|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis显示文摘Salicylic acid(SA)is an important phytohormone mediating both local and systemic defense responses in plants.Despite over half a century of research,how plants biosynthesize SA remains unresolved.In Arabidop-sis,a major part of SA is derived from isochorismate,a key intermediate produced by the isochorismate syn-thase,which is reminiscent of SA biosynthesis in bacteria.Whereas bacteria employ an isochorismate pyru-vate lyase(IPL)that catalyzes the turnover of isochorismate to pyruvate and SA,plants do not contain an IPL ortholog and generate SA from isochorismate through an unknown mechanism.Combining genetic and biochemical approaches,we delineated the SA biosynthetic pathway downstream of isochorismate in Ara-bidopsis.We found that PBS3,a GH3 acyl adenylase-family enzyme important for SA accumulation,catalyzes ATP-and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate,isochorismoyl-glutamate A.Moreover,we discovered that EPS1,a BAHD acyltransferase-family protein with a previously implicated role in SA accumulation upon pathogen attack,harbors a noncanonical active site and an unprecedented isochorismoyl-glutamate A pyruvoyl-glutamate lyase activity that produces SA from the isochorismoyl-glutamate A substrate.Together,PBS3 and EPS1 form a two-step metabolic pathway to produce SA from isochorismate in Arabidopsis,which is distinct from how SA is biosynthesized in bacteria.This study closes a major knowledge gap in plant SA meta-bolism and would help develop new strategies for engineering disease resistance in crop plants. | Michael P.Torrens-Spence Anastassia Bobokalonova Valentina Carballo Christopher MGlinkerman Toma(s)Pluskal Amber Shen Jing-Ke Weng | 2019 | Molecular Plant2019，12，12： | 10 |
| 2 | Single vs dual(en bloc) kidney transplants from donors ≤ 5 years of age: A single center experience显示文摘AIM: To compare outcomes between single and dual en bloc(EB) kidney transplants(KT) from small pediatric donors. METHODS: Monocentric nonprospective review of KTs from pediatric donors ≤ 5 years of age. Dual EB KT was defined as keeping both donor kidneys attached tothe inferior vena cava and aorta, which were then used as venous and arterial conduits for the subsequent transplant into a single recipient. Donor age was less useful than either donor weight or kidney size in decision-making for kidney utilization as kidneys from donors < 8 kg or kidneys < 6 cm in length were not transplanted. Post-transplant management strategies were standardized in all patients.RESULTS: From 2002-2015, 59 KTs were performed including 34 dual EB and 25 single KTs. Mean age of donors(17 mo vs 38 mo, P < 0.001), mean weight(11.0 kg vs 17.4 kg, P = 0.046) and male donors(50% vs 84%, P = 0.01) were lower in the dual EB compared to the single KT group, respectively. Mean cold ischemia time(21 h), kidney donor profile index(KDPI; 73% vs 62%) and levels of serum creatinine(SCr, 0.37 mg/d L vs 0.49 mg/d L, all P = NS) were comparable in the dual EB and single KT groups, respectively. Actuarial graft and patient survival rates at 5-years follow-up were comparable. There was one case of thrombosis resulting in graft loss in each group. Delayed graft function incidence(12% dual EB vs 20% single KT, P = NS) was slightly lower in dual EB KT recipients. Initial duration of hospital stay(mean 5.4 d vs 5.6 d) and the one-year incidences of acute rejection(6% vs 16%), operative complications(3% vs 4%), and major infection were comparable in the dual EB and single KT groups, respectively(all P = NS). Mean 12 mo SCr and abbreviated MDRD levels were 1.17 mg/d L vs 1.35 mg/d L and 72.5 m L/min per 1.73 m^2 vs 60.5 m L/min per 1.73 m^2(both P = NS) in the dual EB and single KT groups, respectively. CONCLUSION: By transplanting kidneys from young pediatric donors into adult recipients, one can effectively expand the limited donor pool and achieve excellent medium-term outcomes. | Yousef Al-Shraideh Umar Farooq Hany El-Hennawy Alan C Farney Amudha Palanisamy Jeffrey Rogers Giuseppe Orlando Muhammad Khan Amber Reeves-Daniel William Doares Scott Kaczmorski Michael D Gautreaux Samy S Iskandar Gloria Hairston Elizabeth Brim Margaret Mangus Robert J Stratta | 2016 | World Journal of Transplantation2016，6，1： | 3 |
