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| 1 | A RHOse by any other name:a comparative analysis of animal and plant Rho GTPases显示文摘Rho GTPases 是作为许多的关键管理者扮演细胞的进程的分子的开关,包括房间运动,形态发生,主机防卫,房间部门和基因表示。Rho GTPases 在所有真核细胞的王国被发现。植物缺乏清楚的相当或相同的事物到在酵母和动物发现的常规 Rho GTPases;相反,他们随着时间的过去开发了一个唯一的亚科, ROP,是也知道皇家装甲兵。象 ROP 一样蛋白质的起源看起来先于陆地植物的外观。这评论试图讨论 ROP/RAC 的进化并且比较植物 ROP 和动物 Rho GTPases,集中于 GTPases 和他们的下游的受动器的规定的类似和差别。 | Tore Brembu Per Winge Atle Magnar Bones Zhenbiao Yang | 2006 | Cell Research2006，16，5： | 7 |
| 2 | Stable isotope labeling of nanomaterials for biosafety evaluation and drug development显示文摘Quantitative information,such as environmental migration,absorption,biodistribution,biotransformation,and elimination,is fundamental and essential for the nanosafety evaluations of nanomaterials.Due to the complexity of biological and environmental systems,it is challenging to develop quantitative approaches and tools that could characterize intrinsic behaviors of nanomaterials in the organisms.The isotopic tracers are ideal candidates to tune the physical properties of nanomaterials while preserving their chemical properties.In this review article,we summarized the stable isotope labeling methods of nanomaterials for evaluating their environmental and biological effects.The skeleton labeling protocols of carbon nanomaterials and metal/metal oxide nanoparticles were introduced.The advantages and disadvantages of stable isotope labeling were discussed in comparison with other quantitative methods for nanomaterials.The quantitative information of nanomaterials in environmental and biological systems was summarized along with the biosafety data.The benefits for drug development of nanomedicine were analyzed based on the targeting effects,persistent accumulation,and safety.Finally,the challenges and future perspectives of stable isotope labeling in nanoscience and nanotechnology were discussed. | Xue-Ling Chang Lingyun Chen Boning Liu Sheng-Tao Yang Haifang Wang Aoneng Cao Chunying Chen | 2022 | Chinese Chemical Letters2022，33，7： | 2 |
| 3 | Effects of denosumab treatment and discontinuation on bone mineral density and bone turnover markers in postmenopausal women with low bone mass显示文摘 | Bone HG Bolognese MA Yuen CK Kendler DL Miller PD Yang YC Grazette L San Martin J Gallagher JC | | 0，，： | 1 |
| 4 | Changes in the proliferative activity of hippocampal neural stem cells from manganismus mice显示文摘BACKGROUND: Manganese neurotoxicity presents in the form of not only extracorticospinal tract injury of central nervous system (CNS), but also learning and memory ability damage. So, the mechanism of manganese neurotoxicity will be further studied from the angle of hippocampus. OBJECTIVE: To observe the effects of manganism on learning and memory ability and the proliferation of neural stem cells (NSCs) in hippocampus of mouse brains, and analyze whether this effect has dose-dependence. DESIGN: Randomized controlled experiment. SETTING: Department of Human Anatomy, and Department of Industrial Hygiene and Occupational Diseases, Guangxi Medical University. MATERIALS: Twenty-eight male Kunming mice, aged 2 weeks, were involved in this experiment. The involved mice were randomized into 4 groups, with 7 in each: control group, low-dose manganism group, middle-dose manganism group and high-dose manganism group. Manganese chloride was purchased from Shantou Chemicals Factory. METHODS: This experiment was carried out in the Experimental Center for Preclinical Medicine, Guangxi Medical University from November 2005 to August 2006. Mice in the low-, middle- and high-dose manganism groups were intraperitoneally injected with 5, 20 and 50 mg/kg per day manganese chloride, once a day, for 2 weeks successively. Mice in the control group were injected with the same amount of stroke-physiological saline solution. Neurobehavioral detection of all the animals was performed in Morris water maze constantly from the 7th day after the first injection of manganese chloride solution. Learning ability was detected in the place navigation test. Mice were trained for 5 consecutive days with four trials per day. The time to find the platform was latency. Memory ability was detected in spatial probe test. Platform was withdrawn on the following day of place navigation. The mice were placed in the water from a random start in the edge