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| 1 | Antibacterial and angiogenic chitosan microneedle array patch for promoting wound healing显示文摘A patch with the capability of avoiding wound infection and promoting tissue remolding is of great value for wound healing.In this paper,we develop a biomass chitosan microneedle array(CSMNA)patch integrated with smart responsive drug delivery for promoting wound healing.Chitosan possesses many outstanding features such as the natural antibacterial property and has been widely utilized for wound healing.Besides,the microstructure of microneedles enables the effective delivery of loaded drugs into the target area and avoids the excessive adhesion between the skin and the patch.Also,vascular endothelial growth factor(VEGF)is encapsulated in the micropores of CSMNA by temperature sensitive hydrogel.Therefore,the smart release of the drugs can be controllably realized via the temperature rising induced by the inflammation response at the site of wounds.It is demonstrated that the biomass CSMNA patch can promote inflammatory inhibition,collagen deposition,angiogenesis,and tissue regeneration during the wound closure.Thus,this versatile CSMNA patch is potentially valuable for wound healing in clinical applications. | Junjie Chi Xiaoxuan Zhang Canwen Chen Changmin Shao Yuanjin Zhao Yongan Wang | 2020 | Bioactive Materials2020，5，2： | 25 |
| 2 | Chinese herb microneedle patch for wound healing显示文摘Traditional Chinese medicine and Chinese herbs have a demonstrated value for disease therapy and sub-health improvement.Attempts in this area tend to develop new forms to make their applications more convenient and wider.Here,we propose a novel Chinese herb microneedle(CHMN)patch by integrating the herbal extracts,Premna microphylla and Centella asiatica,with microstructure of microneedle for wound healing.Such path is composed of sap extracted from the herbal leaves via traditional kneading method and solidified by plant ash derived from the brine induced process of tofu in a well-designed mold.Because the leaves of the Premna microphylla are rich in pectin and various amino acids,the CHMN could be imparted with medicinal efficacy of heat clearing,detoxicating,detumescence and hemostatic.Besides,with the excellent pharmaceutical activity of Asiatic acid extracted from Centella asiatica,the CHMN is potential in promoting relevant growth factor genes expression in fibroblasts and showing excellent performance in anti-oxidant,anti-inflammatory and anti-bacterial activity.Taking advantages of these pure herbal compositions,we have demonstrated that the derived CHMN was with dramatical achievement in anti-bacteria,inhibiting inflammatory,collagen deposition,angiogenesis and tissue reconstruction during the wound closure.These results indicate that the integration of traditional Chinese herbs with progressive technologies will facilitate the development and promotion of traditional Chinese medicine in modern society. | Junjie Chi Lingyu Sun Lijun Cai Lu Fan Changmin Shao Luoran Shang Yuanjin Zhao | 2021 | Bioactive Materials2021，6，10： | 8 |
| 3 | Tofu-inspired microcarriers from droplet microfluidics for drug delivery显示文摘Microcarriers have attracted increasing interests in drug delivery. In order to develop this technique, it is prone to focus on the generation of functional particles through using simple approaches and novel but accessible materials. Here, inspired by the formation mechanism of tofu that through the mixing of soymilk and brine for cross-linking soybean proteins, we present novel soybean protein microcarriers by using microfluidic generation approach for drug delivery. Since the soybean protein droplets are generated by microfluidic emulsification method, the tofu microparticles present highly monodisperse and homogeneous morphologies. Because of the excellent biocompatibility of the soybean protein and the interconnected porous structures throughout the whole microparticles after freeze-drying, various kinds of drugs and active molecules could be absorbed and loaded in the microcarriers, which makes them versatile for drug delivery. It can be anticipated that the microfluidic-generated tofu microcarriers will have great potential in the biomedical field. | Han Zhang Yuxiao Liu Jie Wang Changmin Shao Yuanjin Zhao | 2019 | Science China Chemistry2019，62，1： | 5 |
| 4 | Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture显示文摘Three-dimensional(3D)porous scaffolds have a demonstrated value for tissue engineering and regenerative medicine.Inspired by the predation processes of marine predators in nature,we present new photocontrolled shrinkable inverse opal graphene oxide(GO)hydrogel scaffolds for cell enrichment and 3D culture.The scaffolds with adjustable pore sizes and morphologies were created using a GO and N-isopropylacrylamide dispersed solution as a continuous phase of microfluidic emulsions for polymerizing and replicating.Because of the interconnected porous structures and the remotely controllable volume responsiveness of the scaffolds,the suspended cells could be enriched into the inner spaces of the scaffolds through predator-like swallowing and discharging processes.Hepatocyte cells concentrated in the scaffold pores could form denser 3D spheroids more quickly via the controlled compression force caused by the shrinking of the dynamic scaffolds.More importantly,with a program of scaffold enrichment with different cells,an unprecedented 3D multilayer coculture system of endothelial-cellencapsulated hepatocytes and fibroblasts could be generated for applications such as liver-on-a-chip and bioartificial liver.It was demonstrated that the resultant multicellular system offered significant improvements in hepatic functions,such as albumin secretion,urea synthesis,and cytochrome P450 expression.These features of our scaffolds make them highly promising for the biomimetic construction of various physiological and pathophysiological 3D tissue models,which could be used for understanding tissue level biology and in vitro drug testing applications. | Changmin Shao Yuxiao Liu Junjie Chi Jie Wang Ze Zhao Yuanjin Zhao | 2019 | Research2019，，1： | 4 |
