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| 1 | Electronic Skin from High-Throughput Fabrication of Intrinsically Stretchable Lead Zirconate Titanate Elastomer显示文摘Electronic skin made of thin,soft,stretchable devices that can mimic the human skin and reconstruct the tactile sensation and perception offers great opportunities for prosthesis sensing,robotics controlling,and human-machine interfaces.Advanced materials and mechanics engineering of thin film devices has proven to be an efficient route to enable and enhance flexibility and stretchability of various electronic skins;however,the density of devices is still low owing to the limitation in existing fabrication techniques.Here,we report a high-throughput one-step process to fabricate large tactile sensing arrays with a sensor density of 25 sensors/cm^(2) for electronic skin,where the sensors are based on intrinsically stretchable piezoelectric lead zirconate titanate(PZT)elastomer.The PZT elastomer sensor arrays with great uniformity and passive-driven manner enable highresolution tactile sensing,simplify the data acquisition process,and lower the manufacturing cost.The high-throughput fabrication process provides a general platform for integrating intrinsically stretchable materials into large area,high device density soft electronics for the next-generation electronic skin. | Yiming Liu Huanxi Zheng Ling Zhao Shiyuan Liu Kuanming Yao Dengfeng Li Chunki Yiu Shenghan Gao Raudel Avila Pakpong Chirarattananon Lingqian Chang Zuankai Wang Xian Huang Zhaoqian Xie Zhengbao Yang Xinge Yu | 2020 | Research2020，，1： | 11 |
| 2 | Small-volume solution current-time behavior study for application in reverse iontophoresis-based non-invasive blood glucose monitoring显示文摘The reverse iontophoresis-based glucose monitoring circumstance is similar to the small-volume solution in which mass diffusion controls the current response of the electrochemical biosensors.In this study,the law of mass transfer in this type of solution was analyzed and a mathematic model was established to depict the current-time behavior of the fabricated planar electrode used in the non-invasive meter designed by ourselves.A small-volume glucose solution was directly constructed on the electrode to simulate the reverse iontophoresis-based sensing condition.The correctness of the model was demonstrated by chronoamperometry.Animal assay was subsequently carried out to verify the practicality of the model in determination of blood glucose.The results processed by the new method accurately traced the authentic value,confirming the advantage of the new method and the potential in clinical analysis. | CHANG LingQian LIU ChunXiu HE YinZeng XIAO HongHui CAI XinXia | 2011 | Science China Chemistry2011，54，1： | 4 |
| 3 | Micro/nanodevices for assessment and treatment in stomatology and ophthalmology显示文摘Micro/nanodevices have been widely applied for the real-time monitoring of intracellular activities and the delivery of exogenous substances in the past few years.This review focuses on miniaturized micro/nanodevices for assessment and treatment in stomatology and ophthalmology.We first summarize the recent progress in this field by examining the available materials and fabrication techniques,device design principles,mechanisms,and biosafety aspects of micro/nanodevices.Following a discussion of biochemical sensing technology from the cellular level to the tissue level for disease assessment,we then summarize the use of microneedles and other micro/nanodevices in the treatment of oral and ocular diseases and conditions,including oral cancer,eye wrinkles,keratitis,and infections.Along with the identified key challenges,this review concludes with future directions as a small fraction of vast opportunities,calling for joint efforts between clinicians and engineers with diverse backgrounds to help facilitate the rapid development of this burgeoning field in stomatology and ophthalmology. | An’an Sheng Long Lin Jia Zhu Jian Zhuang Jian Li Lingqian Chang Huanyu Cheng | 2021 | Microsystems & Nanoengineering2021，7，1： | 3 |
| 4 | On-chip multiplexed single-cell patterning and controllable intracellular delivery显示文摘Conventional electroporation approaches show limitations in the delivery of macromolecules in vitro and in vivo.These limitations include low efficiency,noticeable cell damage and nonuniform delivery of cells.Here,we present a simple 3D electroporation platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules.A pyramid pit micropore array chip was fabricated based on a silicon wet-etching method.A controllable vacuum system was adopted to trap a single cell on each micropore.Using this chip,safe single-cell electroporation was performed at low voltage.Cargoes of various sizes ranging from oligonucleotides(molecular beacons,22 bp)to plasmid DNA(CRISPR-Cas9 expression vectors,>9 kb)were delivered into targeted cells with a significantly higher transfection efficiency than that of multiple benchmark methods(e.g.,commercial electroporation devices and Lipofectamine).The delivered dose of the chemotherapeutic drug could be controlled by adjusting the applied voltage.By using CRISPR-Cas9 transfection with this system,the p62 gene and CXCR7 gene were knocked out in tumor cells,which effectively inhibited their cellular activity.Overall,this vacuum-assisted micropore array platform provides a simple,efficient,high-throughput intracellular delivery method that may facilitate on-chip cell manipulation,intracellular investigation and cancer therapy. | Zaizai Dong Yanli Jiao Bingteng Xie Yongcun Hao Pan Wang Yuanyuan Liu Junfeng Shi Chandani Chitrakar Stephen Black Yu-Chieh Wang LJames Lee Mo Li Yubo Fan Lingqian Chang | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
