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| 1 | Metabolic and Signaling Aspects Underpinning the Regulation of Plant Carbon Nitrogen Interactions显示文摘除了光和水, CO2 和矿物质元素为植物生长和开发被要求。在这些因素之中,自从综合被需要,氮是批评的氨基酸,它是蛋白质,核苷酸,叶绿素,和众多的其它的造的元素代谢物和细胞的部件。因此,氮被植物在更高的数量要求,在氮的这项投资支持生产糖,器官的酸,和氨基酸的 CO2,水,和无机的氮的使用,生物资源累积的基本积木。这个系统被复杂新陈代谢的机械维持,它根据象光, CO2,和滋养的可获得性那样的环境因素在不同层次被调整。植物经由一个发信号的网络集成这些信号,它包含代谢物以及滋养察觉到的蛋白质。由于它的重要性,许多研究努力被花费了理解碳和氮新陈代谢怎么根据光合作用, photorespiration,和呼吸的率综合、调整。在这篇文章,因此,我们两个都象在 C3 种的照亮的叶子察觉到并且发信号系统一样在碳 / 氮新陈代谢讨论最近的进展并且提供现在被要求以便带我们的理解去高水平的实验的类型的一个观点。 | Adriano Nunes-Nesi Alisdair R. Fernie Mark Stitt | 2010 | Molecular Plant2010，3，6： | 32 |
| 2 | De Novo Domestication:An Alternative Route toward New Crops for the Future显示文摘Current global agricultural production must feed over 7 billion people.However,productivity varies greatly across the globe and is under threat from both increased competitions for land and climate change and associated environmental deterioration.Moreover,the increase in human population size and dietary changes are putting an ever greater burden on agriculture.The majority of this burden is met by the cultivation of a very small number of species,largely in locations that differ from their origin of domestication.Recent technological advances have raised the possibility of de novo domestication of wild plants as a viable solution for designing ideal crops while maintaining food security and a more sustainable lowinput agriculture.Here we discuss how the discovery of multiple key domestication genes alongside the development of technologies for accurate manipulation of several target genes simultaneously renders de novo domestication a route toward crops for the future. | Alisdair R.Fernie Jianbing Yan | 2019 | Molecular Plant2019，12，5： | 32 |
| 3 | The Structure and Function of Major Plant Metabolite Modifications显示文摘Plants produce a myriad of structurally and functionally diverse metabolites that play many different roles in plant growth and development and in plant response to continually changing environmental conditions as well as abiotic and biotic stresses. This metabolic diversity is, to a large extent, due to chemical modification of the basic skeletons of metabolites. Here, we review the major known plant metabolite modifications and summarize the progress that has been achieved and the challenges we are facing in the field. We focus on discussing both technical and functional aspects in studying the influences that various modifications have on biosynthesis, degradation, transport, and storage of metabolites, as well as their bioactivity and toxicity. Finally, we discuss some emerging insights into the evolution of metabolic pathways and metabolite functionality. | Shouchuang Wang Saleh Alseekh Alisdair R.Fernie Jie Luo | 2019 | Molecular Plant2019，12，7： | 13 |
| 4 | Metabolic and Phenotypic Responses of Greenhouse-Grown Maize Hybrids to Experimentally Controlled Drought Stress显示文摘到象干旱一样的不能生活的压力的改编是农业上的重要获得相关庄稼相似玉米。在在干旱是一个很主导的应力因素的气候的地区种的庄稼的提高的干旱公差的开发因此在植物繁殖起一个必要作用。以前的研究证明玉米收益潜力和提高的压力忍耐是联系特点。在这研究,我们为他们在一个温室实验处理干旱应力的能力分析了六个不同玉米混血儿。我们能从从不同机关与新陈代谢的数据在浇得好的条件下面并且在水限制下面测量的 morphophysiological 参数联合数据。这些不同机关拥有了不同代谢物作文,与显示最可观的 metabolome 变化追随者水缺乏的叶片。虽然我们能在干旱应力下面在代谢物层次显示出一般增加,包括在 TCA 周期的氨基酸,糖,糖白酒,和中介变化,这些变化不在以前基于领域试用数据被指定了为干旱容忍或易受影响的玉米混血儿之间是微分的。这里描述的数据与相当不同的生长源于一个温室实验的事实调节与相比这个领域里的可以解释忍耐为什么组织的天赋不能在这研究被证实。然而,我们能加亮象其层次某些生理的特点地相关很好的代谢物一样显示了保存回答到干旱的几代谢物。 | Sandra Witt Luis Galicia Jan Lisec Jill Cairns Axel Tiessen Jose Luis Araus Natalia Palacios-Rojas Alisdair R. Fernie | 2012 | Molecular Plant2012，5，2： | 12 |
