| 1 | A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell's transformation显示文摘A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G 2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis. | GAOYanhong QINLipeng ZHANGPeng CHENLin YUANHongfeng BAICixian YANFang YUEWen PEIXuetao | 2004 | Progress in Natural Science:Materials International2004，14，11： | 1 |
| 2 | Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro显示文摘Ascorbic acid (AA) induced differentiation of neural stem cells (NSCs) into dopaminergic (DAergic) neurons is reported. NSCs derived from rat mesencephalon were maintained and expanded in a defined medium containing mitogens of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Compared with the control, ascorbic acid treatment led to more DAergic neuronal differentiation as indicated by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT), which are specific markers of dopamine neurons. AA induction also enhanced expression of Nurr1 and Shh. PD98059, an inhibitor of mitogen activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway, could block AA induced Nurr1, TH and DAT mRNA expression. The results might suggest a new strategy to provide enough dopaminergic cells for the therapy of Parkinson’s disease (PD), and Nurr1 and ERK signaling pathway might participate in the AA induced DAergic differentiation. | ZHENGMin WANGDongmei HOULingling LIHaimin XIEChao JIAOWencang BAICixian WANGYaping PEIXuetao | 2004 | Progress in Natural Science:Materials International2004，14，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员