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| 1 | Two CYP82D Enzymes Function as Flavone Hydroxylases in the Biosynthesis of Root-Specific 4'-Deoxyflavones in Scutellaria baicalensis显示文摘Baicalein, wogonin,和他们的 glycosides 是在药用的植物黄芩 baicalensis Georgi 发现的主要 bioactive 混合物。这些黄酮能在许多癌症房间线导致 apoptosis,但是没在正常房间上有效果。而且,他们为人的健康有许多另外的好处,例如抗氧化剂,抗病毒、肝保护的性质。这里,我们报导二 CYP450 酶, SbCYP82D1.1 和 SbCYP82D2 的隔离和描述,分别地,它在 S. 作为黄酮 6-hydroxylase (F6H ) 和黄酮 8-hydroxylase (F8H ) 工作 ? baicalensis。SbCYP82D1.1 为象 chrysin 和 apigenin 那样的黄酮有宽广底层特性并且在根和 S. 的天线部分为 baicalein 和 scutellarein 的生合成负责 ? baicalensis 分别地。当 SbCYP82D1.1 的表示被击倒时,当时, baicalin 和 baicalein 层次显著地被减少 chrysin glycosides 在毛乎乎的根积累。SbCYP82D2 是有高底层特性的 F8H,接受仅仅象它生产 norwogonin 的底层的 chrysin,尽管次要的 6-hydroxylation 活动能也被检测。种系发生的分析建议 SbCYP82D2 可能经由 neofunctionalization 跟随的基因复制从 SbCYP82D1.1 演变,祖先的 F6H 活动由此部分在导出的 SbCYP82D2 被保留。 | Qing Zhao Meng-Ying Cui Olesya Levsh Dongfeng Yang Jie Liu Jie Li Lionel Hill Lei Yang Yonghong Hu Jing-Ke Weng Xiao-Ya Chen Cathie Martin | 2018 | Molecular Plant2018，11，1： | 30 |
| 2 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 3 | Metformin does not improve survival in patients with hepatocellular carcinoma显示文摘AIM:To assess whether metformin,which has a chemopreventive effect in chronic liver disease,has any chemotherapeutic effect in hepatocellular carcinoma.METHODS:This was a retrospective study of 701 patients with newly diagnosed hepatocellular carcinoma(HCC)seen between January 2005 and June 2011 at Mayo Clinic,Rochester,Minnesota.This patient cohort was a part of the global HCC BRIDGE study,which is a large longitudinal study of HCC determining the realworld experience of HCC characteristics,management and patient outcomes.We defined significant metformin exposure as continuation of this agent at least 90d beyond diagnosis of HCC,and compared survival of diabetic patients on metformin to diabetic patients not on metformin and non-diabetics.RESULTS:Our cohort was 72.9%male,with a mean±SD age of 62.6±12.3 years.The most common etiologies of liver disease were hepatitis C(34%),alcoholic liver disease(29%),fatty liver disease(15%)and hepatitis B(9%).By univariate analysis,using diabetics not on metformin as the reference group,diabetic patients with HCC on metformin had no survival advantage,with a HR(95%CI)of 1.0(0.8-1.3).Non-diabetic HCC patients also did not appear to have a survival advantage as compared to diabetic HCC patients not on metformin,as demonstrated by a HR(95%CI)of1.1(0.7-1.7).Diabetics on metformin beyond 90 d after HCC diagnosis had a longer median survival at 34.2 mo,as compared to 25.5 mo among diabetic patients who were not on metformin or had discontinued metformin within 90 d after HCC diagnosis.This finding was likely due to potential survival bias among those who lived long enough to receive metformin.CONCLUSION:Although the literature suggests a chemotherapeutic effect in other malignancies,our study demonstrates no survival benefit to the use of metformin in diabetic patients with HCC. | Mamatha Bhat Roongruedee Chaiteerakij William S Harmsen Cathy D Schleck Ju Dong Yang Nasra H Giama Terry M Therneau Gregory J Gores Lewis R Roberts | 2014 | World Journal of Gastroenterology2014，20，42： | 11 |
| 4 | Next-Generation Plant Metabolic Engineering, Inspired by an Ancient Chinese Irrigation System显示文摘专业化第二等的代谢物服务不仅保护植物免于不能生活、关於生命的挑战,而且也有由人广泛地使用与疾病作斗争。由于 ? 为健康的药用的植物的大重要性,我们需要发现改进 ?的生产的新、持续的方法专业化代谢物。除了直接抽取,在植物的新陈代谢的工程的最近的进步提供一种其他的供应选择。我们主张为在植物生产第二等的代谢物的新陈代谢的工程可以比微生物引起的生产平台有明显的优点,并且因此建议新途径种新陈代谢的工程,它被一个古老的中国灌溉系统启发。新陈代谢的工程策略在三个层次工作:介绍 biosynthetic 基因,用抄写因素,并且改进新陈代谢的流动包括增加先锋,精力,和减少的电源的供应。另外,在生物工学的最近的进步显著地作出贡献到更好设计,例如特定的倡导者的使用 ? 并且竞争分支小径的删除。我们建议那家下一代的工厂新陈代谢的设计将改进当前的工程策略,为生产在工厂的珍贵代谢物的目的 ? 在工业规模上。 | Rao Fu Cathie Martin Yang Zhang | 2018 | Molecular Plant2018，11，1： | 7 |