| 3 | Antibiotic-loaded phosphatidylcholine inhibits staphylococcal bone infection显示文摘AIM: To test antibiotic-loaded coating for efficacy in reducing bacterial biofilm and development of osteomyelitis in an orthopaedic model of implant infection. METHODS: Phosphatidylcholine coatings loaded with 25% vancomycin were applied to washed and sterilized titanium wires 20 mm in length. A 10 mm segment was removed from rabbit radius(total = 9; 5 coated, 4 uncoated), and the segment was injected with 1 × 106 colony forming units(CFUs) of Staphylococcus aureus(UAMS-1 strain). Titanium wires were inserted throughthe intramedullary canal of the removed segment and into the proximal radial segment and the segment was placed back into the defect. After 7 d, limbs were removed, X-rayed, swabbed for tissue contamination. Wires were removed and processed to determine attached CFUs. Tissue was swabbed and streaked on agar plates to determine bacteriological score.RESULTS: Antibiotic-loaded coatings resulted in significantly reduced biofilm formation(4.7 fold reduction in CFUs; P < 0.001) on titanium wires and reduced bacteriological score in surrounding tissue(4.0 ± 0 for uncoated, 1.25 ± 0.5 for coated; P = 0.01). Swelling and pus formation was evident in uncoated controls at the 7 d time point both visually and radiographically, but not in antibiotic-loaded coatings.CONCLUSION: Active antibiotic was released from coated implants and significantly reduced signs of osteomyelitic symptoms. Implant coatings were well tolerated in bone. Further studies with additional control groups and longer time periods are warranted. Antibiotic-loaded phosphatidylcholine coatings applied at the point of care could prevent implant-associated infection in orthopaedic defects. | Jessica Amber Jennings Karen E Beenken Robert A Skinner Daniel G Meeker Mark S Smeltzer Warren O Haggard Karen S Troxel | 2016 | World Journal of Orthopedics2016，7，8： | 2 |
| 4 | Swamlata S, Cyclodextrin based novel drug delivery systems 显示文摘 | Amber V Shailendra S | 2008 | J Incl Phenom Macrocycl Chem2008，62，： | 1 |
| 5 | Sawdust: a green and economical sorbent for thallium removal显示文摘 | SAIMA Q M NAJMA M AMBER R S | 2008 | Chemical Engineering Journal2008，140，123： | 1 |
| 6 | Climate-induced boreal forest change: Predictions versus current observations显示文摘 | Amber J S Nadezda M T Nancy H F | 2007 | Global and Planetary Change2007，56，： | 1 |
| 7 | Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape 显示文摘 | Amber L J Hwajin K Divya S etal | | Proc Natl Acad Sci U S A0，，： | 1 |
| 8 | Human telomere disease due to disruption of the CCAAT box of the TERC promoter 显示文摘 | AMbers AM Kajigaya S van den Heuvel-Eibrink MM | 2012 | Blood2012，119，13： | 1 |
| 9 | Early nutrition tafter surgical treatment of gut perforations: A prospective randomized study 显示文摘 | Malhotra Amber Mathur AK Gupta S | 2004 | J Postgrad Med2004，50，2： | 1 |
| 10 | Sawdust: A green and economical sorbent for thallium removal 显示文摘 | Salma Q M Najma M Amber R S | 2008 | Chemical Engineering Journal2008，140，13： | 1 |
| 11 | Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity显示文摘 | amber M Flfickiger-Isler S Engelhardt G | 2009 | Mutagenesis2009，24，4： | 1 |
| 12 | Search for evidence of impact at the Permian -Triassic boundary in Antarctica and Australia显示文摘 | Retallack G J Seyodolali A Krull E S Holser W T Ambers C P Kyte F T | 1998 | Geology1998，26，： | 1 |
| 13 | Androgentie alopecia 显示文摘 | Amber ST Justin S | 2010 | J Mens Health2010，7，3： | 1 |
| 14 | Chemical composition and potential application of Spirulina platensis biomass显示文摘 | Aly M S Amber S Gad | | 0，，09： | 1 |
| 15 | Catechin degradation with concurrent formation of homo-and heterocatechin dimers during in vitro digestion显示文摘 | Andrew P N Amber S H Bruce R C | 2007 | Journal of Agricultural and Food Chemistry2007，55，22： | 1 |
| 16 | Androgenic alopecia显示文摘 | Amber ST Justin S | 2010 | J Mens Health2010，7，3： | 1 |
| 17 | High efficiency GaN-based LEDs and lasers on SiC显示文摘 | JOHN E AMBER A MIKE B JAYESH B KRISTIN L B DAVE E KEVIN H JAMES I MICHAEL L PHIL R DAVID S | 2004 | Journal of Crystal Growth2004，272，14： | 1 |
| 18 | A new spermidine alkaloid from Capparis decidua 显示文摘 | AHMAD V U ARIF S AMBER A U | 1985 | Heterocycles1985，23，12： | 1 |
| 19 | Invasive solid carcinoma of the breast: a report of two cases显示文摘 | Leena JB Kini RG Amber S | 2013 | J Clin Diagn Res2013，7，6： | 1 |
| 20 | A new alkaloid from root bark of Capparis decidua 显示文摘 | AHMAD V U ARIF S AMBER A U | 1986 | Org Chem1986，41，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员