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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | Characterization of two rat models of cystic fibrosis—KO and F508del CFTR—Generated by Crispr-Cas9显示文摘Background: Genetically engineered animals are essential for gaining a proper understanding of the disease mechanisms of cystic fibrosis(CF). The rat is a relevant laboratory model for CF because of its zootechnical capacity, size, and airway characteristics, including the presence of submucosal glands.Methods: We describe the generation of a CF rat model(F508 del) homozygous for the p.Phe508 del mutation in the transmembrane conductance regulator(Cftr) gene. This model was compared to new Cftr-/-rats(CFTR KO). Target organs in CF were examined by histological staining of tissue sections and tooth enamel was quantified by micro-computed tomography. The activity of CFTR was evaluated by nasal potential difference(NPD) and short-circuit current measurements. The effect of VX-809 and VX-770 was analyzed on nasal epithelial primary cell cultures from F508 del rats.Results: Both newborn F508 del and Knock out(KO) animals developed intestinal obstruction that could be partly compensated by special diet combined with an osmotic laxative. The two rat models exhibited CF phenotypic anomalies such as vas deferens agenesis and tooth enamel defects. Histology of the intestine, pancreas, liver, and lungs was normal. Absence of CFTR function in KO rats was confirmed ex vivo by short-circuit current measurements on colon mucosae and in vivo by NPD, whereas residual CFTR activity was observed in F508 del rats. Exposure of F508 del CFTR nasal primary cultures to a combination of VX-809 and VX-770 improved CFTR-mediated Cl-transport.Conclusions: The F508 del rats reproduce the phenotypes observed in CFTR KO animals and represent a novel resource to advance the development of CF therapeutics. | Elise Dreano Marc Bacchetta Juliette Simonin Louise Galmiche Claire Usal Lotfi Slimani Jérémy Sadoine Laurent Tesson Ignacio Anegon Jean-Paul Concordet Aurélie Hatton Lucile Vignaud Danielle Tondelier Isabelle Sermet-Gaudelus Marc Chanson Charles-Henry Cottart | 2019 | Animal Models and Experimental Medicine2019，2，4： | 3 |
| 3 | Chemistry in Spain:Bibiliomet-ric analysis through Scopus显示文摘 | Akvarez E C Anegon F D M | | 0，，06： | 1 |
| 4 | Presence of leukaemia inhibitory factor and interleukin 6in porcine secretion prior to onceptus attachment显示文摘 | Anegon I | 1994 | Cytokine1994，6，5： | 1 |
| 5 | Heme oxygenase and carbon monoxide as an immunotherapeutic approach in transplanta- tion and cancer显示文摘 | Simon T Anegon I Blancou P | 2011 | Immunotherapy2011，3，4： | 1 |
| 6 | CD8 regulatory T cells in solid organ transplantation显示文摘 | Guillonneau C Picarda E Anegon I | 2010 | Curr Opin Organ Transplant2010，15，6： | 1 |
| 7 | Data mining in a closed web environment 显示文摘 | Faba - Perez C Guerrero - Bote V P De Moya - Anegon F | 2003 | Seientometries2003，58，3： | 1 |
| 8 | Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells显示文摘 | Duluc D Delneste Y Tan F Moles M P Grimaud L Lenoir J Preisser L Anegon I Catala L Ifrah N Descamps P Gamelin E Gascan H Hebbar M Jeannin P | | 0，，13： | 1 |
| 9 | Over-expression of heme oxygenase-1 by adenoviral gene transfer improvers pregnancy outcom in a murine mode of abortion显示文摘 | Zenclusen M L Anegon I Bertoja A Z | 2006 | J Peprod Immunol2006，69，1： | 1 |
| 10 | A Connectionist and Multivariate Approach to Science Maps: The SOM, Clustering and MDS Applied to Library and Information Science Research 显示文摘 | Moya - Anegon F Herrero -Solana V Jimenez - Contreras E | 2006 | Journal of Information Science2006，32，1： | 1 |
| 11 | Over-expression of heine oxygenase 1 by adenoviral gene transfer improvers pregnancy outcom in a murine mode of abortion显示文摘 | Zenelussen ML Anegon I Bertoja AZ | 2006 | J Peprod Immunol2006，69，1： | 1 |
| 12 | Application of Gene Transfer Technologies to Transplantation显示文摘 | Ignacio Anegon Carole Guillonneau Marcelo Hill | 2004 | Ther Drug Monit · Volume2004，26，3： | 1 |
| 13 | Adenovirus-mediated expression of human CD55 or CD59 protects adult porcine islets from complement-mediated cell lysis by human serum显示文摘 | Goto M Le M B Anegon I Korsgren O | 2003 | Transplantation2003，75，5： | 1 |
| 14 | Safety and effectiveness of replacing standard doxazosin with doxazosin in the gastrointestinal therapeutic system (GITS) formulation in elderly bypertensive patients显示文摘 | JIMENEZ-GARCIA R ANEGON M ESTEBAN J | 2003 | Int J Clin Pract2003，57，4： | 1 |
| 15 | 显示文摘 | Anegon Ⅰ Cuturi MC Godard A | 1994 | Cytokine1994，6，5： | 1 |
| 16 | A test of genetic algorithms in relevance feedback 显示文摘 | Cristina Lopez - Pujalte Vicente P Guerrero Bote Felix de Moya - Anegon | 2002 | Information Processing and Management2002，38，： | 1 |
| 17 | Genetic algorithms in relevance feed- back:a second test and new contributions 显示文摘 | Cristina Lopez - Pujalte Vieente P Guerrero - Bote Felix de Moya- Anegon | 2003 | Information Processing and Management2003，39，： | 1 |
| 18 | A New Technique for Building Maps of large Seientific Domains Based on the Cocitation of Classes end Categories显示文摘 | FeLIX MOYA - ANEGoN BENJAMIN VARGAS - QUESADA VICTOR HERRERO - SOLANA etc | 2004 | Seientometries2004，61，1： | 1 |
| 19 | Safety and effectiveness of replacing standard doxazosin with doxazosin in the gastrointestinal therape utic system (GITS) formulation in elderly hypertensive patients 显示文摘 | Jimenez-Garcia R Anegon M Esteban J | 2003 | Int J Clin Pract2003，57，4： | 1 |
| 20 | Research fronts in library and information science in Spain (1985-1994) 显示文摘 | Anegon F D Contreras E J Corrochano M D | 1998 | Scientometrics1998，42，2： | 1 |
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