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| 1 | Identification of gamyb-4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development显示文摘In higher plants,male reproductive development is a complex biological process that includes cell division and differentiation,cell to cell communication etc.,while the mechanism underlying plant male reproductive development remains less understood.GAMYB encodes a gibberellins acid(GA) inducible transcription factor that is required for the early anther development in rice(Oryza sativa L.).Here,we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon(+2308),causing a frame shift and premature translational termination.Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis.To understand the regulatory role of GAMYB,we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data.These results revealed that the expression of TDR(TAPETUM DEGENERATION RETARDATION),a tapetal cell death regulator,was downregulated in gamyb-4 and udt1(undeveloped tapetum1).While the GAMYB expression was not obviously changed in tdr and udt1-1,and no apparent expression fold change of UDT1 in tdr and gamyb-4,suggesting that TDR may act downstream of GAMYB and UDT1,and GAMYB and UDT1 work in parallel to regulate rice early anther development.This work is helpful in understanding the regulatory network in rice anther development. | Zhenhua Liu Wenjie Bao Wanqi Liang Jingyuan Yin Dabing Zhang | 2010 | Journal of Integrative Plant Biology2010，52，7： | 35 |
| 2 | The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice显示文摘Although AGAMOUS-LIKE6 (AGL6) MADS-box genes are ancient with wide distributions in gymnosperms and angiosperms, their functions remain poorly understood. Here, we show the biological role of the AGL6-1ike gene, OsMADS6, in specifying floral organ and meristem identities in rice (Oryza sativa L.). OsMADS6 was strongly ex- pressed in the floral meristem at early stages. Subsequently, OsMADS6 transcripts were mainly detectable in paleas, lodicules, carpels and the integument of ovule, as well as in the receptacle. Compared to wild type plants, osmads6 mutants displayed altered palea identity, extra glume-like or mosaic organs, abnormal carpel development and loss of floral meristem determinacy. Strikingly, mutation of a SEPALLATA (SEP)-like gene, OsMADS1 (LHS1), enhanced the defect of osmads6 flowers, and no inner floral organs or glume-like structures were observed in whorls 2 and 3 of osmadsl-z osmads6-1 flowers. Furthermore, the osmadsl-z osmads6-1 double mutants developed severely indetermi- nate floral meristems. Our finding, therefore, suggests that the ancient OsMADS6 gene is able to specify 'floral state' by determining floral organ and meristem identities in monocot crop rice together with OsMADS1. | Haifeng Li Wanqi Liang Ruidong Jia Changsong Yin Jie Zong Hongzhi Kong Dabing Zhang | 2010 | Cell Research2010，20，3： | 33 |
| 3 | Development of japonica Photo-Sensitive Genic Male Sterile Rice Lines by Editing Carbon Starved Anther Using CRISPR/Cas9显示文摘Rice is one of the most important crops as it supports over25%of total caloric intake for humans(Kusano et al.,2015).The world population reached 7.3 billion in 2015 and is projected to reach 8.5 billion in 2030(Word Population Prospects:2015 Revision).To meet the food demands for the everincreasing population with limited arable land,scarce | Quanlin Li Dabing Zhang Mingjiao Chen Wanqi Liang Jiaojun Wei Yiping Qi Zheng Yuan | 2016 | Journal of Genetics and Genomics2016，43，6： | 26 |
