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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | Structure and function of aggrecan显示文摘Aggrecan is the major proteoglycan in the articular cartilage. This molecule is important in the proper functioning of articular cartilage because it provides a hydrated gel structure (via its interaction with hyaluronan and link protein) that endows the cartilage with load-bearing properties. It is also crucial in chondroskeletal morphogenesis during development. Aggrecan is a multimodular molecule expressed by chondrocytes. Its core protein is composed of three globular domains (G1, G2, and G3) and a large extended region (CS) between G2 and G3 for glycosaminoglycan chain attachment. G1 comprises the amino terminus of the core protein. This domain has the same structural motif as link protein. Functionally, the G1 domain interacts with hyaluronan acid and link protein, forming stable ternary complexes in the extracellular matrix. G2 is homologous to the tandem repeats of G1 and of link protein and is involved in product processing. G3 makes up the carboxyl terminus of the core protein. It enhances glycosaminoglycan modification and product secretion. Aggrecan plays an important role in mediating chondrocyte-chondrocyte and chondrocyte-matrix interactions through its ability to bind hyaluronan. | CHRIS KIANI, LIWEN CHEN, YAO JIONG WU, ALBERT J YEE, BURTON B YANG, Sunnybrook and Women’s College Health Sciences Centre and Department of Laboratory Medicine and Patobiology, 2 Department of Surgeny, Faculty of Medicine, University of Toronto, Canada | 2002 | Cell Research2002，12，1： | 27 |
| 3 | The interaction of versican with its binding partners显示文摘Versican belongs to the family of the large aggregating chondroitin sulfate proteoglycans located primarily within theextracellular matrix (ECM). Versican, like other members of its family, has unique N- and C-terminal globular regions,each with multiple motifs. A large glycosaminoglycan-binding region lies between them. This review will begin byoutlining these structures, in the context of ECM proteoglycans. The diverse binding partners afforded to versican byvirtue of its modular design will then be examined. These include ECM components, such as hyaluronan, type Icollagen, tenascin-R, fibulin-1, and -2, fibrillin-1, fibronectin, P- and L-selectins, and chemokines. Versican also binds tothe cell surface proteins CD44, integrin β1, epidermal growth factor receptor, and P-selectin glycoprotein ligand-1.These multiple interactors play important roles in cell behaviour, and the roles of versican in modulating such processesare discussed. | Yao Jiong WU David P. LA PIERRE Jin WU Albert J. YEE Burton B. YANG | 2005 | Cell Research2005，15，7： | 13 |
| 4 | 黔西南卡林型金矿床与区域古油藏的关系:来自流体包裹体气相组成和沥青拉曼光谱特征的证据显示文摘黔西南地区是世界第二大卡林型金矿床集中区,同时也发育有大量古油藏,二者空间关系密切,但是否具有成因联系目前尚不清楚且存在较大争议。本文报道该区典型金矿床与成矿有关的热液矿物中流体包裹体的气相组成和高品位金矿石中沥青的激光拉曼光谱分析结果,以探讨含有机质流体与金成矿作用之间的关系。研究表明,虽然含砷富金黄铁矿、石英、方解石、萤石和雄黄等热液矿物形成于不同的成矿阶段,但其中流体包裹体的有机气相组分种类、含量及相对比例整体较为一致,表明成矿流体中的有机络离子团在成矿作用过程中没有发生明显的分解或重组,进而说明黔西南卡林型金矿成矿作用过程中有机质并未以有机络离子团的形式对金进行有效搬运或其搬运金的能力非常有限。在N2/Ar—CO2/CH4流体来源判别图解中,各蚀变矿物中的流体包裹体气相组成主要落在循环的大气降水区域,经大气水区域向岩浆流体区域延伸,整体上构成一条非常好的线性分布趋势,暗示成矿流体可能起源于深部岩浆但在演化过程中有大量循环大气水的加入。水银洞金矿床的1件石英样品偏离上述趋势线并向有机流体方向偏移,可能反映了流体迁移过程中有机质的加入。该区金矿床的矿石中均不同程度地发育浸染状沥青,不同矿床中沥青的激光拉曼光谱特征基本一致,说明该区金矿床中的沥青具有相同成因。根据沥青激光拉曼光谱特征地质温度计反演计算的成矿早阶段流体温度为317~336℃,明显高于区域古地温温度(160~250℃)以及区域古油藏的流体包裹体均一温度(73~175℃),说明该区卡林型金矿床的成矿热源与区域古地温无关。对区域地质及近年来地球物理与地球化学最新成果的综合分析表明,该区卡林型金矿床可能是叠加在区域古地温场之上的深部岩浆活动远端低温热液成矿作用的产物,与金矿床空间关系密切的有机质可能为来自先存古油藏并沿控矿构造发生逃逸的油气物质裂解产物,金成矿与区域古油藏之间不存在成因联系。 | 靳晓野 李建威 HOFSTRA Albert MARSH Erin 刘建中 杨梧 JIN XiaoYe LI JianWei LIU JianZhong YANG Wu | 2016 | 岩石学报2016，32，11： | 11 |
