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| 1 | 用酵母双杂交体系筛选与FMRP相作用蛋白的cDNA显示文摘目的采用酵母双杂交体系寻找与脆性X智力低下蛋白(Fragile X men-tal retadation protein,FMRP)相互作用的蛋白,探讨FMRP的生物医学功能。方法以编码FMRP_(Pro327-Thr518)肽段的cDNA序列与pBTM116质粒DNA结合结构域重组作为'钓饵',从小鼠胚胎cDNA文库中筛选与之相互作用蛋白的cDNA。结果筛选出13个与FMRP_(Pro327-Thr518)肽段特异性地相互作用的克隆,其中12个为小鼠泛素转运酶9(UBC9),1个是在GenBank中无高同源序列的未知cDNA序列。结论小鼠UBC9与人UBC9的氨基酸序列完全相同,且FMRP与UBC9蛋白表达的时空特征相似,因此认为人UBC9是与FMRP相互作用的蛋白,其相互作用的生物学意义有待进一步研究。本工作表明酵母双杂交体系是研究蛋白质相互作用的有效方法。 | 陈雨亭 Anne Sittler 余珉 Barbara Bardoni 吴冠芸 Jean Louis Mandel 沈岩 | 1998 | 中国医学科学院学报1998，20，3： | 4 |
| 2 | MicroRNAs as emerging biomarkers and therapeutic targets for pancreatic cancer显示文摘Despite tremendous efforts from scientists and clinicians worldwide, pancreatic adenocarcinoma(PDAC) remains a deadly disease due to the lack of early diagnostic tools and reliable therapeutic approaches. Consequently, a majority of patients(80%) display an advanced disease that results in a low resection rate leading to an overall median survival of less than 6 months. Accordingly, robust markers for the early diagnosis and prognosis of pancreatic cancer, or markers indicative of survival and/or metastatic disease are des-perately needed to help alleviate the dismal prognosis of this cancer. In addition, the discovery of new therapeutic targets is mandatory to design effective treatments. In this review, we will highlight the translational studies demonstrating that microRNAs may soon translate into clinical applications as long-awaited screening tools and therapeutic targets for PDAC. | Marion Gayral Sébastien Jo Naima Hanoun Alix Vignolle-Vidoni Hubert Lulka Yannick Delpu Aline Meulle Marlène Dufresne Marine Humeau Maёl Chalret du Rieu Barbara Bournet Janick Sèlves Rosine Guimbaud Nicolas Carrère Louis Buscail Jérome Torrisani Pierre Cordelier | 2014 | World Journal of Gastroenterology2014，20，32： | 4 |
| 3 | Role of endoscopic ultrasound in the molecular diagnosis of pancreatic cancer显示文摘Pancreatic ductal adenocarcinoma remains one of the most deadly types of tumor. Endoscopic ultrasoundguided fine-needle aspiration(EUS-FNA) is a safe, cost-effective, and accurate technique for evaluating and staging pancreatic tumors. However, EUS-FNA may be inconclusive or doubtful in up to 20% of cases. This review underlines the clinical interest of the molecular analysis of samples obtained by EUS-FNA in assessing diagnosis or prognosis of pancreatic cancer, especially in locally advanced tumors. On EUS-FNA materials DNA, mRNA and miRNA can be extracted, amplified, quantified and subjected to methylation assay. Kras mutation assay, improves diagnosis of pancreatic cancer. When facing to clinical and radiological presentations of pseudo-tumorous chronic pancreatitis, wildtype Kras is evocative of benignity. Conversely, in front of a pancreatic mass suspected of malignancy, a mutated Kras is highly evocative of pancreatic adenocarci-noma. This strategy can reduce false-negative diagnoses, avoids the delay of making decisions and reduces loss of surgical resectability. Similar approaches are conducted using analysis of miRNA expression as well as Mucin or markers of invasion(S100P, S100A6, PLAT or PLAU). Beyond the diagnosis approach, the prediction of response to treatment can be also investigated form biomarkers expression within EUS-FNA materials. | Barbara Bournet Marion Gayral Jérme Torrisani Janick Selves Pierre Cordelier Louis Buscail | 2014 | World Journal of Gastroenterology2014，20，31： | 3 |
| 4 | Interventional Endoscopic Ultrasound in Pancreatic Diseases显示文摘 | Louis Buscail Patrick Faure Barbara Bournet Janick Selves Jean Escourrou | 2006 | Pancreatology . 2006 (1-2)2006，，： | 1 |
| 5 | Mechanisms of Trypanosoma cruzi persistence in Chagas disease显示文摘 | FnuNagajyothi Fabiana S.Machado Barbara A.Burleigh Linda A.Jelicks Philipp E.Scherer ShankarMukherjee Michael P.Lisanti Louis M.Weiss Nisha J.Garg Herbert B.Tanowitz | 2012 | Cellular Microbiology2012，，5： | 1 |
| 6 | Optic nerve head (ONH) topographic analysis by stratus OCT in normal subjects: correlation to disc size, age, and ethnicity 显示文摘 | Barbara C Marsh B Louis B Hoop J Lipyanik J Patella VM | 2010 | J Glaucoma2010，19，5： | 1 |
| 7 | Epidermal growth factor primes intestinal epithelial cells for proliferative effect of insulin-like growth factor I显示文摘 | Mark D. Duncan MD Louis Y. Korman MD Dr. Barbara L. Bass MD | 1994 | Digestive Diseases and Sciences1994，，10： | 1 |