| 4 | Rice CYP703A3, a cytochrome P450 hydroxylase, is essential for development of anther cuticle and pollen exine显示文摘Anther cuticle and pollen exine act as protective envelopes for the male gametophyte or pollen grain, but the mechanism underlying the synthesis of these lipidic polymers remains unclear. Previously, a tapetum‐expressed CYP703A3, a putative cytochrome P450 fatty acid hydroxylase, was shown to be essential for male fertility in rice(Oryza sativa L.). However,the biochemical and biological roles of CYP703A3 has not been characterized. Here, we observed that cyp703a3‐2 caused by one base insertion in CYP703A3 displays defective pollen exine and anther epicuticular layer, which differs from Arabidopsis cyp703a2 in which only defective pollen exine occurs. Consistently, chemical composition assay showed that levels of cutin monomers and wax components were dramatically reduced in cyp703a3‐2 anthers. Unlike the wide range of substrates of Arabidopsis CYP703A2, CYP703A3 functions as an in‐chain hydroxylase only for a specific substrate, lauric acid, preferably generating 7‐hydroxylated lauric acid. Moreover, chromatin immunoprecipitation and expression analyses revealed that the expression of CYP703A3 is directly regulated by Tapetum Degeneration Retardation, a known regulator of tapetum PCD and pollen exine formation. Collectively, our results suggest that CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants. | Xijia Yang Di Wu Jianxin Shi Yi He Franck Pinot Bernard Grausem Changsong Yin Lu Zhu Mingjiao Chen Zhijing Luo Wanqi Liang Dabing Zhang | 2014 | Journal of Integrative Plant Biology2014，56，10： | 21 |
| 5 | OsMADS16 Genetically Interacts with OsMADS3 and OsMADS58 in Specifying Floral Patterning ~in Rice显示文摘瑞斯(Oryza sativa ) 有贡献谷物产量的唯一的花的模式。然而,在米饭位于花的机关身份的说明下面的分子的机制,特别地在花的 homeotic 基因之中的相互作用,遗体糟糕理解。这里,我们证明花的 homeotic 基因 OsMADS16 (也叫的 SUPERWOMAN1, SPW1, B 班基因) 和在指定花的机关 patterning 的米饭 C 班基因 OsMADS3 和 OsMADS58 的行为。OsMADS16 和二 C 班基因在第三个锭盘创始人房间有一个重叠表示模式。与单个异种相比, spw1-1 osmads3-4 和 spw1-1 osmads58 加倍异种在花以内展出像颖的机关的另外的锭盘,特别地,六像颖的结构的额外的锭盘在野类型的雄蕊的位置形成了。这些宫外的像颖的结构被显示用标记基因通过细胞的观察并且在 situ 杂交分析有 palea 身份。我们的结果建议 B 班和 C 班基因在在花的分裂组织以内压制不确定的生长起一个关键作用,特别地 whorl-3 primordia。我们也假设那,与以前的假设相对照,在米饭的专业化小穗状花小穗机关, palea,在 eudicots 的萼片的对应物,和词根对苞相应。 | Dapeng Yun Wanqi Liang Ludovico Dreni Changsong Yin Zhigang Zhou Martin M. Kater Dabing Zhang | 2013 | Molecular Plant2013，6，3： | 11 |
| 6 | Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development显示文摘在繁殖开发期间,米饭植物开发决定最后的谷物收益的唯一的花器官。OsMADS1,象 SEPALLATA 一样疯盒子的基因之一,被解开了玩关键角色 inrice 花的机关身份说明和花的分裂组织确定性。然而,在调整花开发与另外的花的 homeotic 基因位于 OsMADS1 的相互作用下面的分子的机制 remainslargely 逃犯。在这个工作,我们在他们的蛋白质之中与物理相互作用一起与 B- , C- ,和 D 班基因学习了 OsMADS1 的基因相互作用。我们证明在 OsMADS1 和 OsMADS3 之间的物理、基因的相互作用为花的分裂组织活动维护和机关身份说明是必要的;当 OsMADS1 身体上并且遗传上在调整花的分裂组织确定性和 suppressingspikelet 分裂组织回复与 OsMADS58 交往时。我们提供了重要基因证据在花开发期间支持二米饭 C 班基因(OsMADS3 和 OsMADS58 ) 的 neofunctionalization。基因表示介绍和量的 RT-PCR 分析进一步表明 OsMADS1 影响涉及发信号的花的身份和荷尔蒙的许多基因的表达式,并且染色质 immunoprecipitation (芯片)-PCR 试金进一步证明 OsMADS17 是 OsMADS1 的直接目标基因。一起拿,这些结果表明 OsMADS1 在指定米饭有多样化的规章的功能花的机关和分裂组织身份,可能通过它和不同花的 homeotic 管理者的基因、物理的相互作用。 | Yun Hu Wanqi Liang Changsong Yin Xuelian Yang Baozhe Ping Anxue Li Ru Jia Mingjiao Chen Zhijing Luo Qiang Cai Xiangxiang Zhao Dabing Zhang Zheng Yuan | 2015 | Molecular Plant2015，8，9： | 10 |
| 7 | The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice显示文摘Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases(PKSs) are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase(OsPKS2) in male reproduction of rice(Oryza sativa). Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT(wild type), ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds.However, unlike pksb/lap5, in which most detected phenolics were substantially decreased, ospks2 accumulated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/lap5, we propose that PKS proteins have functionally diversified during evolution.Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants. | Xiaolei Zhu Jing Yu Jianxin Shi Takayuki Tohge Alisdair R.Fernie Sagit Meir Asaph Aharoni Dawei Xu Dabing Zhang Wanqi Liang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 9 |