of the pool. The number of times they traversed the plateform's region was recorded as the performance of spatial memory. At the final two days of the water maze tests, all the animals were daily intraperitoneally injected with 50 mg/kg BrdU three times successively, once every 4 hours. At 24 hours after the final BrdU injection, all the animals were sacrificed and perfused, and their brains were harvested, fixed and successively sliced at coronary plane on a freezing microtome. Distribution and number of BrdU-positive cells in the subgranular zone of hippocampus of brains of experimental animas were detected respectively by immunohistochemistry for reflecting the proliferation of NSCs. Single-factor analysis of variance was used for comparing the difference of measurement data. Linear correlation analysis was used among the performance record in Morris water maze test, the number of BrdU-immunopositive cells and the dose of manganism. MAIN OUTCOME MEASURES: Learning and memory ability and the number of hippocampal NSCs of mice in each group. RESULTS: ①Performance of mice in Morris water maze: In the place navigation test, there was a significant retarded learning in mice of high-dose manganism group from the 3rd day as compared with control group (P < 0.01). Till the 5th day, escape latency of mice in each manganism group was prolonged, and learning performance was significantly decreased (P < 0.05), while swimming speed did not affect above results. In the spatial probe test, the average frequency of middle- and high-dose manganism groups was 1.17±1.60 and 0.80±1.10, respectively, and decreased remarkably than that of control group which was 4.86±1.35 (P < 0.01), indicating memory ability was decreased; while the average frequency of low-dose manganism group did not differ obviously from that of control group (P =0.066) although it was 2.67±3.27. The difference of swimming speed in each group was still of no statistic significance (P > 0.05). ②Effect ofmanganism on the number of NSCs: After counting, the average number of BrdU- immunopositive cells of one side in the control group, low-, middle- and high-dose manganism groups was 69.20±4.48, 36.63±4.50, 31.00±6.87, 26.76±4.83, individually (P < 0.01). ③Results of linear correlation analysis: The ability of spatial memory was significantly in positive correlation with the number of BrdU-labeled cells ( r =0.734, P < 0.01), and in negative correlation with the intensity of manganese poisoning (r =–0.598,P < 0.01).Meanwhile, there was a significant negative correlation between the number of BrdU-labeled cells and the intensity of manganese poisoning(r =–0.666, P < 0.01). CONCLUSION: Manganese exposure in mice can affect the ability of learning and memory, which is probably caused by the inhibition of manganese to the neurogenesis of NSCs in hippocampus in dose-dependent manner. | Guohe Tan Boning Yang Guofu Tan Bo Liang Jiangu Gong Xiaodong Ge Songchao Guo | 2007 | Neural Regeneration Research2007，2，4： | 1 |
| 5 | Improved genetic distance-based spatial deployment can effectively minimize inbreeding in seed orchard显示文摘Background:Inbreeding in seed orchards is expected to increase with the advancement of breeding cycles,which results in the delivery of crops with suboptimal genetic gain,reduced genetic diversity,and lower seed set.Here,a genetic distance-dependent method for clonal spatial deployment in seed orchards was developed and demonstrated,which reduced the inbreeding levels.The method’s main evaluation parameter of inbreeding is the genetic distance among individuals and the deployment method used an improved adaptive parallel genetic algorithm(IAPGA)based on Python language.Using inbreeding-prone Chinese Mongolian pine breeding population material originating from a single natural population,the proposed method was compared to a traditional orchard design and a distance-based design;namely,complete randomized block(RCB)and optimum neighborhood(ONA)designs,respectively.Results:With the advancement of selective breeding cycles,group separation among orchard related individuals is expected to increase.Based on the genetic distance among individuals,the IAPGA design was superior in significantly reducing the inbreeding level as compared to the two existing designs,confirming its suitability to advanced-generation orchards where relatedness