| 1 | Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells显示文摘Whereas the critical roles of innate lymphoid cells(ILCs)in adult are increasingly appreciated,their developmental hierarchy in early human fetus remains largely elusive.In this study,we sorted human hematopoietic stem/progenitor cells,lymphoid progenitors,putative ILC progenitor/precursors and mature ILCs in the fetal hematopoietic,lymphoid and non-lymphoid tissues,from 8 to 12 post-conception weeks,for single-cell RNA-sequencing,followed by computational analysis and functional validation at bulk and single-cell levels.We delineated the early phase of ILC lineage commitment from hematopoietic stem/progenitor cells,which mainly occurred in fetal liver and intestine.We further unveiled interleukin-3 receptor as a surface marker for the lymphoid progenitors in fetal liver with T,B,ILC and myeloid potentials,while IL-3RA–lymphoid progenitors were predominantly B-lineage committed.Notably,we determined the heterogeneity and tissue distribution of each ILC subpopulation,revealing the proliferating characteristics shared by the precursors of each ILC subtype.Additionally,a novel unconventional ILC2 subpopulation(CRTH2–CCR9+ILC2)was identified in fetal thymus.Taken together,our study illuminates the precise cellular and molecular features underlying the stepwise formation of human fetal ILC hierarchy with remarkable spatiotemporal heterogeneity. | Chen Liu Yandong Gong Han Zhang Hua Yang Yang Zeng Zhilei Bian Qian Xin Zhijie Bai Man Zhang Jian He Jing Yan Jie Zhou Zongcheng Li Yanli Ni Aiqing Wen Yu Lan Hongbo Hu Bing Liu | 2021 | Cell Research2021，31，10： | 2 |
| 5 | Green Control Measures of Weeds in Wheat Fields in Hetao Irrigation Area along the Yellow River显示文摘This study was conducted to solve the problem of green weed control in wheat fields in Hetao irrigation area among the Yellow River.Based on the observation of the competition between wheat and weeds in areas where weeds occurred seriously in wheat fields in Hetao irrigation area among the Yellow River,we measured the effects of green weed control measures and wheat yield using different wheat varieties,planting densities,different organic fertilizers,different ploughing times,and different mulching methods.The results showed that the emergence of weeds in wheat fields dominated by Chenopodiaceae weeds,grain amaranth and barnyard grass was more than 10 d later than wheat.Weeds were mainly distributed between rows(holes),and the number of plants accounted for 66.6%(drill seeding)and 97.6%(hole seeding),respectively.And the growth of weeds in rows(holes)was weaker,and the fresh weight of individual plants was 39.3%-41.9%lower than that between rows(holes).The ecological weed inhibitory effect was significant in the early stage of wheat growth;and among the green weed control measures,except that different varieties and planting densities caused no significant difference in weed control effect,other measures had obvious weed control effects.Comprehensive comparison showed that the control effects of plant number in black film full-covered hole seeding,conventional film-covered hole seeding,increasing ploughing times,and applying organic fertilizer free of weed seed pollution were 82.3%,71.7%,22.0%,and 8.6%,respectively;the fresh weight control effects of black film full-covered hole seeding,conventional film-covered hole seeding,increasing ploughing times,and applying organic fertilizer free of weed seed pollution were 98.0%,97.1%,23.9%,and 9.6%,respectively;and the fresh weight control effects of black film full-covered hole seeding,conventional film-covered hole seeding and increasing ploughing times increased wheat yield by 69.4%,56.4%and 21.1%,respectively.The technologies in this study can realize the purposes of mechanized green weed control in organic wheat production and low-cost,high-yield,large-scale production. | Lei YANG Jing LIU Ruiqiang WEN Peizhi ZHANG Chang LIU Aiqing WEN Jiancheng ZHANG Chunzhi ZHAO Huijuan ZHANG Hongxu ZHANG Guoqiang LI | 2021 | Agricultural Biotechnology2021，10，6： | 0 |
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