| 8 | Biventricular Pacing in Severe Heart Failure Patients Reverses Electromechanical Dyssynchronization from Apex to Base显示文摘 | JEAN‐FRAN?OIS TOUSSAINT THOMAS LAVERGNE JACKY OLLITRAUT CHANTAL HIGNETTE JEAN‐MARC DARONDEL BARBARA DIEULEVEULT MARC FROISSART JEAN‐YVES LE HEUZEY LOUIS GUIZE MICHEL PAILLARD | 2000 | Pacing and Clinical Electrophysiology . 2000 (11P2)2000，，11： | 1 |
| 9 | The use of functional electrical stimulation cycles in children and adolescents with spinal cord dysfunction:a pilot study显示文摘 | Frank Castello Barbara Louis JenFu Cheng Michael | 2012 | Journal of pediatric rehabilitation medicine2012，5，4： | 1 |
| 10 | Outcomes of nonresected main-duct intraductal papillary mucinous neoplasms of the pancreas显示文摘AIM:To compare characteristics and outcomes of resected and nonresected main-duct and mixed intraductal papillary mucinous neoplasms of the pancreas(IPMN).METHODS:Over a 14-year period,50 patients who did not undergo surgery for resectable main-duct or mixed IPMN,for reasons of precluding comorbidities,age and/or refusal,were compared with 74 patients who underwent resection to assess differences in rates of survival,recurrence/occurrence of malignancy,and prognostic factors.All study participants had dilatation of the main pancreatic duct by ≥ 5 mm,with or without dilatation of the branch ducts.Some of the nonsurgical patients showed evidence of mucus upon perendoscopic retrograde cholangiopancreatography or endoscopic ultrasound and/or after fine needle aspiration.For the surgical patients,pathologic analysis of resected specimens confirmed a diagnosis of IPMN with involvement of the main pancreatic duct or of both branch ducts as well as the main pancreatic duct.Clinical and biologic follow-ups were conducted for all patients at least annually,through hospitalization or consultation every six months during the first year of follow-up,together with abdominal imaging analysis(magnetic resonance cholangiopancreatography or computed tomography) and,if necessary,endoscopic ultrasound with or without fine needle aspiration.RESULTS:The overall five-year survival rate of patients who underwent resection was significantly greater than that for the nonsurgical patients(74% vs 58%; P =0.019).The parameters of age(< 70 years) and absence of a nodule were associated with better survival(P < 0.05); however,the parameters of main pancreatic duct diameter > 10 mm,branch ductdiameter > 30 mm,or presence of extra pancreatic cancers did not significantly influence the prognosis.In the nonsurgical patients,pancreatic malignancy occurred in 36% of cases within a mean time of 33 mo(median:29 mo; range:8-141 mo).Comparison of the nonsurgical patients who experienced disease progression with those who did not progress showed no significant differences in age,sex,symptoms,subtype of IPMN,or follow-up period; only the size of the main pancreatic duct was significantly different between these two sub-groups,with the nonsurgical patients who experienced progression showing a greater diameter at the time of diagnosis(> 10 mm).CONCLUSION:Patients unfit for surgery have a 36% greater risk of developing pancreatic malignancy of the main-duct or mixed IPMN within a median of 2.5 years. | Mathieu Daudé Fabrice Muscari Camille Buscail Nicolas Carrère Philippe Otal Janick Selves Louis Buscail Barbara Bournet | 2015 | World Journal of Gastroenterology2015，21，9： | 0 |
| 11 | 新英格兰医学中心后循环登记显示文摘在407例新英格兰医学中心后循环登记患者中,59%为不伴TIA的卒中,24%在卒中前有TIA,16%仅表现为TIA。栓塞是最常见的卒中机制(占40%,其中24%为心源性栓塞,14%为动脉-动脉栓塞,2%两者兼有)。在32%的患者中,大动脉闭塞性损害导致血流动力学性脑缺血。梗死最常出现在后循环远段供血区(脑干腹侧、小脑上部以及枕叶和颞叶),近段(延髓和小脑后下部)和中段(桥脑和小脑前下部)供血区受累的比例相当。148例患者存在至少累及1支大动脉的重度闭塞性损害(狭窄〉50%),134例患者有单侧或双侧动脉受累。最常见的闭塞部位是椎动脉颅外段(52例患者,15例双侧)、椎动脉颅内段(40例患者,12例双侧)和基底动脉(46例患者)。动脉-动脉栓塞是椎动脉闭塞性疾病患者最常见的脑梗死发病机制。30d病死率为3.6%。发病机制为栓塞,远段供血区受累和基底动脉闭塞性疾病患者的预后最差。具有多处动脉狭窄部位的患者转归最好。他们通常经年累月地发生TIA。 | Louis R. Caplan Robert J. Wityk Thomas A. Glass Jorge Tapia Ladislav Pazdera Hui-Meng Chang Phillip Teal John F. Dashe Claudia J. Chaves Joan C. Breen Kostas Vemmos Pierre Amarenco Barbara Tettenborn Megan Leary Conrad Estol, L Dana Dewitt Michael S. Pessin 程言博(校) 刘春风(校) | 2005 | 中华脑血管病论坛2005，3，2： | 0 |
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