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| 1 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 2 | The possible subduction of continental material to depths greater than 200 km 显示文摘 | Ye Kai Cong Bailin Ye Danian | 2000 | Nature2000，407，： | 1 |
| 3 | Effects of hydrogen sulfide on hypoxic pulmonary vascular structural remodeling显示文摘 | Hongfang J Cong Bailin Zhao Bin | 2006 | L ifeSci2006，78，12： | 1 |
| 4 | Effects of hydrogen sulfide on hypoxic pulmonary vascular structural remodeling 显示文摘 | HONGFANG J CONG BAILIN ZHAO BIN | 2006 | Life Sci2006，78，12： | 1 |
| 5 | Effects of hydrogen sulfide on hypoxic pulmonary vascular structural remodeling显示文摘 | HONG FANG J CONG BAILIN ZHAO BIN | | 0，，： | 1 |
| 6 | Effects of hydrogensulfide on hypoxic pulmonary vascular structural remodeling显示文摘 | Hongfang J Cong Bailin Zhao Bin | | 0，，： | 1 |
| 7 | Efeets of hydrogen sul- fide on hypoxie pulmonary vascular structural remodeling显示文摘 | Hongfang J Cong Bailin Zhao Bin | 2006 | Life Sei2006，78，12： | 1 |
| 8 | Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus)显示文摘In order to provide pertinent evidence of ion channel with immune response in the fish,whole cell patch-clamp technique was employed for potassium ion channel study in turbot(Scophthalmus maximus) . Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples,and electrophysiological characters of the channel were analyzed. In the recorded cells,activated voltage of the channels was-42.5 ± 3.7 mV and the average peak current was 313.12 ± 28.2 pA. The channel was identified as voltage dependent,the current was outward and it could be inhibited by 10 mmol/dm 3 TEA or 5 mmol/dm 3 4-AP,a specific potassium channel inhibitor,identifying the existence of potassium channel in peripheral lymphocytes of the turbot. | CHAI Yingmei HUANG Xiaohang CONG Bailin LIU Chenlin LIU Shenghao | 2010 | Acta Oceanologica Sinica2010，29，1： | 0 |
| 9 | Phylogenetic analysis and in vitro culture of mosses fromthe Antarctic Fildes Peninsula显示文摘Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species.In the present study,the small subunit ribosomal RNA(SSU rDNA)and internal transcribed spacers of the nuclear ribosomal DNA(ITS rDNA)were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica.Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses.In addition,in our phylogenetic analysis,one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species.Moreover,the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated.The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation. | LIU Shenghao ZHANG Zhaohui WANG Nengfei CONG Bailin ZHANG Pengying LIN Xuezheng HUANG Xiaohang | 2014 | Advances in Polar Science2014，25，2： | 0 |
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