| 5 | MicroTom Metabolic Network: Rewiring Tomato Metabolic Regulatory Network throughout the Growth Cycle显示文摘Tomato(Solanum lycopersicum)is a major horticultural crop worldwide and has emerged as a preeminent model for metabolic research.Although many research efforts have focused on the analysis of metabolite differences between varieties and species,the dynamics of metabolic changes during the tomato growth cycle and the regulatory networks that underlie these changes are poorly understood.In this study,we integrated high-resolution spatio-temporal metabolome and transcriptome data to systematically explore the metabolic landscape across 20 major tomato tissues and growth stages.In the resulting MicroTom Metabolic Network,the 540 detected metabolites and their co-expressed genes could be divided into 10 distinct clusters based on their biological functions.Using this dataset,we constructed a global map of the major metabolic changes that occur throughout the tomato growth cycle and dissected the underlying regulatory network.In addition to verifying previously well-established regulatory networks for important metabolites,we identified novel transcription factors that regulate the biosynthesis of important secondary metabolites such as steroidal glycoalkaloids and flavonoids.Our findings provide insights into spatiotemporal changes in tomato metabolism and generate a valuable resource for the study of metabolic regulatory processes in model plants. | Yan Li Yang Chen Lu Zhou Shengjie You Heng Deng Ya Chen Saleh Alseekh Yong Yuan Rao Fu Zixin Zhang Dan Su Alisdair R.Fernie Mondher Bouzayen Tao Ma Mingchun Liu Yang Zhang | 2020 | Molecular Plant2020，13，8： | 10 |
| 6 | The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice显示文摘Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases(PKSs) are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase(OsPKS2) in male reproduction of rice(Oryza sativa). Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT(wild type), ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds.However, unlike pksb/lap5, in which most detected phenolics were substantially decreased, ospks2 accumulated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/lap5, we propose that PKS proteins have functionally diversified during evolution.Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants. | Xiaolei Zhu Jing Yu Jianxin Shi Takayuki Tohge Alisdair R.Fernie Sagit Meir Asaph Aharoni Dawei Xu Dabing Zhang Wanqi Liang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 9 |
| 7 | Genome-wide Dissection of Co-selected UV-B Responsive Pathways in the UV-B Adaptation of Qingke显示文摘Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke. | Xingquan Zeng Hongjun Yuan Xuekui Dong Meng Peng Xinyu Jing Qijun Xu Tang Tang Yulin Wang Sang Zha Meng Gao Congzhi Li Chujin Shu Zexiu Wei Wangmu Qimei Yuzhen Basang Jiabu Dunzhu Zeqing Li Lijun Bai Jian Shi Zhigang Zheng Sibin Yu Alisdair R.Fernie Jie Luo Tashi Nyima | 2020 | Molecular Plant2020，13，1： | 9 |
