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| 1 | Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR显示文摘AIM:To investigate three isoforms of survivin in colorectal adenocarcinomas.METHODS:We used the LightCycler Technology(Roche),along with a common forward primer and reverse primers specific for the splice variants and two common hybridization probes labeled with fluorescein and LightCycler-Red fluorophore(LC-Red 640).Real time quantitative polymerase chain reaction(PCR) was performed on cDNAs from 52 tumor specimens from colorectal cancer patients and 10 unrelated normal colorectal tissues.In the patients group,carcinoembryonic antigen(CEA) and CA19-9 tumor markers were also measured immunochemically.RESULTS:Wild type survivin mRNA isoform was expressed in 48%of the 52 tumor samples,survivin-2b in 38%and survivin-ΔΕx3 in 29%,while no expression was found in normal tissues.The mRNA expression of wild type survivin presented a significant correlation with the expression of the ratio of survivin-2b,survivin-ΔΕx3,survivin-2b/wild type survivin and survivin-ΔΕx3/wild type survivin(P<0.001).The mRNA expression of wildsurvivin and survivin-ΔΕx3 was related with tumor size and invasion(P=0.006 and P<0.005,respectively).A significant difference was found between survivin-2b and morphologic cancer type.Also,the ratio of survivin-ΔEx3/ wild-survivin was significantly associated with prognosis.No association was observed between the three isoforms and grade,metastasis,Dukes stage and gender.The three isoforms were not correlated with CEA and CA19-9.CONCLUSION:Survivin isoforms may play a role in cell apoptosis and their quantification could provide information about clinical management of patients suffering from colorectal cancer. | Anastasia Pavlidou Maria Dalamaga Christos Kroupis George Konstantoudakis Maria Belimezi George Athanasas Kleanthi Dimas | 2011 | World Journal of Gastroenterology2011，17，12： | 9 |
| 2 | Autoantibodies to lipoprotein-related protein 4 in patients with double-seronegative myasthenia gravis显示文摘 | Zhang B Tzartos JS Belimezi M | 2012 | Arch Neurol2012，69，4： | 1 |
| 3 | Autoantibodies to lipopro- tein-related protein 4 in patients with double-seronegative myasthe- nia gravis 显示文摘 | Zhang B Tzartos JS Belimezi M | 2012 | Arch Neural2012，69，4： | 1 |
| 4 | Growth inhibition of breast cancer cell lines overexpressing Her2/neu by a novel internalized fully human Fab antibody fragment显示文摘 | Belimezi MM Papanastassiou D Merkouri E | 2006 | Cancer Immunol Immunother2006，55，9： | 1 |
| 5 | Autoantibodies to lipoprotein-related protein 4 in patients with double-seronegative myasthenia gravis显示文摘 | Zhang B Tzartos JS Belimezi M | 2012 | Arch Neurol2012，69，4： | 1 |
| 6 | Autoantibodies to lipoprotein-related protein 4 in patients with doubleseronegative myasthenia gravis显示文摘 | Zhang B Tzartos J S Belimezi M | 2012 | Arch Neurol2012，69，4： | 1 |
| 7 | Growth inhibition of breast cancer cell lines over expressing Her2/neu by a novel internalized fully human Fab antibody fragment显示文摘 | Maria M Belimezi Danai Papanastassiou | 2005 | Cancer Immunol Immunothe2005，262，： | 1 |
| 8 | Autoantibodies to lipoprotein-related protein 4 in patients with double-semnegative myasthenia gravis显示文摘 | Zhang B Tzartos J S Belimezi M | 2012 | Arch Neurol2012，69，4： | 1 |
| 9 | Autoantibodies to Lipoprotein-Related Protein 4 in Patients With Double-Seronegative Myas- thenia Gravis 显示文摘 | Zhang B Tzartos JS Belimezi M | 2012 | Arch Neurol2012，69，4： | 1 |
| 10 | Autoantibodies to lipoprotein- related protein 4 in patients with double-seronegative myasthenia gra- vis显示文摘 | Zhang B Tzartos JS Belimezi M | 2012 | Archives of Neurology2012，69，4： | 1 |
| 11 | Growth inhibition of breast cancer cell lines overexpressing Her2/neu by a novel internalized fully human Fab antibody fragment显示文摘 | Belimezi M M Papan astassiou D Merkouri E | 2006 | Cancer Immunol Immnunother2006，55，9： | 1 |
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