| 5 | Multidrug dissolvable microneedle patch for the treatment of recurrent oral ulcer显示文摘Recurrent oral ulcer is a painful oral mucosal disorder that affects 20%of the world’s population.The lack of a radical cure due to its unknown underlying cause calls for innovative symptomatic treatments.This work reports a hyaluronic acid-based dissolvablemicroneedle patch(ROUMNpatch,short for recurrent oral ulcer microneedle)loaded with dexamethasone acetate,vitamin C and tetracaine hydrochloride for the treatment of recurrent oral ulcers.The ROUMN patch shows enhancement in both the anti-inflammatory effect elicited by dexamethasone and the pro-proliferation effect of vitamin C.In vitro experiments show that ROUMN has a higher efficiency in suppressing lipopolysaccharide(LPS)-induced interleukin-6(IL-6)expression than dexamethasone alone.Cell proliferation and migrationwere also significantly promoted byROUMNcompared to vitamin C alone.The healing-promoting effect of ROUMN was also verified in vivo using an acetic acid-cauterized oral ulcer model in rats.ROUMN as a treatment accelerated the healing process of oral ulcers,shortening the total healing time to 5 days compared with the 7 days required by treatment using watermelon frost,a commonly used over-the-counter(OTC)drug for oral ulcers.The rapid dissolution of the hyaluronic acid-based microneedles and the superior healing-promoting effect of the drug combination could lead to a broad application prospect of the ROUMN patch in the treatment of recurrent oral ulcers. | Yuqiong Wang An’an Sheng Xinran Jiang Shanshan Yang Long Lin Mingzhu Yang Fengshuo Zhu Yongyan Hu Jian Li Lingqian Chang | 2023 | Bio-Design and Manufacturing2023，6，3： | 1 |
| 6 | Current research status of tumor cell biomarker detection显示文摘With the annual increases in the morbidity and mortality rates of tumors,the use of biomarkers for early diagnosis and real-time monitoring of tumor cells is of great importance.Biomarkers used for tumor cell detection in body fluids include circulating tumor cells,nucleic acids,protein markers,and extracellular vesicles.Among them,circulating tumor cells,circulating tumor DNA,and exosomes have high potential for the prediction,diagnosis,and prognosis of tumor diseases due to the large amount of valuable information on tumor characteristics and evolution;in addition,in situ monitoring of telomerase and miRNA in living cells has been the topic of extensive research to understand tumor development in real time.Various techniques,such as enzyme-linked immunosorbent assays,immunoblotting,and mass spectrometry,have been widely used for the detection of these markers.Among them,the detection of tumor cell markers in body fluids based on electrochemical biosensors and fluorescence signal analysis is highly preferred because of its high sensitivity,rapid detection and portable operation.Herein,we summarize recent research progress in the detection of tumor cell biomarkers in body fluids using electrochemical and fluorescence biosensors,outline the current research status of in situ fluorescence monitoring and the analysis of tumor markers in living cells,and discuss the technical challenges for their practical clinical application to provide a reference for the development of new tumor marker detection methods. | Liying Jiang Xinyi Lin Fenghua Chen Xiaoyun Qin Yanxia Yan Linjiao Ren Hongyu Yu Lingqian Chang ang Wang | 2023 | Microsystems & Nanoengineering2023，9，5： | 0 |
| 7 | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens显示文摘To minimize and control the transmission of infectious diseases,a sensitive,accurate,rapid,and robust assay strategy for application on-site screening is critical.Here,we report single-molecule RNA capture-assisted digital RT-LAMP(SCADL)for point-of-care testing of infectious diseases.Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads,replacing laborious RNA extraction.Droplet generation,amplification,and the recording of results are all integrated on a microfluidic chip.In assaying commercial standard samples,quantitative results precisely corresponded to the actual concentration of samples.This method provides a limit of detection of 10 copies mL−1 for the N gene within 1 h,greatly reducing the need for skilled personnel and precision instruments.The ultrasensitivity,specificity,portability,rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases. | Liying Jiang Xianghao Lan Linjiao Ren Zhiyuan Jin Xuchen Shan Mingzhu Yang Lingqian Chang | 2023 | Microsystems & Nanoengineering2023，9，5： | 0 |