| 8 | The Post-meiotic Deficicent Anther1 (PDA1) gene is required for post-meiotic anther development in rice显示文摘To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1 (pda1) from a γ-ray-treated rice mutant library.Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene.The pda1 mutant anther seemed smaller with white appearance.Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis.However,the pda1 mutant displayed obvious defects in postmeiotic tapetal development,abnormal degeneration occurred in the tapetal cells at stage 9 of anther development.Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant,causing no obvious pollen exine formation.RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB,OsC4 and Wax-deficient anther1 (WDA1) was greatly reduced in the pda1 mutant anther.Using map-based cloning approach,the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F2 population.The physical distance between HLF610 and HLF627 was about 194 kb.This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice. | Lifang Hu Hexin Tan Wanqi Liang Dabing Zhang | 2010 | Journal of Genetics and Genomics2010，37，1： | 8 |
| 9 | MYB56 Encoding a R2R3 MYB Transcription Factor Regulates Seed Size in Arabidopsis thaliana显示文摘Plant seed size is tightly regulated by the development of seed coat,embryo,and endosperm;however,currently,its underlying mechanism remains unclear.In this study,we revealed a regulatory role of an R2R3 MYB transcription factor MYB56 in controlling seed size specifcally in Arabidopsis thaliana L.Loss-of-function or knock-down of MYB56 yielded smaller seeds as compared with the wild type.Conversely,overexpression of MYB56 produced larger seeds.Further observation using semi-thin sections showed that myb56 developed smaller contracted endothelial cells and reduced cell number in the outer integument layer of the seed coat during the seed development;by contrast,MYB56 overexpressing lines had expanded endothelial cells and increased cell number in the outer integument layer of the seed coat,suggesting the essential role of MYB56 in regulating seed development.In addition,reciprocal cross-analysis showed that MYB56 affected the seed development maternally.MYB56 was shown to be dominantly expressed in developing seeds,consistently with its function in seed development.Moreover,quantitative reverse transcription polymerase chain reaction analysis revealed that MYB56 regulates the expression of genes involved in cell wall metabolism such as cell division and expansion.Altogether,our results demonstrated that MYB56 represents an unknown pathway for positively controlling the seed size. | Yanjie Zhang Wanqi Liang Jianxin Shi Jie Xu Dabing Zhang | 2013 | Journal of Integrative Plant Biology2013，55，11： | 8 |
| 10 | Regulatory network and genetic interactions established by OsMADS34 in rice inflorescence and spikelet morphogenesis显示文摘Grasses display highly diversified inflorescence architectures that differ in the arrangement of spikelets and flowers and determine cereal yields. However, the molecular basis underlying grass inflorescence morphogenesis remains largely unknown. Here we investigate the role of a functionally diversified SEPALLATA MADS-box transcription factor, OsMADS34, in regulating rice(Oryza sativa L.)inflorescence and spikelet development. Microarray analysis showed that, at the very early stages of inflorescence formation, dysfunction of OsMADS34 caused altered expression of 379 genes that are associated with protein modification and degradation, transcriptional regulation,signaling and metabolism activity. Genetic analysis revealed that OsMADS34 controls different aspects of inflorescence structure, branching and meristem activity synergistically with LAX PANICLE1(LAX1) and FLORAL ORGAN NUMBER4(FON4), as evidenced by the enhanced phenotypes of osmads34 lax1 and osmads34 fon4 compared with the single mutants. Additionally, double mutant between osmads34 and the sterile lemma defective mutant elongated empty glume(ele) displayed an enhanced phenotype, that is,longer and wider sterile lemmas that were converted into lemma/palea-like organs, suggesting that ELE and OsMADS34 synergistically control the sterile lemma development. OsMADS34 may act together with OsMADS15 in controlling sterile lemma development. Collectively, these findings provide insights into the regulatory function of OsMADS34 in rice inflorescence and spikelet development. | Qingcai Meng Xiaofeng Li Wanwan Zhu Li Yang Wanqi Liang Ludovico Dreni Dabing Zhang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 5 |