among parents is common.In the 1st,2nd,and mixed generations clonal deployment schemes,the IAPGA design produced lower inbreeding with 87.22%,81.49%,and 87.23%of RCB,and 92.78%,91.30%,and 91.67%of ONA designs,respectively.Conclusions:The IAPGA clonal deployment proposed in this study has the obvious advantage of controlling inbreeding,and it is expected to be used in clonal deployment in seed orchards on a large-scale.Further studies are needed to focus on the actual states of pollen dispersal and mating in seed orchards,and more assumptions should be taken into account for the optimized deployment method. | Boning Yang Haihong Sun Jiandong Qi Shihui Niu Yousry AEl-Kassaby Wei Li | 2020 | Forest Ecosystems2020，7，1： | 1 |
| 6 | Single-cell transcriptomic analysis uncovers intratumoral heterogeneity and drug-tolerant persister in ALK-rearranged lung adenocarcinoma显示文摘Dear Editor,Non-small cell lung carcinoma(NSCLC)accounts for more than 85%of all lung cancers[1].Lung adenocarcinomas(ADCs)arising from never-smokers have been frequently found to be associated with oncogenic driver mutations,including anaplastic lymphoma kinase(ALK)rearrangement.Echinoderm microtubule-associated protein-like 4(EML4)is the most common fusion partner of the ALK gene[2].Tyrosine kinase inhibitors(TKIs)are currently the first-line treatment for advanced EML4-ALKrearranged lung ADC. | Hoi-Hin Kwok Huiyu Li Jiashuang Yang Junyang Deng Nerissa Chui-Mei Lee Timmy Wing-Kuk Au Alva Ko-Yung Sit Michael Kuan-Yew Hsin Stephanie Kwai-Yee Ma LydiaWai-Ting Cheung Luc Girard Junya Fujimoto Ignacio Ivan Wistuba Boning Gao John Dorrance Minna David Chi-Leung Lam | 2023 | Cancer Communications2023，43，8： | 0 |
| 7 | Machine Learning Modeling of Protein-intrinsic Features Predicts Tractability of Targeted Protein Degradation显示文摘Targeted protein degradation(TPD)has rapidly emerged as a therapeutic modality to eliminate previously undruggable proteins by repurposing the cell’s endogenous protein degradation machinery.However,the susceptibility of proteins for targeting by TPD approaches,termed“degradability”,is largely unknown.Here,we developed a machine learning model,model-free analysis of protein degradability(MAPD),to predict degradability from features intrinsic to protein targets.MAPD shows accurate performance in predicting kinases that are degradable by TPD compounds[with an area under the precision–recall curve(AUPRC)of 0.759 and an area under the receiver operating characteristic curve(AUROC)of 0.775]and is likely generalizable to independent non-kinase proteins.We found five features with statistical significance to achieve optimal prediction,with ubiquitination potential being the most predictive.By structural modeling,we found that E2-accessible ubiquitination sites,but not lysine residues in general,are particularly associated with kinase degradability.Finally,we extended MAPD predictions to the entire proteome to find964 disease-causing proteins(including proteins encoded by 278 cancer genes)that may be tractable to TPD drug development. | Wubing Zhang Shourya S.Roy Burman Jiaye Chen Katherine A.Donovan Yang Cao Chelsea Shu Boning Zhang Zexian Zeng Shengqing Gu Yi Zhang Dian Li Eric S.Fischer Collin Tokheim X.Shirley Liu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，5： | 0 |
| 8 | Phase-Field Modeling of Hydrogen Diffusion and Trapping in Steels显示文摘Hydrogen embrittlement of steels is directly linked to hydrogen diffusion and trapping in the microstructure,which can hardly be precisely measured by modern experimental techniques.A phase-field model,in which a chemical potential well of hydrogen in the grain boundaries is introduced,is proposed to simulate hydrogen diffusion and trapping in the polycrystalline iron.It was interestingly found that grain boundaries,as connected trap sites,have a complex influence on the effective diffusivity of hydrogen,which are strongly linked to grain boundary diffusivity and binding energy. | Jun Zhang Jie Su Boning Zhang Yi Zong Zhigang Yang Chi Zhang Hao Chen | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，10： | 0 |
| 9 | Electronic and Spectroscopic Properties of La2@C112 Isomers显示文摘 | WANG Mingqian WANG Boning LI Weiqi ZHOU Xin YANG Li TIAN Weiquan | 2018 | Chemical Research in Chinese Universities2018，34，2： | 0 |
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