| 5 | Birth defects in pregestational diabetes:Defect range,glycemic threshold and pathogenesis显示文摘Currently,60 million women of reproductive age(18-44 years old) worldwide,and approximately 3 million American women have diabetes mellitus,and it has been estimated that this number will double by 2030.Pregestational diabetes mellitus(PGD) is a significant public health problem that increases the risk for structural birth defects affecting both maternal and neonatal pregnancy outcome.The most common types of human structural birth defects associated with PGD are congenital heart defects and central nervous system defects.However,diabetes can induce birth defects in any other fetal organ.In general,the rate of birth defects increases linearly with the degree of maternal hyperglycemia,which is the major factor that mediates teratogenicity of PGD.Stringent prenatal care and glycemic control are effective means to reduce birth defects in PGD pregnancies,but cannot reduce the incidence of birth defects to the rate of that is seen in the nondiabetic population.Studies in animal models have revealed that PGD induces oxidative stress,which activates cellular stress signalling leading to dysregulation of gene expression and excess apoptosis in the target organs,including the neural tube and embryonic heart.Activation of the apoptosis signalregulating kinase 1(ASK1)-forkhead transcription factor 3a(Fox O3a)-caspase 8 pathway causes apoptosis in the developing neural tube leading to neural tube defects(NTDs).ASK1 activates the c-Jun-N-Terminal kinase 1/2(JNK1/2),which leads to activation of the unfolded protein response and endoplasmic reticulum(ER) stress.Deletion of the ASK1 gene,the JNK1 gene,or the JNK2 gene,or inhibition of ER stress by 4-Phenylbutyric acid abrogates diabetes-induced apoptosis and reduces the formation of NTDs.Antioxidants,such as thioredoxin,which inhibits the ASK1-Fox O3a-caspase 8 pathway or ER stress inhibitors,may prevent PGD-induced birth defects. | Rinat Gabbay-Benziv E Albert Reece Fang Wang Peixin Yang | 2015 | World Journal of Diabetes2015，6，3： | 10 |
| 6 | Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds显示文摘The Dormancy-associated MADS-box(DAM)gene cluster in peach serves as a key regulatory hub on which the seasonal temperatures act and orchestrate dormancy onset and exit,chilling response and floral bud developmental pace.Yet,how different temperature regimes interact with and regulate the six linked DAM genes remains unclear.Here,we demonstrate that chilling downregulates DAM1 and DAM3–6 in dormant floral buds with distinct patterns and identify DAM4 as the most abundantly expressed one.We reveal multiple epigenetic events,with tri-methyl histone H3 lysine 27(H3K27me3)induced by chilling specifically in DAM1 and DAM5,a 21-nt sRNA in DAM3 and a ncRNA induced in DAM4.Such induction is inversely correlated with downregulation of their cognate DAMs.We also show that the six DAMs were hypermethylated,associating with the production of 24-nt sRNAs.Hence,the chilling-responsive dynamic of the different epigenetic elements and their interactions likely define distinct expression abundance and downregulation pattern of each DAM.We further show that the expression of the five DAMs remains steadily unchanged or continuously downregulated at the ensuing warm temperature after chilling,and this state of regulation correlates with robust increase of sRNA expression,H3K27me3 and CHH methylation,which is particularly pronounced in DAM4.Such robust increase of repressive epigenetic marks may irreversibly reinforce the chillingimposed repression of DAMs to ensure flower-developmental programming free from any residual DAM inhibition.Taken together,we reveal novel information about genetic and epigenetic regulation of the DAM cluster in peach,which will be of fundamental significance in understanding of the regulatory mechanisms underlying chilling requirement and dormancy release,and of practical application for improvement of plasticity of flower time and bud break in fruit trees to adapt changing climates. | Hong Zhu Pao-Yang Chen Silin Zhong Chris Dardick Ann Callahan Yong-Qiang An Steve van Knocker Yingzhen Yang Gan-Yuan Zhong Albert Abbott Zongrang Liu | 2020 | Horticulture Research2020，7，1： | 9 |