| 5 | The genomes of medicinal skullcaps reveal the polyphyletic origins of clerodane diterpene biosynthesis in the family Lamiaceae显示文摘The presence of anticancer clerodane diterpenoids is a chemotaxonomic marker for the traditional Chinese medicinal plant Scutellaria barbata,although the molecular mechanisms behind clerodane biosynthesis are unknown.Here,we report a high-quality assembly of the 414.98 Mb genome of S.barbata into 13 pseudochromosomes.Using phylogenomic and biochemical data,we mapped the plastidial metabolism of kaurene(gibberellins),abietane,and clerodane diterpenes in three species of the family Lamiaceae(Scutellaria barbata,Scutellaria baicalensis,and Salvia splendens),facilitating the identification of genes involved in the biosynthesis of the clerodanes,kolavenol,and isokolavenol.We show that clerodane biosynthesis evolved through recruitment and neofunctionalization of genes from gibberellin and abietane metabolism.Despite the assumed monophyletic origin of clerodane biosynthesis,which is widespread in species of the Lamiaceae,our data show distinct evolutionary lineages and suggest polyphyletic origins of clerodane biosynthesis in the family Lamiaceae.Our study not only provides significant insights into the evolution of clerodane biosynthetic pathways in the mint family,Lamiaceae,but also will facilitate the production of anticancer clerodanes through future metabolic engineering efforts. | Haixiu Li Song Wu Ruoxi Lin Yiren Xiao Ana Luisa Malaco Morotti Ya Wang Meytal Galilee Haowen Qin Tao Huang Yong Zhao Xun Zhou Jun Yang Qing Zhao Angelos K.Kanellis Cathie Martin Evangelos C.Tatsis | 2023 | Molecular Plant2023，16，3： | 2 |
| 6 | SbMYB3 transcription factor promotes root-specific f lavone biosynthesis in Scutellaria baicalensis显示文摘Scutellaria baicalensis Georgi produces abundant root-specific f lavones(RSFs),which provide various benefits to human health.We have elucidated the complete biosynthetic pathways of baicalein and wogonin.However,the transcriptional regulation of f lavone biosynthesis in S.baicalensis remains unclear.We show that the SbMYB3 transcription factor functions as a transcriptional activator involved in the biosynthesis of RSFs in S.baicalensis.Yeast one-hybrid and transcriptional activation assays showed that SbMYB3 binds to the promoter of flavone synthase II-2(SbFNSII-2)and enhances its transcription.In S.baicalensis hairy roots,RNAi of SbMYB3 reduced the accumulation of baicalin and wogonoside,and SbMYB3 knockout decreased the biosynthesis of baicalein,baicalin,wogonin,and wogonoside,whereas SbMYB3 overexpression enhanced the contents of baicalein,baicalin,wogonin,and wogonoside.Transcript profiling by qRT–PCR demonstrated that SbMYB3 activates SbFNSII-2 expression directly,thus leading to more abundant accumulation of RSFs.This study provides a potential target for metabolic engineering of RSFs. | Yumin Fang Jie Liu Minmin Zheng Sanming Zhu Tianlin Pei Mengying Cui Lijing Chang Hanwen Xiao Jun Yang Cathie Martin Qing Zhao | 2023 | Horticulture Research2023，10，2： | 2 |
| 7 | AlGaN-GaN HEMTs on SiC With CW Power Performance of >4 W/ram and 23% PAE at 35 GHz显示文摘 | Cathy Lee Paul Saunier Jinwei Yang | 2003 | IEEE Electron Device Letters2003，24，10： | 1 |
| 8 | The asymmetric reactions of mean and volatility of stock returns to domestic and international information based on a four- regime double-threshold GARCH model显示文摘 | Cathy W.S. Chen Ming Jing Yang Richard Gerlach H.Jim Lo | | 0，，： | 1 |
| 9 | Succinate Dehydrogenase Kidney Cancer: An Aggressive Example of the Warburg Effect in Cancer显示文摘 | Christopher J. Ricketts Brian Shuch Cathy D. Vocke Adam R. Metwalli Gennady Bratslavsky Lindsay Middelton Youfeng Yang Ming-Hui Wei Stephen E. Pautler James Peterson Catherine A. Stolle Berton Zbar Maria J. Merino Laura S. Schmidt Peter A. Pinto Ramaprasa | 2012 | The Journal of Urology2012，，： | 1 |
| 10 | Effect of succinimide ring modificationon N-(3,5-- dichlorophenyl) succinimide-induced nephrotoxicity in Sprague-Dawley and Fischer 344 rats显示文摘 | David J Yang Cathy D Rchmond Vonda J Teets | 1985 | Toxicology1985，37，： | 1 |