| 11 | The DNA Topoisomerase VI-B Subunit OsMTOPVIB Is Essential for Meiotic Recombination Initiation in Rice显示文摘 | Ming Fu ChongWang Feiyang Xue James Higgins Mingjiao Chen Dabing Zhang Wanqi Liang | 2016 | Molecular Plant2016，9，11： | 4 |
| 12 | Immunogenicity of recombinant F4 (K88) fimbrial adhesin FaeG expressed in tobacco chloroplast显示文摘到通常对腹泻在植物生产新奇疫苗的可能性在新生、最新断奶的小猪发现了的测试, faeG 基因,编码主要 F4ac fimbrial 子单元蛋白质,被介绍进烟草叶绿体染色体。在在 spectinomycin 下面的二轮选择以后,我们将近获得了转基因的植物 homoplasmic。RNA 胶化污点分析显示了那 faeG 和抗菌素选择基因 aminoglycoside 3 adenylyltransferase (aadA ) 高度作为 dicistron 被抄录,当在 transplastomic 烟草的 recombinant FaeG 的翻译水平是大约 0.15% 全部的可溶的蛋白质时。在烟草叶绿体生产的 recombinant FaeG 的 immunogenicity 被 FaeG 特定的抗体在与导出叶绿体的 recombinant FaeG 刺激的老鼠 sera 能抵销的转基因的植物,以及结果的全部的可溶的蛋白质使免疫的老鼠被得到的观察证实在 vivo 的 F4ac enterotoxigenic Escherichia coli (ETEC ) 。这研究为用叶绿体表达式系统生产 ETEC 疫苗提供一种新选择。 | Huifeng Shen Bingjun Qian Weiwei Chen Zhenhua Liu Litao Yang Dabing Zhang Wanqi Liang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 3 |
| 13 | Fine Mapping and Analysis of DWARF TILLER1 in Controlling Rice Architecture显示文摘Rice is one of the most consumed staple food plants around the world,and its plant architecture is very important to improve the grain yield(Zhang et al.,2008).Plant height,leaf angle,tiller number and angle,and uniformity of panicle layer all can have strong effects on grain yield(Wang and Li,2008).During the long history of domestication,rice has been selected to develop uniform tiller height architecture that ensures panicle layer uniformity and ease of harvesting(Ma et al.,2009),and is largely determined by the | Wenfei Wang Huangwei Chu Dabing Zhang Wanqi Liang | 2013 | Journal of Genetics and Genomics2013，40，9： | 3 |
| 14 | Rice Glucose 6-Phosphate/Phosphate Translocator 1 is required for tapetum function and pollen development显示文摘In plants, non-green plastids in heterotrophic tissues are sites for starch and fatty acids biosynthesis,which are essential for plant development and reproduction. Distinct from chloroplasts, the metabolites for these processes in non-green plastids have to be imported through specific transporters. Glucose 6-Phosphate/Phosphate Translocator 1 is required for the uptake of cytosolic Glucose 6-Phosphate into non-green plastids. In Arabidopsis, GPT1 has been demonstrated to play essential roles in male, female gametophyte and embryo development. However, the roles of GPTs in other species are yet largely unknown. Here, we reported that rice OsGPT1 is indispensable for normal tapetal degeneration and pollen exine formation during anther and pollen development. OsGPT1 is localized in the plastid and distributed in the anther wall layers and late-stage pollen grains. Different from the gametic defects caused by mutation in At GPT1, disruption of OsGPT1 does not affect male and female gamete transmission as well as embryo development. On the contrary, osgpt1 mutant exhibits delayed tapetum degeneration,decreased Ubisch bodies formation and thinner pollen exine, leading to pollen abortion at the mature stage. Furthermore, the expression of several genes involved in tapetal programmed cell death(PCD)and sporopollenin formation is decreased in osgpt1. Our study suggests that OsGPT1 coordinates the development of anther sporophytic tissues and the male gametophyte by integrating carbohydrate and fatty acid metabolism in the plastid. | Weidan Zhang Huanjun Li Feiyang Xue Wanqi Liang | 2021 | The Crop Journal2021，9，6： | 2 |
