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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Vascular endothelial growth factor receptor 2(VEGFR-2) plays a key role in vasculogenic mimicry formation,neovascularization and tumor initiation by Glioma stem-like cells显示文摘Human glioblastomas(GBM)are thought to be initiated by glioma stem-like cells(GSLCs).GSLCs also participate in tumor neovascularization by transdifferentiating into vascular endothelial cells.Here,we report a critical role of GSLCs in the formation of vasculogenic mimicry(VM),which defines channels lined by tumor cells to supply nutrients to early growing tumors and | Yao X Ping Y Liu Y Chen K Yoshimura T Liu M Gong W Chen C Niu Q Guo D Zhang X Wang JM Bian X | 2013 | 中国神经肿瘤杂志2013，11，3： | 23 |
| 3 | Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats显示文摘AIM:To investigate the key factors in developing the trinitrobenzene sulfonic acid(TNBS)-induced postinflammatory irritable bowel syndrome(PI-IBS)model in rats. METHODS:TNBS was administered to rats at the following conditions:(1)with different doses(20,10,5 mg/0.8 mL per rat);(2)with same dose in different concentrations(20 mg/rat,25,50 mg/mL);(3)in different ethanol percentage(25%,50%);and(4)at depth either 4 cm or 8 cm from anus.At 5 d and 4 wk after TNBS administration,inflammation severity and inflammation resolution were evaluated.At 4 and 8 wk after TNBS application,visceral hyperalgesia and enterochromaffin(EC)cell hyperplasia were assayed by abdominal withdrawal reflex test,silver staining and capillary electrophoresis. RESULTS:Our results showed that:(1)TNBS induced dose-dependent acute inflammation and inflammation resolution.At 5 d post TNBS,the pathological score and myeloperoxidase(MPO)activity in all TNBS treated rats were significantly elevated compared to that of the control(9.48±1.86,8.18±0.67,5.78± 0.77 vs 0,and 3.55±1.11,1.80±0.82,0.97±0.08 unit/mg vs 0.14±0.01 unit/mg,P<0.05).At 4 wk post TNBS,the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control(1.52±0.38 and 0.80±0.35 vs 0,P<0.05);(2)Intracolonic TNBS administration position affected the persistence of visceral hyperalgesia.At 4 wk post TNBS,abdominal withdrawal reflex (AWR)threshold pressure in all TNBS-treated groups were decreased compared to that of the control(21.52 ±1.73 and 27.10±1.94 mmHg vs 34.44±1.89 mmHg,P<0.05).At 8 wk post TNBS,AWR threshold pressure in 8 cm administration group was still significantly decreased(23.33±1.33 mmHg vs 36.79±2.29 mmHg,P<0.05);(3)Ethanol percentage affected the TNBS-induced inflammation severity and visceral hyperalgesia.In TNBS-25%ethanol-treated group,the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50%ethanoltreated group,while AWR threshold pressure were significantly elevated(36.33±0.61 mmHg vs 23.33±1.33 mmHg,P<0.05);and(4)TNBS(5 mg/0.8 mL per rat, in 50%ethanol,8 cm from anus)-treated rats recovered completely from the inflammation with acquired visceral hyperalgesia and EC cell hyperplasia at 4 wk after TNBS administration.CONCLUSION:TNBS dosage,concentration,intraco-lonic administration position,and ethanol percentage play important roles in developing visceral hyperalgesia and EC cell hyperplasia of TNBS-induced PI-IBS rats. | Hong-Yan Qin Hai-Tao Xiao Justin CY Wu Brian M Berman Joseph JY Sung Zhao-Xiang Bian | 2012 | World Journal of Gastroenterology2012，18，20： | 10 |