| 7 | Nucleic acid-based diagnostics for infectious diseases in public health affairs显示文摘Infectious diseases,mostly caused by bacteria and viruses but also a result of fungal and parasitic infection,have been one of the most important public health concerns throughout human history.The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost.Many kinds of diagnostics have been developed,such as pathogen culture,biochemical tests and serological tests,to help detect and fight against the causative agents of diseases.However,these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results.Nucleic acid-based diagnostics,detecting pathogens through the identification of their genomic sequences,have shown promise to overcome the above limitations and become more widely adopted in clinical tests.Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage.We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics. | Albert Cheung-Hoi Yu Greg Vatcher Xin Yue Yan Dong Mao Hua Li Patrick H.K.Tam Parker Y.L.Tsang April K.Y.Wong Michael H.K.Hui Bin Yang Hao Tang Lok-Ting Lau | 2012 | Frontiers of Medicine2012，6，2： | 8 |
| 8 | One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering显示文摘 | Haoyi Wang Hui Yang Chikdu S. Shivalila Meelad M. Dawlaty Albert W. Cheng Feng Zhang Rudolf Jaenisch | 2013 | Cell2013，，4： | 5 |
| 9 | SciFinder-guided rational design of fluorescent carbon dots for ratiometric monitoring intracellular pH fluctuations under heat shock显示文摘Intracellular pH plays a significant role in various biological processes, including cell proliferation,apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation of pH responsive carbon dots(CDs-pH) for ratiometric intracellular imaging was reported. By using SciFinder database, fluorescent CDs-pH with the required p Kavalue of 6.84 were rationally designed, which is vital important for precise sensing of intracellular pH. As a result, the synthesized CDspH demonstrated robust ability to test pH fluctuations within the physiological range of 5.4-7.4. The CDspH was further utilized for fluorescent ratiometric imaging of pH in living HeLa cells, effectively avoided the influence of autofluorescence from native cellular species. Moreover, real-time monitoring of intracellular pH fluctuation under heat shock was successfully realized. This SciFinder-guided design strategy is simple and flexible, which has a great potential to be used for the development of other types of CDs for various applications. | Haifang Liu Yuanqiang Sun Zhaohui Li Ran Yang Jie Yang Aaron Albert Aryee Xiaoge Zhang Jia Ge Lingbo Qu Yuehe Lin | 2019 | Chinese Chemical Letters2019，30，9： | 3 |
| 10 | Quality and readability of online information resources on insomnia显示文摘 | Yan Ma Albert C. Yang Ying Duan Ming Dong Albert S. Yeung | 2017 | Frontiers of Medicine2017，11，3： | 3 |
| 11 | New scoring system in assessment of Hoffa's fat pad synovitis: A comparative study with established scoring systems显示文摘AIM To investigate the reliability of the established and new scoring methods for Hoffa's fat pad synovitis using magnetic resonance imaging(MRI).METHODS A total of 139 knees of 115 patients who underwent MRI of the knee with and without gadolinium contrast were enrolled in this study. Proton density(PD)-weighted, PD-weighted fat-suppressed(PD-FS), and postcontrast T1-weighted fat-suppressed(T1CE) images were used for evaluation. Using contrast and noncontrast images, our grading method for synovitis was performed to measure synovial thickness and signal intensity changes of the fat pad [Synovial membrane(SM) score], which was compared with the established methods,including MRI Osteoarthritis Knee Score(MOAKS), parapatellar synovitis score,Whole-Organ Magnetic Resonance Imaging Score(WORMS), and suprapatellar effusion diameter. Intraclass correlation coefficients(ICC) for intra and interobserver reproducibility and Spearman correlation coefficients(r) were calculated for the parapatellar synovitis score and each scoring method.RESULTS All of the scores presented substantial to almost perfect intrareliability. Among three readers, effusion diameter had substantial to almost perfect interreliability(ICC = 0.68-0.81) and WORMS had substantial interreliability(ICC = 