|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Determining antimicrobial resistance profiles and identifying novel mutations of Neisseria gonorrhoeae genomes obtained by multiplexed MinION sequencing显示文摘Gonorrhea is one of the most common sexually transmitted diseases worldwide. To cure infection and prevent transmission,timely and appropriate antimicrobial therapy is necessary. Unfortunately, Neisseria gonorrhoeae, the etiological agent of gonorrhea, has acquired nearly all known mechanisms of antimicrobial resistance(AMR), thereby compromising the efficacy of antimicrobial therapy. Treatment failure resulting from AMR has become a global public health concern. Whole-genome sequencing is an effective method to determine the AMR characteristics of N. gonorrhoeae. Compared with next-generation sequencing, the MinION sequencer(Oxford Nanopore Technologies(ONT)) has the advantages of long read length and portability. Based on a pilot study using MinION to sequence the genome of N. gonorrhoeae, we optimized the workflow of sequencing and data analysis in the current study. Here we sequenced nine isolates within one flow cell using a multiplexed sequencing strategy. After hybrid assembly with Illumina reads, nine integral circular chromosomes were obtained. By using the online tool Pathogenwatch and a BLAST-based workflow, we acquired complete AMR profiles related to seven classes of antibiotics. We also evaluated the performance of ONT-only assemblies. Most AMR determinants identified by ONT-only assemblies were the same as those identified by hybrid assemblies. Moreover, one of the nine assemblies indicated a potentially novel antimicrobial-related mutation located in mtrR which results in a frame-shift, premature stop codon, and truncated peptide.In addition, this is the first study using the MinION sequencer to obtain complete genome sequences of N. gonorrhoeae strains which are epidemic in China. This study shows that complete genome sequences and antimicrobial characteristics of N.gonorrhoeae can be obtained using the MinION sequencer in a simple and cost-effective manner, with hardly any knowledge of bioinformatics required. More importantly, this strategy provides us with a potential approach to discover new AMR determinants. | Chi Zhang Feng Wang Cansheng Zhu Leshan Xiu Yamei Li Li Li Bo Liu Yizhun Li Yaling Zeng Boyang Guo Junping Peng | 2020 | Science China(Life Sciences)2020，63，7： | 2 |
| 2 | Life cycle assessment of HFC-134a production by calcium carbide acetylene route in China显示文摘HFC-134a is a widely used environment-friendly refrigerant.At present,China is the largest producer of HFC-134a in the world.The production of HFC-134a in China mainly adopts the calcium carbide acetylene route.However,the production route has high resource and energy consumption and large waste emission,and few of the studies addressed on the environmental performance of its production process.This study quantified the environmental performance of HFC-134a production by calcium carbide route via carrying out a life cycle assessment(LCA)using the CML 2001 method.And uncertainty analysis by Monte-Carlo simulation was also carried out.The results showed that electricity had the most impact on the environment,followed by steam,hydrogen fluoride and chlorine,and the impact of direct CO_(2) emissions in calcium carbide production stage on the global warming effect also could not be ignored.Therefore,the clean energy(e.g.,wind,solar,biomass,and natural gas)was used to replace coal-based electricity and coal-fired steam in this study,showing considerable environmental benefits.At the same time,the use of advanced production technologies could also improve environmental benefits,and the environmental impact of the global warming category could be reduced by 4.1%via using CO_(2) capture and purification technology.The Chinese database of HFC-134a production established in this study provides convenience for the relevant study of scholars.For the production of HFC-134a,this study helps to better identify the specific environmental hotspots and proposes useful ways to improve the environmental benefits. | Suisui Zhang Jingying Li Yan Nie Luyao Qiang Boyang Bai Zhiwei Peng Xiaoxun Ma | 2022 | Chinese Journal of Chemical Engineering2022，35，2： | 1 |
| 3 | The tumor-enriched small molecule gambogic amide suppresses glioma by targeting WDR1-dependent cytoskeleton remodeling显示文摘Glioma is the most prevalent brain tumor,presenting with limited treatment options,while patients with malignant glioma and glioblastoma(GBM)have poor prognoses.The physical obstacle to drug delivery imposed by the blood‒brain barrier(BBB)and glioma stem cells(GSCs),which are widely recognized as crucial elements contributing to the unsatisfactory clinical outcomes.In this study,we found a small molecule,gambogic amide(GA-amide),exhibited the ability to effectively penetrate the blood-brain barrier(BBB)and displayed a notable enrichment within the tumor region.Moreover,GA-amide exhibited significant efficacy in inhibiting tumor growth across various in vivo glioma models,encompassing transgenic and primary patient-derived xenograft(PDX)models.We further performed a genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)knockout screen to determine the druggable target of GA-amide.By the combination of the cellular thermal shift assay(CETSA),the drug affinity responsive target stability(DARTS)approach,molecular docking simulation and surface plasmon resonance(SPR)analysis,WD repeat domain 1(WDR1)was identified as the direct binding target of GA-amide.Through direct interaction with WDR1,GA-amide promoted the formation of a complex involving WDR1,MYH9 and Cofilin,which accelerate the depolymerization of F-actin to inhibit the invasion of patient-derived glioma cells(PDCs)and induce PDC apoptosis via the mitochondrial apoptotic pathway.In conclusion,our study not only identified GA-amide as an effective and safe agent for treating glioma but also shed light on the underlying mechanisms of GA-amide from the perspective of cytoskeletal homeostasis. | Jiaorong Qu Bojun Qiu Yuxin Zhang Yan Hu Zhixing Wang Zhiang Guan Yiming Qin Tongtong Sui Fan Wu Boyang Li Wei Han Xiaozhong Peng | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 4 | Effect of Different Drying Processes on Active Ingredients of Rheum officinale Baill.