| 2 | The synergy of Helicobacter pylori and lipid metabolic disorders in induction of Th17-related cytokines in human gastric cancer显示文摘Aim:To study the impact of Helicobacter pylori(H.pylori)and lipid metabolic disorder on the expression of Th17-related cytokines in gastric cancer(GC).Methods:GC specimens were randomly collected from 42 patients,of whom 15 had H.pylori infection and 27 were without.Tumor RNA was extracted for reverse transcription quantitative polymerase chain reaction quantification of gene expression.Results:The mRNA levels of interleukin(IL)-6 and leptin,which are known to regulate Th17 differentiation,were upregulated by 20 and 6 folds,respectively,in H.pylori-infected compared to uninfected patients.IL-17A and granulocyte-macrophage colony-stimulating factor,two cytokines produced by Th17 cells,were 5-and 6-fold higher in tumors with H.pylori infection,respectively.Consistently,RORγt,a transcription factor regulating Th17 differentiation,was increased 6-fold in H.pylori-positive vs.negative tumors.Further elevation of RORγt was seen in advanced H.pylori-associated tumors.In addition,H.pylori infection was also associated with enhanced expression of CXCL1(5 folds),chemotactic factor capable of driving bone marrow-derived immature myeloid cells.Interestingly,we observed that H.pylori-associated increase of IL-17A was enhanced in the group with higher plasma triglycerides.Conclusion:The findings demonstrate a cross-talk and synergistic role of H.pylori infection and abnormal lipid metabolism in GC development,at least partly via cooperative induction of Th17 differentiation and activation. | Jie Liu Han Wang Gang Chen Mo Yang Zhi-Xian Wu Russell Erick Ericksen Alice Sze Tsai Wong Weiping Han Jin-Zhang Zeng | 2017 | Journal of Cancer Metastasis and Treatment2017，3，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员