinghai Tibetan primary school students显示文摘To study the molecular characteristics of Chlamydia trachomatis, the major outer membrane protein gene(omp A) of C. trachomatis from primary school students with trachoma residing in the Qinghai Tibetan area was sequenced and compared with the same serotype in Gen Bank. In Jianshetang Primary School and Galeng Central Primary School in the Galeng Tibetan Township of Qinghai Haidong Sala Autonomous County, scraped samples were collected from the upper tarsal conjunctiva and lower conjunctival sac of both eyes of 45 students with trachoma, stored at 4°C, and transported to Beijing Tongren Hospital by air within 24 h. The samples were screened for C. trachomatis by real-time PCR. The omp A gene from the C. trachomatis-positive samples was amplified by nested PCR. The serotype was confirmed by National Center for Biotechnology Information(NCBI) BLAST search and homology analysis. The entire omp A gene sequence was compared with the corresponding gene sequences of serotype B strains available in Gen Bank. Of the 45 students aged 6–13 years with trachoma, 26 C. trachomatis-positive students were identified by the initial real-time PCR screening(average age,(9.09±1.63) years; sex ratio, 1.0), accounting for 57.78%(26/45). The cycle threshold values for real-time PCR were 16.79–37.77. Half(13/26) of C. trachomatis-positive students had a bacterial copy number of >105. The compliance rate of the omp A gene sequences with the C. trachomatis serotype B strains in Gen Bank was up to 99%. Two novel genetic mutations were found when the omp A gene was compared with those of the 11 serotype B strains in Gen Bank. The two non-synonymous mutations were located at(i) position 271 in the second constant domain, an adenine(A) to guanine(G) substitution(ACT?GCT), changing the amino acid at position 91 from threonine to alanine(Thr?Ala) in all 26 strains; and(ii) position 887 in the fourth variable domain, a cytosine(C) to thymine(T) substitution(GCA?GTA), changing the amino acid at residue 296 from alanine to valine(Ala?Val) in four of the 26 strains. Six mutations were identified relative to ATCC VR-573. The strains could be divided into two gene clusters according to the mutation at nucleotide position 887: CQZ-1(China Qinghai Tibetan-1) and CQZ-2(China Qinghai Tibetan-2). We thus detected two novel serotype B mutant strains of C. trachomatis among study subjects with trachoma. | Xue Li Shaoya Zhang Qingfeng Liang Mei Wang Ailian Hu Xiuyuan Li Benshan Yang Mingxin Zhang Ningli Wang Xinxin Lu | 2016 | Science China(Life Sciences)2016，59，6： | 6 |
| 2 | Hydrothermal Self-assembly Synthesis of Mn3O4Reduced Graphene Oxide Hydrogel and Its High Electrochemical Performance for Supercapacitors显示文摘在现在的工作 Mn3O4/reduced graphene 氧化物 hydrogel (Mn3O4-rGOH ) 与三维(3D ) 网络被制作由一热水自己组装线路。同样获得的样品的形态学,作文,和微观结构用粉末X光检查衍射( XRD )被描绘,X光检查光电子光谱学( XPS ), thermogravimetry 分析( TG ),原子吸收 spectrometry ( AAS ),回答扫描电子显微镜学( FESEM )和传播电子显微镜( TEM )的排放。而且,电气化学的行为被周期的 voltammogram (CV ) 评估, galvanostatic 费用分泌物和电气化学的阻抗光谱学(EIS ) 。测试结果显示有 6.9% Mn3O4 的 hydrogel 完成了 148 F | Li Li Zhongai Hu Yuying Yang Pengju Liang Ailian Lu Huan Xu Yingying Hu Hongying Wu | 2013 | Chinese Journal of Chemistry2013，31，10： | 6 |
| 3 | Etiological characteristics of chlamydia trachoma conjunctivitis of Primary Boarding School students in the Qinghai Tibetan area显示文摘The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence for further targeted efforts in the prevent of sporadic trachoma efforts. From two primary schools in Qinghai Province, ocular samples from 35 students who were clinically diagnosed as trachoma cases and 60 normal controls were obtained by swabbing their upper eyelids and lower conjunctival sacs. Samples were preserved at 4°C and airlifted to Beijing Tongren Hospital within 24 h. Real-time polymerase chain reaction(RT-PCR) was used to screen for C. trachomatis, and nested