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| 1 | Differentiation of bone mar-row derived Thy-1^+β_2M^- cells into hepatocytes induced by coculture with transgenic CFSCs显示文摘Studies of transplantation in vivo indicted that bone marrow derived stem cells had a potential to different-tiate into mature hepatocytes. However, there are lots of doubts and uncertainties in the influencing factors and con-trol agents of effectively inducing stem cell differentiation in vitro, the efficiency of stem cells?differentiation into hepato-cytes and differentiated cells?life-span and functional state, etc. In this study, rat bone marrow derived Thy-1+β2M- cells (BDTCs) were induced to differentiate into hepatocytes by co-culturing with CFSC/HGF feeder layers which ex-pressed hHGF efficiently and stably. RT-PCR and im-munofluorescent texts proved induced BDTCs expressed infant and adult hepatocyte specific genes. Further more, these cells displayed functions of indocyanine green (ICG) uptake, ammonium metabolism and albumin production. It was shown that growth factors together with hepatic non-parenchyma cells provided a feasible microenvironment for differentiation of bone marrow stem cells into hepatocytes. The studies not only provided a significant biological model for going deep into the mechanism of stem cell plasticity, but also offered a theoretical and technical foundation of gene and stem cell engineering-based regenerative medicine for end-stage liver diseases. | WANGYunfang NANXue ZHANGRui LIYanhua YUEWen YAN-Fang PEIXuetao | 2004 | Chinese Science Bulletin2004，49，9： | 3 |
| 2 | A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell's transformation显示文摘A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G 2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis. | GAOYanhong QINLipeng ZHANGPeng CHENLin YUANHongfeng BAICixian YANFang YUEWen PEIXuetao | 2004 | Progress in Natural Science:Materials International2004，14，11： | 1 |
| 3 | In vitro induced dopaminergic differentiation of expanded rat mesencephalic neural stem cell显示文摘Neural stem cell (NSC) is the progenitor of the neural system with the character of self-renew and hav-ing the potential to differentiate into all the phenotypes in the central nervous system (CNS). NSC may serve as a source of cell transplantation for the treatment of neurodegenerative diseases to replace degenerative neurons. In this study, NSCs derived from E12.5 rat mesencephalon were main-tained and expanded using a serum-free defined medium containing basic fibroblast growth factor (bFGF) and epi-dermal growth factor (EGF). While proliferating, the cells were immunoreactive for nestin and remained multipotent to generate neurons, astrocytes, and oligodendrocytes. After 15 times passage the total number of the cell expanded about 2.4×104 fold. Compared with untreated cultures, ascorbic acid (AA) treatment led to more dopaminergic (DAergic) differentitiation as indicated by the expression of tyrosine hydroxylase (TH). With the concentration increasing, more TH+ neurons were obtained. 100 mmol/L AA could lead to a increase more than 20-fold, and a concentration of 10 靘ol/L could lead to nearly 5-fold increase in TH+ cells. However, the ratio of TH+ cells was not improved any longer with the AA increasing above the concentration of 100 靘ol/L. The results demonstrate that expanded NSCs can be induced to differentiate into dopamine neurons in vitro, which can pro-vide enough cell population for the cell transplantation, as a main intervention for the neurodegenerative diseases such as Parkinson抯 disease. | ZHENGMin WANGDongmei JIAOWenchang LIHaiming ZHAOLianxu BAIChixian WANGYaping PEIXuetao | 2003 | Chinese Science Bulletin2003，48，16： | 0 |
| 4 | Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro显示文摘Ascorbic acid (AA) induced differentiation of neural stem cells (NSCs) into dopaminergic (DAergic) neurons is reported. NSCs derived from rat mesencephalon were maintained and expanded in a defined medium containing mitogens of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Compared with the control, ascorbic acid treatment led to more DAergic neuronal differentiation as indicated by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT), which are specific markers of dopamine neurons. AA induction also enhanced expression of Nurr1 and Shh. PD98059, an inhibitor of mitogen activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway, could block AA induced Nurr1, TH and DAT mRNA expression. The results might suggest a new strategy to provide enough dopaminergic cells for the therapy of Parkinson’s disease (PD), and Nurr1 and ERK signaling pathway might participate in the AA induced DAergic differentiation. | ZHENGMin WANGDongmei HOULingling LIHaimin XIEChao JIAOWencang BAICixian WANGYaping PEIXuetao | 2004 | Progress in Natural Science:Materials International2004，14，1： | 0 |
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