| 11 | Divergent trajectories of lean vs obese non-alcoholic steatohepatitis patients from listing to post-transplant:A retrospective cohort study显示文摘BACKGROUND Non-alcoholic steatohepatitis(NASH)cirrhosis is the second most common indication for liver transplantation(LT).The role of body mass index(BMI)on outcomes of NASH cirrhosis has been conflicting.AIM To compare the longitudinal trajectories of patients with lean vs obese NASH cirrhosis,from listing up to post-transplant,having adjusted their BMI for ascites.METHODS We retrospectively reviewed all adult NASH patients listed for LT in our program from 2012 to 2019.Fine-Gray Competing Risk analyses and Cox Proportional-Hazard Models were performed to examine the cumulative incidence of transplant and survival outcomes respectively.RESULTS Out of 265 NASH cirrhosis listed patients,176 were included.Median age was 61.0 years;46%were females.111 patients underwent LT.Obese robust patients had better waitlist survival[hazard ratio(HR):0.12;95%CI:0.05–0.29,P<0.0001]with higher instantaneous rate of transplant(HR:5.71;95%CI:1.26–25.9,P=0.02).Lean NASH patients had a substantially higher risk of graft loss within 90 d post-LT(1.2%vs 13.8%,P=0.032)and death post-LT(2.4%vs 17.2%,P=0.029).1-3-and 5-year graft survival was poor for lean NASH(78.6%,77.3%and 41.7%vs 98.6%,96%and 85%respectively).Overall patient survival post-LT was significantly worse in lean NASH(HR:0.17;95%CI:0.03–0.86,P=0.0142)with 83%lower instantaneous rate of death in obese group.CONCLUSION Although lean NASH is considered to be more benign than obese NASH,our study suggests a paradoxical correlation of lean NASH with waitlist outcomes,and graft and patient survival post-LT. | Fakhar Ali Qazi-Arisar Raj Uchila Catherine Chen Cathy Yang Shi-Yi Chen Ravikiran Sindhuvalada Karnam Amirhossein Azhie Wei Xu Zita Galvin Nazia Selzner Leslie Lilly Mamatha Bhat | 2022 | World Journal of Gastroenterology2022，28，26： | 1 |
| 12 | The UOK 257 cell line: a novel model for studies of the human Birt–Hogg–Dubé gene pathway显示文摘 | Youfeng Yang Hesed M. Padilla-Nash Manish A. Vira Mones S. Abu-Asab Daniel Val Robert Worrell Maria Tsokos Maria J. Merino Christian P. Pavlovich Thomas Ried W. Marston Linehan Cathy D. Vocke | 2008 | Cancer Genetics and Cytogenetics2008，，2： | 1 |
| 13 | Dow Corning Sign Syndicated Loan Agreement to Finance Zhanqiiaqanq Plant | Cathy Yang | 2009 | China Chemical Reporter2009，，13： | 0 |
| 14 | 2006 年秋季高级口译点评之翻译篇显示文摘 | Eric Hu Cathy Zhu Denis Yang | 2007 | 新东方英语(中英文版)2007，0，Z1： | 0 |
| 15 | Diterpenoids from Scutellaria barbata induce tumour-selective cytotoxicity by taking the brakes off apoptosis显示文摘Medicinal plants are an excellent source of structurally diverse,bio-active compounds with potential in the fight against cancer.One of the most promising is Scutellaria barbata,prescribed traditionally for the treatment of cancers.Scutebarbatine A is the major diterpenoid,produced in specialized large,peltate trichomes on leaves of S.barbata.It induces dose-dependent apoptosis,specifically in cancer cells.The major class of proteins down-regulated are pro-survival proteins,the Inhibitors of Apoptosis(IAPs),and IAP regulating proteins.We propose that scutebarbatine A works by releasing the molecular brakes(the IAPs)on apoptosis in cell death-evading cancer cells.Comparison between the cytotoxicity of methanolic extracts of S.barbata leaves and decoctions(Ban Zhi Lian)prepared traditionally,showed substantially different chemical compositions and differential induction of apoptosis.Analyses suggest polyvalency between the constituents in both extracts,and ways to produce enhanced chemopreventive preparations for the treatment of cancer. | Matthew L.Tomlinson Man Zhao Elaine J.Barclay Jie Li Haixiu Li Juri Felix Lionel Hill Gerhard Saalbach Martin Rejzek Dongfeng Yang Qing Zhao Paul Kroon Wei Wang Yongping Bao Melanie-Jayne R.Howes Evangelos C.Tatsis Cathie Martin | 2022 | Medicinal Plant Biology2022，1，1： | 0 |
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