| 8 | The Metabolic Response of Arabidopsis Roots to Oxidative Stress is Distinct from that of Heterotrophic Cells in Culture and Highlights a Complex Relationship between the Levels of Transcripts, Metabolites, and Flux显示文摘新陈代谢的调整是一重要,但是糟糕理解,到氧化应力的植物的反应的部分。在以前的研究(巴斯斯特等, 2007 ) ,在到由甲蔡醌的氧化应力的正式就职的文化的 Arabidopsis 房间的新陈代谢的反应被描绘。紧急情况幸存策略在 anabolism 的主要新陈代谢大部分在哪个是下面调整的赞成被揭开分解代谢并且抗氧化剂新陈代谢。在整个植物纸巾的反应可能是不同的,我们因此调查了 Arabidopsis 根的反应到甲蔡醌处理,分析有主要以及第二等的新陈代谢的焦点的 transcriptome, metabolome 和关键新陈代谢的流动。用氧化还原作用敏感的 GFP,甲蔡醌引起氧化还原作用不安,这也被显示出,不是就在线粒体,而且在根的 cytosol 和质体。在里面开始的 30 ? 处理的 min,反应类似于房间文化:在 TCA 周期和氨基酸生合成的代谢物有减少, transcriptomic 反应被 DNA 规章的蛋白质的起来规定统治。在 2 和 6 以后 ? 处理的 h,根的反应对房间文化不同。代谢物层次没仍然保持沮丧,但是相反恢复并且在 pyruvate 的情况中,一些氨基酸和脂肪族的 glucosinolates 在控制层次上面显示出稳定的增加。然而,在中央碳新陈代谢的流动的主要变化都没被观察,新陈代谢的抄本主要独立于相应代谢物变化了。一起,结果建议根纸巾能在氧化抑制以后恢复新陈代谢的活动并且为 glycolysis 和氧化戊糖磷酸盐小径加亮潜在地重要的角色。 | Martin Lehmann Markus Schwarzlander Toshihiro Obata Supaart Sirikantaramas Meike Burow Carl Erik Olsen Takayuki Tohge Mark D. Fricker Birger Lindberg Moiler Alisdair R. Fernie Lee J. Sweetlove Miriam Laxa | 2009 | Molecular Plant2009，2，3： | 8 |
| 9 | Combined Transcriptomics and Metabolomics of Arabidopsis thaliana Seedlings Exposed to Exogenous GABA Suggest Its Role in Plants Is Predominantly Metabolic显示文摘 | Albert Batushansky Menny Kirma Nicole Grillich David Toubiana Phuong Anh Pham Ilse Balbo Hillel Fromm Gad Galili Alisdair R. Fernie Aaron Fait | 2014 | Molecular Plant2014，7，6： | 7 |
| 10 | Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade显示文摘Steroidal glycoalkaloids(SGAs) are nitrogen-containing secondary metabolites of the Solanum species,which are known to have large chemical and bioactive diversity in nature.While recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato,the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical formula.Here we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA biosynthesis.In order to elucidate natural diversity of SGA biosynthesis,a total of 169 putative SGAs found in eight tomato accessions(Soianum lycopersicum,S.pimpinellifolium,S.cheesmaniae,S.chmielewskii,S.neorickii,S.peruvianum,S.habrochaites,S.pennellii) and four tissue types were used for correlation analysis.The results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch points.As such this represents a novel strategy for peak annotation for plant secondary metabolites. | Kevin Schwahn Leonardo Perez de Souza Alisdair R.Fernie Takayuki Tohge | 2014 | Journal of Integrative Plant Biology2014，56，9： | 6 |
| 11 | Induction of the AOX1D Isoform of Alternative Oxidase in A. thaliana T-DNA Insertion Lines Lacking Isoform AOX1A Is Insufficient to Optimize Photosynthesis when Treated with Antimycin A显示文摘植物呼吸被二条小径为电子转移描绘到 O2,也就是被连接到 ATP 生产的细胞色素小径(CP ) ,和其他的小径(AP ) ,没有伴随物 ATP,从 ubiquinol 的电子在哪儿,直接经由其他的 oxidase (AOX ) 转到 O2 生产。这条后者小径很好被适合在光过量电子处理,导致优化光合的性能。我们描绘了不表示主要 isoform 的 Arabidopsis thaliana 的 T-DNA-insertion 变异的线, AOX1A。处于标准生长条件,这些植物没由抗霉素 A 通过 CP 显示出电子流动的任何显型,而是限制,它导致 AOX1A 表示在野类型,在 aox1a 大美人异种的叶子导致了 AOX1D 的增加的表情。尽管有在异种的 AOX1D isoform 的增加的存在,抗霉素 A 引起了光合作用的抑制,增加了 ROS,并且当时,最终导致了放大的膜漏和坏死与相比野类型,它被禁止者仅仅在边际地影响。经由 CP 的电子流动什么时候被限制,因此出现 AOX1D 是不能的充分补偿 AOX1A 的损失。抑制的联合学习,联合了到代谢物介绍并且指向了 glycine decarboxylase 建筑群(GDC ) 的 P 蛋白质的表示分析,建议 aox1a 异种试图为 photorespiration 增加他们的能力。然而,给他们的缺乏,在当与抗霉素 A 对待时,任何一个 AOX1D 都不能充分补偿让 AOX1A 的缺乏优化光合作用的表示增加,是吸引人的。我们建议 aox1a 异种能进一步被用来有关对光合的性能和基因表示的 mitochondrial 氧化还原作用的影响证实当前的模型。 | Inga Strodtkotter Kollipara Padmasree Challabathula Dinakar Birgit Speth Pamela S. Niazi Joanna Wojtera Ingo Voss Phuc Thi Do Adriano Nunes-Nesi Alisdair R. Fernie Vera Linke Agepati S. Raghavendra Renate Scheibe | 2009 | Molecular Plant2009，2，2： | 5 |