0.61-0.70).For two out of three readers, there was substantial interreliability for the thickness score in T1CE(ICC = 0.55-0.69), SM scores in T1CE(ICC = 0.56-0.78)and PD-FS(ICC = 0.51-0.79), and parapatellar synovitis score in T1CE(ICC =0.53-0.72). The parapatellar synovitis score was significantly correlated with the thickness score in T1CE(r = 0.70) and the SM score in T1CE(r = 0.81) and PD-FS(r = 0.65).CONCLUSION The newly proposed quantitative thickness score on T1CE and the semiquantitative SM score on T1CE and PD-FS can be useful for Hoffa's fat pad synovitis. | Shigeo Hagiwara Albert Yang Shoichiro Takao Yasuhito Kaneko Taiki Nozaki Hiroshi Yoshioka | 2018 | World Journal of Radiology2018，10，11： | 3 |
| 12 | Late Pliocene–Quaternary evolution of redox conditions in the western Qaidam paleolake (NE Tibetan Plateau) deduced from Mn geochemistry in the drilling core SG-1显示文摘 | Yibo Yang Xiaomin Fang Erwin Appel Albert Galy Minghui Li Weilin Zhang | 2013 | Quaternary Research2013，，3： | 2 |
| 13 | Quaternary paleolake nutrient evolution and climatic change in the western Qaidam Basin deduced from phosphorus geochemistry record of deep drilling core SG-1显示文摘 | Yibo Yang Xiaomin Fang Albert Galy Erwin Appel Minghui Li | 2013 | Quaternary International2013，，： | 2 |
| 14 | ON THE DIFFUSION PHENOMENON OF QUASILINEAR HYPERBOLIC WAVES显示文摘The authors consider the asymptotic behavior of solutions of the quasilinear hyperbolic equation with linear damping u_tt+ u_t - div (a(u)u) = 0, and show that, at least when n ≥ 3, they tend, as t - +∞, to those of the nonlinear parabolic equation v_t - div (a(v)v) = 0, in the sense that the norm ||u(., t) - v(., t) ||L∞ (R^n) of the difference u - v decays faster than that of either u or v. This provides another example of the diffusion phenomenon of nonlinear hyperbolic waves, first observed by Hsiao, L. and Liu Taiping (see [1, 2]). | YANG HAN ALBERT MILANI | 2000 | Chinese Annals of Mathematics,Series B2000，21，1： | 2 |
| 15 | Low power 3.1–10.6 GHz IR-UWB transmitter for Gbps wireless communications显示文摘This paper reviews and discusses the design of a low-power single-full-band (3.1-10.6 GHz) non- carrier impulse-radio ultra wideband (UWB) transmitter featuring 5th-order Gaussian derivative pulse shaping, integrated BPSK modulation, and 2.5 kV whole-chip ESD (electrostatic discharge) protection. The UWB transmitter design has been implemented in a commercial 0.18 μm CMOS technology with a very small die size of 0.25 mm2. The fabricated chips have demonstrated full functionality, extremely low power consumption of 0.14 pJ/p-mV, and an ultra short pulse width of 394 ps. This ESD-protected UWB transmitter has the potential to support wireless streaming to gigabit per second (Gbps). | WANG Xin LIN Lin TANG He ZHAO Hui FANG Qiang LIU Jian FAN SiQiang WANG Albert ZHAO Bin YANG LiWu ZHANG Gary | 2011 | Science China(Information Sciences)2011，54，5： | 2 |
| 16 | MRI-guided Biopsy to Correlate Tissue Specimens with MR Elastography Stiffness Readings in Liver Transplants显示文摘 | Ryan B. Perumpail Josh Levitsky Yi Wang Victoria S. Lee Jennifer Karp Ning Jin Guang-Yu Yang Bradley D. Bolster Saurabh Shah Sven Zuehlsdorff Albert A. Nemcek Andrew C. Larson Frank H. Miller Reed A. Omary | 2012 | Academic Radiology2012，，9： | 2 |
| 17 | Domestic Investors more Informed than Foreign Investors?显示文摘 | Chan K Albert J M Yang Z S | 2008 | Journal of Finance2008，63，1： | 1 |
| 18 | One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering显示文摘 | Hui Yang Haoyi Wang Chikdu S. Shivalila Albert W. Cheng Linyu Shi Rudolf Jaenisch | 2013 | Cell2013，，6： | 1 |
| 19 | Cytoglobin Up-regulated by Hydrogen Peroxide Plays a Protective Role in Oxidative Stress显示文摘 | Dan Li Xiao Qian Chen Wu-Jian Li Yu-Hui Yang Jian-Zhi Wang Albert Cheung Hoi Yu | 2007 | Neurochemical Research2007，，8： | 1 |
| 20 | Association of Intemet search trends with suicide death in Taipei City, Taiwan显示文摘 | Yang Albert C Tsai Shi-jen Huang Norden E | 2011 | Journal of Affective Disorders2011，，132： | 1 |
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