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| 1 | On-chip multiplexed single-cell patterning and controllable intracellular delivery显示文摘Conventional electroporation approaches show limitations in the delivery of macromolecules in vitro and in vivo.These limitations include low efficiency,noticeable cell damage and nonuniform delivery of cells.Here,we present a simple 3D electroporation platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules.A pyramid pit micropore array chip was fabricated based on a silicon wet-etching method.A controllable vacuum system was adopted to trap a single cell on each micropore.Using this chip,safe single-cell electroporation was performed at low voltage.Cargoes of various sizes ranging from oligonucleotides(molecular beacons,22 bp)to plasmid DNA(CRISPR-Cas9 expression vectors,>9 kb)were delivered into targeted cells with a significantly higher transfection efficiency than that of multiple benchmark methods(e.g.,commercial electroporation devices and Lipofectamine).The delivered dose of the chemotherapeutic drug could be controlled by adjusting the applied voltage.By using CRISPR-Cas9 transfection with this system,the p62 gene and CXCR7 gene were knocked out in tumor cells,which effectively inhibited their cellular activity.Overall,this vacuum-assisted micropore array platform provides a simple,efficient,high-throughput intracellular delivery method that may facilitate on-chip cell manipulation,intracellular investigation and cancer therapy. | Zaizai Dong Yanli Jiao Bingteng Xie Yongcun Hao Pan Wang Yuanyuan Liu Junfeng Shi Chandani Chitrakar Stephen Black Yu-Chieh Wang LJames Lee Mo Li Yubo Fan Lingqian Chang | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
| 2 | Serological markers in Arabs with lupus nephritis显示文摘 | Al Attia HM Al Ahmed YH Chandani AU | | 0，，03： | 1 |
| 3 | Direct silicon-silicon bonding by electromagnetic induction heating显示文摘 | Keith Thompson Yogesh B Gian Chandani | 2002 | JMEMS2002，11，： | 1 |
| 4 | Antioxidant properties of green and black tea,and their potential ability to retard the progression of eye lens cataract显示文摘 | Thiagarajan G Chandani S Sundari CS | 2001 | Exp Eye Res2001，73，3： | 1 |
| 5 | Interactions be-tween plant growth promoting fungi and arbuscular mycorrhizal fungus Glomus mosseae and induction of systemic resistance to anthracnose disease in cucumber 显示文摘 | Chandanie WA Kubota M Hyakumachi M | 2006 | Plant Soil2006，286，12： | 1 |
| 6 | Interactions between plant growth promoting fungi and arbuscular mycorrhizal fungus Glomus mosseae and induction of systemic resistance to anthracnose disease in cucumber显示文摘 | Chandanie W A Kubota M Hyakumachi M | 2006 | Plant and Soil2006，286，12： | 1 |
| 7 | Interactions between plant growth promoting fungi and arbuscular mycorrhizal fungus Glomus mosseae and induction of systemic resistance to anthracnose disease in cucumber显示文摘 | Chandanie W A Kubota M Hyakumachi M | 2006 | Plant Soil2006，286，12： | 1 |
| 8 | Molecular and cellular assessment of ginkgo biloba extract as a possible ophthalmic drug显示文摘 | Thiagarajan G Chandani S Harinarayana Rao S | | 0，，： | 1 |
| 9 | Serological markers in Ar abs with lupus nephritis显示文摘 | Attia HM Ahmed YH Chandani AU | 1998 | Lupus1998，7，3： | 1 |
| 10 | Fiber-optic temperature sensor using evanescent fields in D fibers显示文摘 | Chandani S M Jaeger N A | 2005 | IEEE Photonics Technology Letters2005，17，12： | 1 |
| 11 | Interactions between plant growth promoting fungi and arbnscular mycorrhizal fungus Glomus mosseae and induction of systemic resistance to anthracnose disease in cucumber显示文摘 | Chandanie W A Kubota M Hyakumachi M | 2006 | Plant Soii2006，，286： | 1 |
| 12 | Developing a strategy for activity-based detection of enzymes in a protein microarray显示文摘 | Chen GY Uttam chandani M Zhu Q | 2003 | Chem Bio Chem2003，4，4： | 1 |
| 13 | Molecular and cellular assessment of biloba extract as a possible ophthalmic drug显示文摘 | Thiagarajan G Chandani S Harinaraya RS | 2002 | Exp Eye Res2002，75，： | 1 |
| 14 | Serological markers in Arabs with lupus nephritis显示文摘 | Al Attia HM Al Ahmed YH Chandani AU | 1998 | Lupus1998，7，： | 1 |
| 15 | Interactions between plant growth promoting fungi and arbuscular mycorrhizal fungus Glomus mosseae and induction of systemic resistance to anthracnose disease in cucumber显示文摘 | Chandanie W A Kubota M Hyakumachi M | 2006 | Plant Soil2006，286，12： | 1 |
| 16 | Molecular and Cellular Assessment of Ginkgo Biloba Extract as a Possible Ophthalmic Drug显示文摘 | THIAGARAJAN G CHANDANI S RAO SH | 2002 | Experimental Eye Research2002，75，4： | 1 |
| 17 | Molecular and Cellular Assessment of Ginkgo Biloba Extract as a Possible Ophthalmic Drug显示文摘 | Geetha Thiagarajan Sushil Chandani Sithamraju Harinarayana Rao Ayelet M. Samuni Krish Chandrasekaran Dorairajan Balasubramanian | 2002 | Experimental Eye Research2002，，4： | 1 |
| 18 | Molecular and Cellular Assessment of Ginkgo Biloba Extract as a Possible Ophthalmic Drug显示文摘 | Thiagarajan G Chandani S Rao SH | 2002 | Experimental Eye Research2002，75，4： | 1 |
| 19 | Fiber optic temperature sensor using evanescent fields in D fiber 显示文摘 | S M Chandani N A F Jaeger | 2007 | IEEE Photonics Technology Letters2007，17，12： | 1 |
| 20 | Molecular and Cellular Assessment of Ginkgo Biloba Extract as a Possible Ophthalmic Drug显示文摘 | THIAGARAJAN G CHANDANI S RAOSH | 2002 | Experimental Eye Research2002，75，4： | 1 |
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