| 4 | 母乳喂养和孕产妇心血管病风险:30万中国妇女的前瞻性研究显示文摘母乳喂养给儿童健康带来了大量好处,并且也与母亲生命后期心血管病风险降低相关。然而,有关其对心血管病影响的证据仍然不一致,特别是在东亚人中,母乳喂养的频率和持续时间与西方国家的频率和持续时间显著不同。2004-2008年,中国香港嘉道理生物样本库(China Kadoorie biobank)从中国10个不同地区招募了年龄30-79岁受试者50万人,基线无心血管病的女性受试者289 573人在8年的随访中, | 刘莉 叶鹏 Peters SAE Yang L Guo Y Chen Y Bian Z Du J Yang J Li S Li L Woodward M Chen Z | 2017 | 中华高血压杂志2017，25，8： | 4 |
| 5 | ComparativeStudy on Sorption/Adsorption of Radioeuropium onAlumina,Bentonite and Red Earth:Effects of pH,Ionic Strength,Fulvic Acid and Iron Oxids in RedEarth显示文摘 | Dong W M Wang X K Bian X Y | 2001 | Appl Radiat Isot2001，54，4： | 1 |
| 6 | Interfacial shear stresses concentration in CFRP - strengthened beams 显示文摘 | MAALEJ M BIAN Y | 2001 | Composite Structures2001，54，： | 1 |
| 7 | Immunochemical analysis of human papillomavirus L1 capsid protein in Liquid-based cytology samples from cervical lesions显示文摘 | Xiao W Bian M Ma L | 2012 | Acta Cytol2012，54，5： | 1 |
| 8 | Real-time monitoring of the strand dis- placement amplification(SDA) of human cytomegalovirus by a new SDA- piezoelectric DNA sensor system显示文摘 | Chert Q Bian Z Chen M | 2009 | Biosens Bioelectron2009，24，12： | 1 |
| 9 | Efficacy of bone marrowderived mesenchymal stem cells in the treatment of selerodermatous chronic graft-versus-host disease: clinical report显示文摘 | Zhou H Guo M Bian C | 2010 | Biol Blood and Marrow Transplant2010，16，3： | 1 |
| 10 | Efficacy of bone marrow- derived mesenchymal stem cells in the treatment of sclerodermatous chronic graft-versus-host disease: clinical report显示文摘 | Zhou H Guo M Bian C | 2010 | Biol Blood Marrow Transplant2010，16，3： | 1 |
| 11 | 查看详情显示文摘 | Bian J Xiao M Wang S J Lu Y X Meng Y Z | | 0，，： | 1 |
| 12 | Prevalence and trend of major transfusion - transmissible infections among blood donors in western China, 2005 through 2010 显示文摘 | SONG Y BIAN Y PETZOLD M | 2014 | PLoS One2014，9，4： | 1 |
| 13 | p16 inactivation by methylation of the CDKN2A promoter occurs early during neoplastic progression in Barrett's esophagus显示文摘 | Bian Y S Osterheld M C Fontolliet C | 2002 | Gastroenterology2002，122，4： | 1 |
| 14 | Ultrasensitive De- tection of Lead Ion Sensor Based on Gold Nanoden- drites Modified Electrode and Electrochemilumines- cent Quenching of Quantum Dots by Electrocatalytic Silver/Zinc Oxide Coupled Structures 显示文摘 | Li M Kong Q K Bian Z Q | 2014 | Biosen- sors and Bioelectronics2014，65，： | 1 |
| 15 | Molecular approaches identify known species, reveal cryptic species and verify host specificity of Chinese Philotrypesis (Hymenoptera: Pteromalidae)显示文摘 | Zhou M J Xiao JH Bian SN | 2012 | Mol Ecol Resour2012，12，4： | 1 |
| 16 | Sensitivity-enhanced reflection Z-scan by oblique incidence of a polarized beam显示文摘 | Martinelli M Bian S Leite J R | | 0，，： | 1 |
| 17 | The design of sustainable logistics network under uncertainty显示文摘 | Lee D H Dong M Bian W | | 0，，： | 1 |
| 18 | Novel application of thermally expanded graphite as the support of catalysts for direct synthesis of DMC from CH3OH and CO2 显示文摘 | BIAN J XIAO M WANG S J | 2009 | J Colloid Interface Sci2009，334，： | 1 |
| 19 | ,显示文摘 | Bian J M Li X M Gao X D | 2004 | Appl Phys Lett 84:2004，541，： | 1 |
| 20 | Typing of mutans Streptococci by arbitrarily primed PCR in patients undergoing orthodontic treatment显示文摘 | Liu J Bian Z Fan M | 2004 | Caries Res2004，38，6： | 1 |
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