| 12 | The Past, Present, and Future of Maize Improvement: Domestication, Genomics, and Functional Genomic Routes toward Crop Enhancement显示文摘After being domesticated from teosinte,cultivated maize(Zea mays ssp.mays)spread worldwide and now is one of the most important staple crops.Due to its tremendous phenotypic and genotypic diversity,maize also becomes to be one of the most widely used model plant species for fundamental research,with many important discoveries reported by maize researchers.Here,we provide an overview of the history of maize domestication and key genes controlling major domestication-related traits,review the currently available resources for functional genomics studies in maize,and discuss the functions of most of the maize genes that have been positionally cloned and can be used for crop improvement.Finally,we provide some perspectives on future directions regarding functional genomics research and the breeding of maize and other crops. | Jie Liu Alisdair R.Fernie Jianbing Yan | 2020 | Plant Communications2020，1，1： | 5 |
| 13 | On the role of the tricarboxylic acid cycle in plant productivity显示文摘The tricarboxylic acid(TCA) cycle is one of the canonical energy pathways of living systems, as well as being an example of a pathway in which dynamic enzyme assemblies, or metabolons, are well characterized. The role of the enzymes have been the subject of saturated transgenesis approaches, whereby the expression of the constituent enzymes were reduced or knocked out in order to ascertain their in vivo function.Some of the resultant plants exhibited improved photosynthesis and plant growth, under controlled greenhouse conditions. In addition, overexpression of the endogenous genes, or heterologous forms of a number of the enzymes, has been carried out in tomato fruit and the roots of a range of species, and in some instances improvement in fruit yield and postharvest properties and plant performance, under nutrient limitation, have been reported, respectively. Given a number of variants, in nature, we discuss possible synthetic approaches involving introducing these variants, or at least a subset of them, into plants. We additionally discuss the likely consequences of introducing synthetic metabolons, wherein certain pairs of reactions are artificially permanently assembled into plants, and speculate as to future strategies to further improve plant productivity by manipulation of the core metabolic pathway. | Youjun Zhang Alisdair R.Fernie | 2018 | Journal of Integrative Plant Biology2018，60，12： | 4 |
| 14 | CsbZIP1-CsMYB12 mediates the production of bitter-tasting flavonols in tea plants (Camellia sinensis) through a coordinated activator–repressor network显示文摘Under high light conditions or UV radiation,tea plant leaves produce more flavonols,which contribute to the bitter taste of tea;however,neither the flavonol biosynthesis pathways nor the regulation of their production are well understood.Intriguingly,tea leaf flavonols are enhanced by UV-B but reduced by shading treatment.CsFLS,CsUGT78A14,CsMYB12,and CsbZIP1 were upregulated by UV-B radiation and downregulated by shading.CsMYB12 and CsbZIP1 bound to the promoters of CsFLS and CsUGT78A14,respectively,and activated their expression individually.CsbZIP1 positively