| 8 | Highly integrated watch for noninvasive continual glucose monitoring显示文摘This article reports a highly integrated watch for noninvasive continual blood glucose monitoring.The watch employs a Nafion-coated flexible electrochemical sensor patch fixed on the watchband to obtain interstitial fluid(ISF)transdermally at the wrist.This reverse iontophoresis-based extraction method eliminates the pain and inconvenience that traditional fingerstick blood tests pose in diabetic patients’lives,making continual blood glucose monitoring practical and easy.All electronic modules,including a rechargeable power source and other modules for signal processing and wireless transmission,are integrated onto a watch face-sized printed circuit board(PCB),enabling comfortable wearing of this continual glucose monitor.Real-time blood glucose levels are displayed on the LED screen of the watch and can also be checked with the smartphone user interface.With 23 volunteers,the watch demonstrated 84.34%clinical accuracy in the Clarke error grid analysis(zones A+B).In the near future,commercial products could be developed based on this lab-made prototype to provide the public with noninvasive continual glucose monitoring. | Tianrui Chang Hu Li Nianrong Zhang Xinran Jiang Xinge Yu Qingde Yang Zhiyuan Jin Hua Meng Lingqian Chang | 2022 | Microsystems & Nanoengineering2022，8，1： | 0 |
| 9 | Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases显示文摘Corona Virus Disease 2019(COVID-19)has developed into a global pandemic in the last two years,causing significant impacts on our daily life in many countries.Rapid and accurate detection of COVID-19 is of great importance to both treatments and pandemic management.Till now,a variety of point-of-care testing(POCT)approaches devices,including nucleic acid-based test and immunological detection,have been developed and some of them has been rapidly ruled out for clinical diagnosis of COVID-19 due to the requirement of mass testing.In this review,we provide a summary and commentary on the methods and biomedical devices innovated or renovated for the quick and early diagnosis of COVID-19.In particular,some of micro and nano devices with miniaturized structures,showing outstanding analytical performances such as ultra-sensitivity,rapidness,accuracy and low cost,are discussed in this paper.We also provide our insights on the further implementation of biomedical devices using advanced micro and nano technologies to meet the demand of point-of-care diagnosis and home testing to facilitate pandemic management.In general,our paper provides a comprehensive overview of the latest advances on the POCT device for diagnosis of COVID-19,which may provide insightful knowledge for researcher to further develop novel diagnostic technologies for rapid and on-site detection of pathogens including SARS-CoV-2. | Yang Wang Huiren Xu Zaizai Dong Zhiying Wang Zhugen Yang Xinge Yu Lingqian Chang | 2022 | Medicine in Novel Technology and Devices2022，，2： | 0 |
| 10 | Recent electroporation-based systems for intracellular molecule delivery显示文摘Intracellular delivery of functional molecules,such as DNA probes and plasmids,is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.Among various delivery methods,recent years have seen the emergence of electroporation-based techniques that provide versatile platforms for molecule delivery,with high efficiency and controlled dosage.In this Review,we describe recent electroporation-based systems for intracellular molecule delivery.The principles of electroporation for cell membrane perforation and cargo delivery are briefly summarized.Focusing on various scenarios for the application of electroporation,we review electroporation devices that variously employ structures based on nanochannels,nanostraws,and flow-through microfluidic channels for in vitro intracellular molecule delivery.We also consider in vivo targeted therapies based on delivery of active molecules by electroporation according to the lesion locations.Finally,we discuss the current challenges facing electroporation-based techniques,as well as opportunities for their future development,which may lead to innovations in intracellular molecule delivery both for cellular analysis in the laboratory and treatment in the clinic. | Zaizai Dong Lingqian Chang | 2021 | Nanotechnology and Precision Engineering2021，4，4： | 0 |
| 11 | Low-Cost and Scalable Platform with Multiplexed Microwell Array Biochip for Rapid Diagnosis of COVID-19显示文摘Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19.Here,we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2.The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes.A polymeric chip with a laser-engraved microwell array was developed to process the reaction between the primers and the respiratory swab RNA extracts,based on reverse transcriptase loop-mediated isothermal amplification(RT-LAMP).To achieve clinically acceptable performance,we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2.Clinical trials were conducted with 87 PCR-positive and 43 PCRnegative patient samples.The platform demonstrated both high sensitivity(95.40%)and high specificity(95.35%),showing potentials for rapid and user-friendly diagnosis of COVID-19 among many other infectious pathogens. | Yang Wang Kaiju Li Gaolian Xu Chuan Chen Guiqin Song Zaizai Dong Long Lin Yu Wang Zhiyong Xu Mingxia Yu Xinge Yu Binwu Ying Yubo Fan Lingqian Chang Jia Geng | 2021 | Research2021，，1： | 0 |
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