显示文摘[Objectives]To determine the drying process of different functional rhubarbs.[Methods]The contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were determined by HPLC.The results were analyzed by two-way analysis of variance.[Results]Under different drying conditions,the contents of tannins,bianthrone,free anthraquinones and combined anthraquinones in medicinal rhubarb were significantly different.Taking the tannins content as the index,the optimum drying condition of the rhizome is 1 cm thick,dried at 60℃,the root is cut 1 cm thick,and dried at 30℃;the bianthrone content is used as an indicator,the rhizome is optimally dried.The condition is to cut 1 cm thick,dry at 60℃,the root is cut to 5 cm thick,and dried at 40℃;the free anthraquinones content is used as an index,and the optimum drying condition of the rhizome is 3 cm thick and dried at 50℃.The root is cut to a thickness of 3 cm and dried at 30℃.The combine anthraquinones content is used as an indicator.The optimum drying conditions for the rhizome are 5 cm thick,dried at 40℃,and the root is cut to 5 cm thick and dried at 70℃.[Conclusions]Different functional components of rhubarb have different directional processing methods.The drying process can reduce the drying temperature or increase the thickness of the slice,and the directional processing of the diarrhea-type rhubarb can be processed.The drying process can be carried out by increasing the drying temperature or reducing the thickness of the slice directed processing of heat-clearing and purging-fire rhubarb. | Yiming DING Tong SHANG Yue SHI Boyang PENG Ting ZHAO Yuan ZHANG Li LI Shengli WEI | 2020 | Medicinal Plant2020，11，6： | 0 |
| 5 | Off-road testing scenario design and library generation for intelligent vehicles显示文摘To realize the widespread application and continuous functional development of intelligent vehicles,test and evaluation of vehicle's functionality and Safety Performance in complex off-road scenarios are fundamental.Since traditional distance-based road tests cannot meet the evolving test requirements,a method to design the function-based off-road testing scenario library for intelligent vehicles(IV)is proposed in this paper.The testing scenario library is defined as a critical set of scenarios that can be used for IV tests.First,for the complex and diverse off-road scenarios,a hierarchical,structural model of the test scenario is built.Then,the critical test scenarios are selected adaptively according to the vehicle model to be tested.Next,those parameters representing the challenging test scenarios are selected.The selected parameters need to fit the natural distribution probability of scenarios.The critical test-scenario library is built combing these parameters with the structural model.Finally,the test scenarios that are most approximate to the natural driving scenario are determined with importance sampling theory.The test-scenario library built with this method can provide more critical test scenarios,and is widely applicable despite different vehicle models.Verified by simulation in the off-road interaction scenarios,test would be accelerated significantly with this method,about 800 times faster than testing in the natural road environment. | Yuchun Wang Jianwei Gong Boyang Wang Peng Jia Tansyou Kyo | 2022 | Green Energy and Intelligent Transportation2022，1，3： | 0 |
| 6 | The effect of hydrothermal pretreatment on the structure and fast pyrolysis behaviors of Sheng Li lignite显示文摘The structure and composition of coal determine its fast pyrolysis characteristics,and the study of the relationship between them can play an important role in the efficient and clean utilization of coal.So,in this work,hydrothermal pretreatment was used to artificially change the structure and composition of Sheng Li(SL)lignite,which was used to investigate the influence of structural changes on pyrolysis.The physicochemical structure and composition of samples were characterized by X-ray diffraction,specific surface area and porosity analyzer,solid-state 13C nuclear magnetic resonance,Fourier transform infrared spectroscopy,and elemental analyzer.Pyrolysis experiments were carried out in a powderparticle fluidized bed reactor,and the distribution and composition of the pyrolysis products were analyzed.The gasification activity of char was investigated by thermogravimetric analysis with a CO_(2) atmosphere.The results show that hydrothermal pretreatment(HTP)can destroy the cross-linking structure of SL lignite,and affect its aromaticity,pore structure,functional group,and carbon structure to change the distribution and composition of pyrolysis products of SL lignite,especially the composition of tar.Finally,the structure–activity relationship between the structure,composition,and pyrolysis characteristics of coal was comprehensively studied. | Boyang Bai Luyao Qiang Suisui Zhang Zhiwei Peng Hang Mu Xiaoxun Ma | 2021 | Chinese Journal of Chemical Engineering2021，34，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员