regulated CsMYB12 and interacted with CsMYB12,which specifically activated flavonol biosynthesis.Meanwhile,CsPIF3 and two MYB repressor genes,CsMYB4 and CsMYB7,displayed expression patterns opposite to that of CsMYB12.CsMYB4 and CsMYB7 bound to CsFLS and CsUGT78A14 and repressed their CsMYB12-activated expression.While CsbZIP1 and CsMYB12 regulated neither CsMYB4 nor CsMYB7,CsMYB12 interacted with CsbZIP1,CsMYB4,and CsMYB7,but CsbZIP1 did not physically interact with CsMYB4 or CsMYB7.Finally,CsPIF3 bound to and activated CsMYB7 under shading to repress flavonol biosynthesis.These combined results suggest that UV activation and shading repression of flavonol biosynthesis in tea leaves are coordinated through a complex network involving CsbZIP1 and CsPIF3 as positive MYB activators and negative MYB repressors,respectively.The study thus provides insight into the regulatory mechanism underlying the production of bitter-tasting flavonols in tea plants. | Xuecheng Zhao Xiangsheng Zeng Ning Lin Shuwei Yu Alisdair RFernie Jian Zhao | 2021 | Horticulture Research2021，8，1： | 4 |
| 15 | Catabolism of Branched Chain Amino Acids Supports Respiration but Not Volatile Synthesis in Tomato Fruits显示文摘分支链氨基酸 transaminases (BCAT ) 在分支链氨基酸白氨酸,异白氨酸,和缬氨酸的新陈代谢有一个关键角色。这些酶催化合成的最后步和这些氨基酸的降级的起始的步。尽管在植物的分叉的链氨基酸的 biosynthetic 小径广泛地被调查了,很多基因被描绘了,他们在植物的分解代谢完全还没被理解。我们以前在西红柿描绘了分叉的链氨基酸 transaminase 基因家庭,揭示 subcellular 本地化和六基因编码的酶的运动性质。这里,我们在水果开发期间检验了酶的可能的功能。我们进一步描绘了在分叉的链氨基酸 transaminases 的表示不同的转基因的植物 1 和 3,在线 overexpressing 任何一个 BCAT1 或 BCAT3 在在 BCAT1 和 volatiles 的层次缺乏的水果评估呼吸的率。我们测试的份量上经由喂实验的先锋和同位素,在水果 volatiles 的形成的分叉的链氨基酸和他们的相应 keto 酸的重要性。我们的结果不仅第一次在水果呼吸表明分叉的链氨基酸的重要性,而且揭示那 keto 酸,而非氨基酸,是为分叉的链风味 volatiles 的可能的先锋。 | Andrej Kochevenko Wagner L. Araujo Gregory S. Maloney Denise M. Tieman Phuc Thi Do Mark G. Taylor Harry J. Klee Alisdair R. Fernie | 2012 | Molecular Plant2012，5，2： | 4 |
| 16 | A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species显示文摘Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains arduous.Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis thaliana.As an efficient high-throughput transient expression system is currently lacking in the model plant species A.thaliana,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in A.thaliana and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and N.benthamiana.Efficiency of this method was independently verified in three independent research facilities,pointing to the robustness of this technique.Furthermore,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis. | Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie | 2020 | Plant Communications2020，1，5： | 4 |
| 17 | 低糖野生种与高糖栽培种西瓜果实代谢产物组分差异分析显示文摘采用高效液相色谱—串联质谱(UHPLC–MS/MS)技术对西瓜5份具有代表性的材料果实进行代谢产物分析,在此基础上选取栽培西瓜高糖材料‘97103’和野生西瓜低糖材料‘PI296341’,利用气相色谱—质谱联用(GC–MS)方法对主代谢产物进行定量分析。从成熟果实中定量鉴定出58种代谢物,其中22种在‘97103’和‘PI296341’间存在显著差异。韧皮部运输的棉籽糖在野生低糖材料果实中大量滞留,而在栽培高糖材料中极少积累。综合差异代谢物及其代谢途径分析发现,高糖材料‘97103’果实在水苏糖、棉籽糖等光合产物卸载途径及蔗糖积累代谢途径上的代谢产物积累较高;而低糖材料‘PI296341’,则在氨基酸代谢、有机酸代谢途径及6–磷酸葡萄糖等糖代谢途径上产物积累较高。这些发现为野生西瓜进化到栽培种果实品质的代谢产物积累和调控提供了重要依据,也可为提高西瓜品质提供指导。 | 崔霞霞 王亚钦 任毅 Alisdair R Fernie Saleh Alseekh 何洪巨 宫国义 张海英 郭绍贵 张洁 许勇 | 2018 | 园艺学报2018，45，4： | 4 |
