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| 1 | Bak在细胞凋亡期间对线粒体形态学的影响显示文摘目的:探讨Bak基因在细胞凋亡线粒体信号传导中的作用以及与Bax基因之间的相互关系。方法:应用Bax/Bak双重基因敲除的小鼠胚胎成纤维细胞(MEF)和Hela细胞进行基因重构,细胞按不同基因型(野生型、Bax敲除和Bak敲除、双重基因敲除)分组,并通过共转染方式导入目的基因。细胞凋亡的处理条件:细胞在无葡萄糖缓冲液的培养基中,在含10mmol.L-1叠氮钠或1μmol.L-1十字孢碱(STS)或20μmol.L-1顺铂条件下培养,并诱导细胞凋亡。应用共聚焦显微镜和免疫荧光及免疫印记技术分析细胞凋亡、线粒体碎裂和细胞色素C释放情况,综合评价Bak基因在细胞凋亡和线粒体碎裂中的作用。结果:Hela细胞内线粒体碎裂和细胞色素C释放百分率分别与细胞凋亡有关联(P<0.01);MEF细胞在Bak基因缺失(Bak-/-)时,应用化学诱导剂导致的细胞凋亡率(17.9%)明显低于野生型MEF细胞(71.3%)(P<0.01);且Bax和Bak基因两者协同作用可以增强细胞凋亡发生率;当pEGFP-Bak重新导入Bax-/-/Bak-/-MEF细胞,线粒体破碎百分率从43.7%增加到76.4%,pEGFP-Bak组细胞凋亡率明显高于pEGFP-Bax组(P<0.05);应用不同的化学诱导剂均可以得到同样的结果;且Bcl-2不能够明显阻止Bak诱导的细胞凋亡。结论:Bak基因明确诱导线粒体碎裂和细胞色素C释放,导致细胞凋亡,其诱导细胞凋亡的效果明显大于Bax基因,并且其作用独立于Bax基因功能之外。 | 冯乐平 乔伟 Craig Brooks Zeng Dong | 2008 | 吉林大学学报(医学版)2008，34，5： | 5 |
| 2 | The amazing potential of fungi:50 ways we can exploit fungi industrially显示文摘Fungi are an understudied,biotechnologically valuable group of organisms.Due to the immense range of habitats that fungi inhabit,and the consequent need to compete against a diverse array of other fungi,bacteria,and animals,fungi have developed numerous survival mechanisms.The unique attributes of fungi thus herald great promise for their application in biotechnology and industry.Moreover,fungi can be grown with relative ease,making production at scale viable.The search for fungal biodiversity,and the construction of a living fungi collection,both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products.This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology.We provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable researchers.We also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products.Fungi have provided the world with penicillin,lovastatin,and other globally significant medicines,and they remain an untapped resource with enormous industrial potential. | Kevin D.Hyde Jianchu Xu Sylvie Rapior Rajesh Jeewon Saisamorn Lumyong Allen Grace T.Niego Pranami D.Abeywickrama Janith V.S.Aluthmuhandiram Rashika S.Brahamanage Siraprapa Brooks Amornrat Chaiyasen K.W.Thilini Chethana Putarak Chomnunti Clara Chepkirui Boontiya Chuankid Nimali I.de Silva Mingkwan Doilom Craig Faulds Eleni Gentekaki Venkat Gopalan Pattana Kakumyan Dulanjalee Harishchandra Hridya Hemachandran Sinang Hongsanan Anuruddha Karunarathna Samantha C.Karunarathna Sehroon Khan Jaturong Kumla Ruvishika S.Jayawardena Jian-Kui Liu Ningguo Liu Thatsanee Luangharn Allan Patrick G.Macabeo Diana S.Marasinghe Dan Meeks Peter E.Mortimer Peter Mueller Sadia Nadir Karaba N.Nataraja Sureeporn Nontachaiyapoom Meghan O’Brien Watsana Penkhrue Chayanard Phukhamsakda Uma Shaanker Ramanan Achala R.Rathnayaka Resurreccion B.Sadaba Birthe Sandargo Binu C.Samarakoon Danushka S.Tennakoon Ramamoorthy Siva Wasan Sriprom T.S.Suryanarayanan Kanaporn Sujarit Nakarin Suwannarach Thitipone Suwunwong Benjarong Thongbai Naritsada Thongklang Deping Wei S.Nuwanthika Wijesinghe Jake Winiski Jiye Yan Erandi Yasanthika Marc Stadler | 2019 | Fungal Diversity2019，，4： | 3 |
| 3 | Frag- mented mitochondria are sensitized to Bax insertion and acti- vation during apoptosis 显示文摘 | Craig Brooks Sung - Gyu Cho Cong - Yi Wang | 2011 | Am J Physiol Cell Physiol2011，300，2： | 1 |
| 4 | Consensus-Derived Practice Standards Plan for Complicated Kaposiform Hemangioendothelioma显示文摘 | Beth A. Drolet Cameron C. Trenor Leonardo R. Brand?o Yvonne E. Chiu Robert H. Chun Roshni Dasgupta Maria C. Garzon Adrienne M. Hammill Craig M. Johnson Brook Tlougan Francine Blei Michèle David Ravindhra Elluru Ilona J. Frieden Sheila F. Friedlander Ionel | 2013 | The Journal of Pediatrics2013，，1： | 1 |
| 5 | Tailoring the structural integrity process to meet the challenges of aging aircraft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，，： | 1 |
| 6 | Tailoring the structural integrity process to meet the challenges of aging aircraft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，21，： | 1 |
| 7 | Tailoring the structural integrity pro- cess to meet the challenges of aging aircraft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，21，： | 1 |
| 8 | How negative affectivity moderates the relationship between shocks, embeddedness and worker behaviors显示文摘 | Brooks C. Holtom James P. Burton Craig D. Crossley | 2012 | Journal of Vocational Behavior2012，，2： | 1 |
| 9 | Ileocecal endometriosis presenting with abdominal pain and gastrointestinal bleeding显示文摘 | Craig A. Aronchick MD Dr. Frank P. Brooks MD William L. Dyson MD Richard Baron MD John J. Thompson MD | 1983 | Digestive Diseases and Sciences1983，，6： | 1 |
| 10 | Tailoring the structural integrity process to meet the challenges of aging aircraft 显示文摘 | DAVID L SIMPSON CRAIG L BROOKS | 1999 | International Journal of Fatigue1999，21，： | 1 |
| 11 | Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins 显示文摘 | Brooks Craig Wei Qing-qing Feng Le-ping | 2007 | Proc Natil Acad Sci2007，104，11： | 1 |
| 12 | Somatotopic organisation of the human insula to painful heat studied with high resolution functional imaging显示文摘 | J.C.W. Brooks L. Zambreanu A. Godinez A.D. (Bud) Craig I. Tracey | 2005 | Neuroimage2005，，1： | 1 |
| 13 | Tailoring the structural integrity process to meet the challenges of aging aircraft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，21，： | 1 |
| 14 | Tailoring the structural integrity process to meet the challenges of aging aircraft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，21，： | 1 |
| 15 | Tailoring the structural integrity process to meet the challenges of aging aireroft显示文摘 | David L Simpson Craig L Brooks | 1999 | International Journal of Fatigue1999，21，： | 1 |
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