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| 1 | NMR-based metabolomic analysis for the effects of creatine supplementation on mouse myoblast cell line C2C12显示文摘肌酸(Cr ) 补充最近在运动,和更多由于它的普遍功效从研究人员引起了许多注意,在治疗学的领域里。然而,内在的分子的机制留下逃犯。这里,我们执行了原子磁性的基于回声的 metabolomic 分析从为 24 h (对待 Cr 的组) 暴露于 2 公里 Cr 的老鼠 myoblast 房间线 C2C12 探讨水的摘录的新陈代谢的侧面。结果证明 Cr 补充便于 C2C12 myoblasts 的增长。模式识别和层次簇分析证明新陈代谢的侧面对待 Cr 并且控制组是显然不同的。我们识别了为在二个组之间的新陈代谢的侧面的辨别显著地负责的 13 典型代谢物,通过到潜伏的结构判别式分析和独立样品 t 测试的直角的设计。我们进一步由进行操作典型曲线分析的 univariate 接收装置验证了这些代谢物的辨别表演。与控制组相比,展出的对待 Cr 的组增加了 Cr, phosphocreatine (PCr ) ,谷胱甘肽(GSH ) ,和葡萄糖的层次,但是减少白氨酸,缬氨酸,异白氨酸,本氨基丙酸,蛋氨酸,胆碱, O-phosphocholine, sn-glycero-3-phosphocholine,和甘油的层次。我们的结果表明了那 Cr 补充 upregulated PCr 和葡萄糖,支持了 trichloroacetic 酸周期 anaplerotic 流动和调停 GSH 的抗氧化剂能力,并且稳定了通过压制 glycerophospholipid 新陈代谢的类脂化合物膜。我们的工作提供新线索给在肌肉房间位于 Cr 的多种的效果下面的分子的机制。 | Wenqi Xu Donghai Lin Caihua Huang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 2 |
| 2 | Rho 1 participates in parasitoid wasp eggs maturation and host cellular immunity inhibition显示文摘Endoparasitoid wasps introduce venom into their host insects during the egglaying stage.Venom proteins play various roles in the host physiology,development,immunity,and behavior manipulation and regulation.In this study,we identified a venom protein,MmRhol,a small guanine nucleotide-binding protein derived from ovary in the endoparasitoid wasp Microplitis mediator and found that knockdown of its expression by RNA interference caused down-regulation of vitellogenin and juvenile hormone,egg production,and cocoons formation in the female wasps.We demonstrated that MmRho1 entered the cotton bollworm's(host)hemocytes and suppressed cellular immune responses after parasitism using immunofluorescence staining.Furthermore,wasp MmRhol interacted with the cotton bollworm's actin cytoskeleton rearrangement regulator diaphanous by yeast 2-hybrid and glutathione s-transferase pull-down.In conclusion,this study indicates that MmRho1 plays dual roles in wasp development and the suppression of the host insect cellular immune responses. | Ruijuan Wang Zhe Lin Lizhen Zhou Caihua Chen Xianhao Yu Junje Zhang Zhen Zou Zhiqiang Lu | 2023 | Insect Science2023，30，3： | 1 |
| 3 | Equivalent Impedance Parameter Calculation of Three-phase Symmetrical Loads for Harmonic Source Location显示文摘The equivalent impedance parameters of loads have been widely used to identify and locate the harmonic sources.However,the existing calculation methods suffer from outliers caused by the zero-crossing of the denominator.These outliers can result in inaccuracy and unreliability of harmonic source location.To address this issue,this paper proposes an innovative method of equivalent impedance parameter calculation of three-phase symmetrical loads that avoid outliers.The correctness and effectiveness of the proposed method are verified by simulations on Simulink using actual monitoring data.The results show that the proposed method is not only simple and easy to implement but also highly accurate. | Yi Zhang Bijie Liu Caihua Lin Zhenguo Shao Yuncong Xu | 2024 | Journal of Modern Power Systems and Clean Energy2024，12，1： | 0 |
| 4 | Metabolic signatures and potential biomarkers for the diagnosis and treaeatment of colon cancer cachexia显示文摘Cancer cachexia(CAC)is a debilitating condition that often arises from noncachexia cancer(NCAC),with distinct metabolic characteristics and medical treatments.However,the metabolic changes and underlying molecular mechanisms during cachexia progression remain poorly understood.Understanding the progression of CAC is crucial for developing diagnostic approaches to distinguish between CAC and NCAC stages,facilitating appropriate treatment for cancer patients.In this study,we establish a mouse model of colon CAC and categorize the mice into three groups:CAC,NCAC and normal control(NOR).By performing nuclear magnetic resonance(NMR)-based metabolomic profiling on mouse sera,we elucidate the metabolic properties of these groups.Our findings unveil significant differences in the metabolic profiles among the CAC,NCAC and NOR groups,highlighting significant impairments in energy metabolism and amino acid metabolism during cachexia progression.Additionally,we observe the elevated serum levels of lysine and acetate during the transition from the NCAC to CAC stages.Using multivariate ROC analysis,we identify lysine and acetate as potential biomarkers for distinguishing between CAC and NCAC stages.These biomarkers hold promise for the diagnosis of CAC from noncachexia cancer.Our study provides novel insights into the metabolic mechanisms underlying cachexia progression and offers valuable avenues for the diagnosis and treatment of CAC in clinical settings. | Xu Qiu Ruohan Lu Qiqing He Shu Chen Caihua Huang Donghai Lin | 2023 | Acta Biochimica et Biophysica Sinica2023，55，12： | 0 |
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