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| 1 | The chemosensory protein of Chinese honeybee, Apis cerana cerana: Molecular cloning of cDNA, immunocytochemical localization and expression显示文摘Chemosensory proteins (CSPs) are ubiquitous soluble small proteins isolated from sensory organs thought to be involved in chemical communication. Here we report the first cDNA of CSPs, called Ac-ASP3, cloned and characterized from antennas of adult worker bees in Chinese honeybee, Apis cerana cerana. The Ac-ASP3 cDNA comprises 2 exons, with an ORF of 393-bp encoding 130 aa. Protein signature analyses show that the protein consists of four conserved cysteines and a signal peptide with 19 aa in the N-terminal sequence. The deduced protein sequence shares high homology with Am-ASP3 of Apis mellifera and low similarity with other species of insects. Immunocytochemical localization shows that Ac-ASP3 is only specifically expressed on the antenna contact chemosensilla such as sensilla trichodea B and sensilla basiconica, whereas Ac-ASP3 is scarcely expressed on olfactory chemosensilla such as sensilla placodea. Real-time PCR of Ac-ASP3 transcripts shows that Ac-ASP3 is highly expressed on wings and legs, but expression is lower on antenna. Temporal expression patterns suggest that Ac-ASP3 is expressed during the period of pupa and adults from 1-d to 6-d stages when bees act as house bees, cleaning the comb and taking care of the queen and larvae in comb. The above evidence suggests that Ac-ASP3 is unique in species and is generally not involved in olfaction during searching for honey and pollen. Rather, the protein seems to function in recognition of chemosensory substances on bees' cuticle and mechanical movement of antenna. | LI HongLiang LOU BingGan CHENG Jia'An GAO QiKang | 2007 | Chinese Science Bulletin2007，52，10： | 10 |
| 2 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China显示文摘 | Binggan Wei Linsheng Yang | 2009 | Microchemical Journal2009，，2： | 9 |
| 3 | Piriformospora indica confers cadmium tolerance in Nicotiana tabacum显示文摘Piriformospora indica, a root-colonizing endophytic fungus of Sebacinales, promotes plant growth and confers resistance against biotic and abiotic stresses. In order to confirm the influence of P. indica on growth, proline, malondialdehyde(MDA), chlorophyll, and cadmium(Cd) amounts in Nicotiana tabacum under Cd stress, hydroponics, pot and field trials were conducted. The results showed that P. indica can store Cd in plant roots and reduce leaf Cd content, reduce the concentration of MDA, and increase the proline and chlorophyll content and the activities of catalase, peroxidase, and superoxide dismutase under hydroponic Cd stress. RT-PCR analysis showed that the relative expression level of genes Gsh2, Ta PCS1, oas1, GPX, and Hsp70 in colonized plants was 4.3, 1.4, 2.9, 1.7, and 6.9fold higher than in un-colonized plants respectively. Cd exposure significantly reduced un-colonized plants' agronomic traits compared to P. indica-colonized ones. Our results suggested that P. indica can sequester Cd in roots, so that much less cadmium was transported to leaves, and the increased concentrations of antioxidant enzymes, pigments and proline contents, as well as the higher expression of stress-related phytochelatin biosynthesis genes in P. indica-inoculated plants, may also serve to protect N. tabacum plants against oxidative damage, enhancing Cd tolerance. | Feiqiong Hui Jian Liu Qikang Gao Binggan Lou | 2015 | Journal of Environmental Sciences2015，27，11： | 6 |
| 4 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China显示文摘 | Binggan Wei Linsheng Yang | 2009 | Microchemical Journal2009，，2： | 2 |
| 5 | A review of heavy metal con- taminations in urban soils, urban road dusts and agricultural soils from China 显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microchemical Journal2010，94，13： | 1 |
| 6 | A study of air pollutants influencing lifeexpectancy and longevity from spatial perspective in China显示文摘 | LI Wang WEI Binggan LI Yonghua LI Hairong ZHANG Fengying ROSENBERG Mark YANG Linsheng HUANG Jixia KRAFFTThomas WANG Wuyi | 2014 | Science ofthe Total Environment2014，487，15： | 1 |
| 7 | Spatial distribution and contamination assessment of heavy met- als in urban road dusts from Urumqi, NW China显示文摘 | Wei Binggan Jiang Fengqing Li Xuemei | 2009 | Microchemical Journal2009，93，2： | 1 |
| 8 | Impacts of land use on spatial distribution of mortalityrates of cancers caused by naturally occurring asbestos显示文摘 | WEI Binggan JIA Xianjie YANG Linsheng | 2012 | J Expo Sci Environ Epidemiol2012，22，5： | 1 |
| 9 | Spatial distribution and contamination assessment of heavy metals in urban road dusts from Urumqi,NW China显示文摘 | WEI Binggan JIANG Fengqing LI Xuemei | 2009 | Microchemical Journal2009，，： | 1 |
| 10 | A review of heavy metal contaminations in urb an soils, urban road dusts and agricultural soil from China显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microc hemical Journal2010，94，2： | 1 |
| 11 | Spatial dis- tribution and contamination assessment of heavy metals in ur- ban road dusts from Urumqi, NW China显示文摘 | WEI Binggan J]ANG Fengping LI Xuemei | 2009 | Microchemical Journal2009，93，2： | 1 |
| 12 | A review of heavy metal contaminations inurban soils,urban road dusts and agricultural soils fromChina显示文摘 | Binggan W Yang L | 2010 | Microchemical Journal2010，94，1： | 1 |
| 13 | A review of heavy metalcontaminations in urban soils, urban road dusts andagricultural soils from China Review Article 显示文摘 | Wei Binggan Yang Linsheng | 2010 | Micro-chem J2010，94，2： | 1 |
| 14 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microchemical Journal2010，94，2： | 1 |
| 15 | A review of heavy metal con taminations in urban soils, urban road dusts and agricultural soils from China 显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microchemical Journal2010，94，2： | 1 |
| 16 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China 显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microchemical Journal2010，94，2： | 1 |
| 17 | Rare earth elements in human hair from a mining area of China显示文摘 | Binggan Wei Yonghua Li Hairong Li Jiangping Yu Bixiong Ye Tao Liang | 2013 | Ecotoxicology and Environmental Safety2013，，： | 1 |
| 18 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China显示文摘 | Binggan Wei Linsheng Yang | 2009 | Microchemical Journal2009，，2： | 1 |
| 19 | Spatial distribution and contamination assessment of heavy metals in urban road dusts from Urumqi, NW China显示文摘 | Binggan Wei Fengqing Jiang Xuemei Li Shuyong Mu | 2009 | Microchemical Journal2009，，2： | 1 |
| 20 | A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China显示文摘 | WEI Binggan YANG Linsheng | 2010 | Microchem J2010，94，2： | 1 |
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