| 15 | Genome-wide analysis of the barley non-specific lipid transfer protein gene family显示文摘Non-specific lipid transfer proteins(nsLTPs) are small, basic proteins that are characterized by an eight-cysteine motif. The biological functions of these proteins have been reported to involve plant reproduction and biotic or abiotic stress response. With the completion of the barley genome sequence, a genome-wide analysis of nsLTPs in barley(Hordeum vulgare L.)(HvLTPs) will be helpful for understanding the function of nsLTPs in plants. We performed a genome-wide analysis of the nsLTP gene family in barley and identified 70 nsLTP genes,which can be divided into five types(1, 2, C, D, and G). Each type of nsLTPs shares similar exon and intron gene structures. Expression analysis showed that barley nsLTPs have diverse expression patterns, revealing their various roles. Our results shed light on the phylogenetic relationships and potential functions of barley nsLTPs and will be useful for future studies of barley development and molecular breeding. | Mengyue Zhang Yujin Kim Jie Zong Hong Lin Anne Dievart Huanjun Li Dabing Zhang Wanqi Liang | 2019 | The Crop Journal2019，7，1： | 2 |
| 16 | Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice显示文摘理解扩大多拷贝 microRNA (miRNA ) 在植物的家庭,我们局部性从到他们的染色体的 Arabidopsis 和米饭的报导 miRNA 基因,分别地并且从 Arabidopsis 观察了 117 miRNA 基因中的那 37% 个,从米饭的 173 miRNA 基因中的 35% 个是在染色体的部分复制。为了描绘表示多样化是否多发生在植物之中,拷贝 miRNA 家庭成员,我们设计了在 Arabidopsis 和米饭从 10 个家庭指向 48 位预言的 miRNA 先锋的 PCR 教材。从 RT-PCR 数据的结果建议在一样的 miRNA 家庭以内的成员的抄录先锋在不同表示层次是在场的。另外,尽管 miR160 和 miR162 序列在 Arabidopsis 和米饭被保存,我们发现这些基因的表示模式在二种之间不同。这些数据建议那表达式多样化发生了在多拷贝 miRNA 家庭,在植物增加我们 miRNAs 的表达式规定的理解。 | Danhua Jiang Changsong Yin Aiping Yu Xiaofan Zhou Wanqi Liang Zheng Yuan Yun Xu Qingbo Yu Tieqiao Wen Dabing Zhang | 2006 | Cell Research2006，16，5： | 2 |
| 17 | The SEPALLATA-Like Gene OsMADS34 Is Required for Rice Inflorescence and Spikelet Development 显示文摘 | XINGCHUN GAO WANQI LIANG CHANGSONG YIN | 2010 | Plant Physiology2010，153，： | 1 |
| 18 | Production of FaeG, the Major Subunit of K88 Fimbriae, in Transgenic Tobacco Plants and Its Immunogenicity in Mice 显示文摘 | Yahong Huang Wanqi Liang Aihu Pan | 2003 | Infect Immun2003，71，9： | 1 |
| 19 | Transcriptome profiling reveals phase-specific gene expression in the developing barley inflorescence显示文摘The shape of an inflorescence varies among cereals,ranging from a highly branched panicle in rice to a much more compact spike in barley(Hordeum vulgare L.)and wheat(Triticum aestivum L.).However,little is known about the molecular basis of cereal inflorescence architecture.We profiled transcriptomes at three developmental stages of the barley main shoot apex—spikelet initiation,floral organ differentiation,and floral organ growth—and compared them with those from vegetative seedling tissue.Transcript analyses identified 3688 genes differentially transcribed between the three meristem stages,with a further 1394 genes preferentially expressed in reproductive compared with vegetative tissue.Coexpression assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters,revealing distinct patterns for genes such as transcription factors,histone modification,and cell-cycle progression specific for each stage of inflorescence development.We also compared expression patterns of VRS(SIX-ROWED SPIKE)genes and auxin-,gibberellic acid-and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet fertility.Our findings reveal barley inflorescence phase-specific gene expression,identify new candidate genes that regulate barley meristem activities and flower development,and provide a new genetic resource for further dissection of the molecular mechanisms of spike development. | Huiran Liu Gang Li Xiujuan Yang Hendrik N.J.Kuijer Wanqi Liang Dabing Zhang | 2020 | The Crop Journal2020，8，1： | 1 |
| 20 | Oral immunization of mice with plant - derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Eseherichia coli-specific immune responses显示文摘 | Wanqi Liang Yahong Huang Xinghong Yang | 2006 | Medical Microbiology2006，46，3： | 1 |
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