PCR was used to amplify a fragment of the omp A gene for serotype confirmation. Bacterial cultivation and sensitivity tests were conducted based on the 2015 version of the Clinical and Laboratory Standards Institute. Adenovirus, herpes simplex virus, cytomegalovirus, and Epstein-Barr virus were screened by RT-PCR. Among the 35 students with trachoma, 8 came from the Jianshetang Primary School and 27 came from the Central Primary School. Two novel C. trachomatis B serotypes(Gen Bank accession numbers KU737520 and KU737521) were detected based on a sequence analysis of the omp A gene. Single C. trachomatis infections accounted for 42.86%(9/21) of the cases, and infections with multiple bacteria, particularly Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, and Streptococcus pneumoniae, accounted for the remaining 57.14%(12/21). Of the 14 C. trachomatis-negative samples, one was positive for adenoviral infection(serotype D) and 13 were positive for bacterial infections(H. influenzae, M. catarrhalis, S. pneumoniae, S. aureus, streptococci other than S. pneumoniae, Staphylococcus epidermidis, Corynebacterium, and Arthrobacterium). In addition to C. trachomatis, the other bacteria and virus that were detected in the boarding students of primary schools in the Qinghai Tibetan area should be emphasized in trachoma prevention and control. | Mei Wang Xinxin Lu Ailian Hu Mingxin Zhang Xue Li Shijing Deng Ningli Wang | 2016 | Science China(Life Sciences)2016，59，6： | 4 |
| 4 | Study of infectious conjunctivitis among children in rural areas of Qinghai province显示文摘Infectious conjunctivitis is no longer a common eye disease in cities and developed areas, but in remote mountainous regions of China where living conditions are relatively under-developed, the occurrence of infectious conjunctivitis remains a public health issue. Here, we evaluate the ratio of infectious conjunctivitis among primary school students in the rural areas of Xunhua, Huangyuan, and Huzhu Counties in the Qinghai province of China, and report the microorganisms involved in causing infection. Of the 765 eligible primary school students, 694 participated to this study(a response rateof 90.7%), 381 boys and 313 girls, with a mean age of 7.52 years(range 7–9 years). A clinical diagnosis of infectious conjunctivitis was returned for 238 of the 694 children examined. The ratio of infectious conjunctivitis in Xunhua County(46.64%) was higher compared to that in Huangyuan(26.67%; P=0.03) and Huzhu Counties(23.61%; P=0.02). The leading cause of conjunctivitis was bacterial, followed by chlamydial and viral. Our results show that there is a high ratio of infectious conjunctivitis among rural primary school students in Qinghai province. | Qingfeng Liang Xinxin Lu Mei Wang Lei Tian Antoine Labbé Ailian Hu | 2016 | Science China(Life Sciences)2016，59，6： | 3 |
| 5 | Enlightenment of blinding trachoma elimination in China显示文摘Trachoma is a blinding eye disease caused by Chlamydia trachomatis infection.Trachoma was pandemic in the 19th century and rampant around the world,and was a widely prevalent infectious eye disease.Still prevalent in some countries and regions,it is an eye disease with the longest epidemic time.Trachoma is a blinding eye disease pandemic globally,having caused many people to lose their visions | Ailian Hu Ningli Wang | 2017 | Science China(Life Sciences)2017，60，12： | 2 |
| 6 | Prevalence of Visual Impairment among Adults in China: The Beijing Eye Study显示文摘 | Liang Xu Tongtong Cui Hua Yang Ailian Hu Ke Ma Yuanyuan Zheng Baochen Sun Jianjun Li Guizhi Fan Jost B. Jonas | 2006 | American Journal of Ophthalmology2006，，3： | 1 |
| 7 | Refractive Error in Urban and Rural Adult Chinese in Beijing显示文摘 | Liang Xu Jianjun Li Tongtong Cui Ailian Hu Guizhi Fan Rongxiu Zhang Hua Yang Baochen Sun Jost B. Jonas | 2005 | Ophthalmology2005，，10： | 1 |
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