| 18 | Is There a Metabolic Requirement for Photorespiratory Enzyme Activities in Heterotrophic Tissues?显示文摘Dear Editor, Photorespiration is an essential metabolic process in leaves that facilitates recovery of carbon lost by the oxygenase reaction of Rubisco and avoids the accumulation of the toxic product,2-phosphoglycolate (2PG) of this reaction (Bauwe et al.,2012).However,there is also evidence to suggest that photorespiration has a more complex role during normal growth than the mere detoxification of 2PG and the recovery of 3-phosphoglycerate (3PGA) (Bauwe et al.,2012).The current view is that photorespiration is an essential mechanism to maintain optimal photosynthesis,normal growth,and development of oxygenic photosynthetic organisms in an oxygen-containing environment (Bauwe et al.,2012). | Adriano Nunes-Nesi Alexandra Florian Andrew Howden Kathrin Jahnke Stefan Timm Hermann Bauwe Lee Sweetlove Alisdair R. Fernie | 2014 | Molecular Plant2014，7，1： | 3 |
| 19 | Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex显示文摘While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across species.In this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of tomato.For this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic genes.The combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid pathway.The combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit ripening.Our results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways. | Takayuki Tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie | 2020 | Molecular Plant2020，13，7： | 3 |
| 20 | Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit显示文摘Improvement of nutritional and organoleptic quality of fruits is a key goal in current strawberry breeding programs.The ratio of sugars to acids is a determinant factor contributing to fruit liking,although different sugars and acids contribute in varying degrees to this complex trait.A segregating F1 population of 95 individuals,previously characterized for several fruit quality characters,was used to map during 2 years quantitative trait loci(QTL)for 50 primary metabolites,L-ascorbic acid(L-AA)and other related traits such as soluble solid content(SSC),titratable acidity(TA),and pH.A total of 133 mQTL were detected above the established thresholds for 44 traits.Only 12.9%of QTL were detected in the 2 years,suggesting a large environmental influence on primary metabolite content.An objective of this study was the identification of key metabolites that were associated to the overall variation in SSC and acidity.As it was observed in previous studies,a number of QTL controlling several metabolites and traits were co-located in homoeology group V(HG V).mQTL controlling a large variance in raffinose,sucrose,succinic acid,and L-AA were detected in approximate the same chromosomal regions of different homoeologous linkage groups belonging to HG V.Candidate genes for selected mQTL are proposed based on their co-localization,on the predicted function,and their differential gene expression among contrasting F1 progeny lines.RNA-seq analysis from progeny lines contrasting in L-AA content detected 826 differentially expressed genes and identified Mannose-6-phosphate isomerase,FaM6PI1,as a candidate gene contributing to natural variation in ascorbic acid in strawberry fruit. | JoséG.Vallarino Delphine M.Pott Eduardo Cruz-Rus Luis Miranda Juan J.Medina-Minguez Victoriano Valpuesta Alisdair R.Fernie JoséF.Sánchez-Sevilla Sonia Osorio Iraida Amaya | 2019 | Horticulture Research2019，6，1： | 3 |
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