| 5 | Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation显示文摘Drug resistance is one of the major obstacles in the drug therapy of cancers.Efforts in this area in pre-clinical research have focused on developing novel platforms to evaluate and decrease drug resistance.In this paper,inspired by the structure of hives where swarms live and breed,we propose porous hydrogel arrays with a uniform pore structure for the generation of hepatoma cell spheroids and the investigation of drug resistance.The porous hydrogel arrays were fabricated using polyeth-ylene glycol diacrylate(PEGDA)hydrogel to negatively replicate a well-designed template.Benefiting from the elaborate processing of the template,the prepared porous hydrogel arrays possessed a uniform pore structure.Due to their anti-adhesion properties and the excellent biocompatibility of the PEGDA hydrogel,the hepatoma cells could form well-defined and uni-form hepatoma cell spheroids in the porous hydrogel arrays.We found that the resistant hepatoma cell spheroids showed more significant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional(2D)cell culture,which reveals the reason for the failure of most 2D cell-selected drugs for in vivo applications.These features give such porous hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and in vitro drug resistance. | Xin Lei Changmin Shao Xin Shou Keqing Shi Liang Shi Yuanjin Zhao | 2021 | Bio-Design and Manufacturing2021，4，4： | 1 |
| 6 | Hierarchical Hydrogels with Ordered Micro-Nano Structures for Cancer-on-a-Chip Construction显示文摘In the drug therapy of tumor,efficient and stable drug screening platforms are required since the drug efficacy varies individually.Here,inspired by the microstructures of hepatic lobules,in which hepatocytes obtain nutrients from both capillary vessel and the central vein,we present a novel hierarchical hydrogel system with ordered micro-nano structure for liver cancer-on-a-chip construction and drug screening.The hierarchical hydrogel system was fabricated by using pregel to fill and replicate selfassembled colloidal crystal arrays and microcolumn array template.Due to the synergistic effect of its interconnected micro-nano structures,the resultant system could not only precisely control the size of cell spheroids but also realize adequate nutrient supply of cell spheroids.We have demonstrated that by integrating the hierarchical hydrogel system into a multichannel concentration gradients microfluidic chip,a functional liver cancer-on-a-chip could be constructed for high-throughput drug screening with good repeatability and high accuracy.These results indicated that the hierarchical hydrogel system and its derived liver cancer-on-a-chip are ideal platforms for drug screening and have great application potential in the field of personalized medicine. | Luyao Zhu Changmin Shao Hanxu Chen Zhuoyue Chen Yuanjin Zhao | 2021 | Research2021，，1： | 1 |
| 7 | Hierarchical Hydrogels with Ordered Micro-Nano Structures for Cancer-on-a-Chip Construction显示文摘In the drug therapy of tumor,efficient and stable drug screening platforms are required since the drug efficacy varies individually.Here,inspired by the microstructures of hepatic lobules,in which hepatocytes obtain nutrients from both capillary vessel and the central vein,we present a novel hierarchical hydrogel system with ordered micro-nano structure for liver cancer-on-a-chip construction and drug screening.The hierarchical hydrogel system was fabricated by using pregel to fill and replicate self-assembled colloidal crystal arrays and microcolumn array template.Due to the synergistic effect of its interconnected micro-nano structures,the resultant system could not only precisely control the size of cell spheroids but also realize adequate nutrient supply of cell spheroids.We have demonstrated that by integrating the hierarchical hydrogel system into a multichannel concentration gradients microfluidic chip,a functional liver cancer-on-a-chip could be constructed for high-throughput drug screening with good repeatability and high accuracy.These results indicated that the hierarchical hydrogel system and its derived liver cancer-on-a-chip are ideal platforms for drug screening and have great application potential in the field of personalized medicine. | Luyao Zhu Changmin Shao Hanxu Chen Zhuoyue Chen Yuanjin Zhao | 2022 | Research2022，，2： | 0 |
| 8 | Hierarchically Inverse Opal Porous Scaffolds from Droplet Microfluidics for Biomimetic 3D Cell Co-Culture显示文摘With the advantages of better mimicking the specificity of natural tissues,three-dimensional(3D)cell culture plays a major role in drug development,toxicity testing,and tissue engineering.However,existing scaffolds or microcarriers for 3D cell culture are often limited in size and show suboptimal performance in simulating the vascular complexes of living organisms.Therefore,we present a novel hierarchically inverse opal porous scaffold made via a simple microfluidic approach for promoting 3D cell co-culture techniques.The designed scaffold is constructed using a combined concept involving an emulsion droplet template and inert polymer polymerization.This work demonstrates that the resultant scaffolds ensure a sufficient supply of nutrients during cell culture,so as to achieve large-volume cell culture.In addition,by serially planting different cells in the scaffold,a 3D co-culture system of endothelial-cellencapsulated hepatocytes can be developed for constructing certain functional tissues.It is also demonstrated that the use of the proposed scaffold for a co-culture system helps hepatocytes to maintain specific in vivo functions.These hierarchically inverse opal scaffolds lay the foundation for 3D cell culture and even the construction of biomimetic tissues. | Changmin Shao Yuxiao Liu Junjie Chi Fangfu Ye Yuanjin Zhao | 2021 | Engineering2021，7，12： | 0 |
| 9 | Living Materials for Life Healthcare显示文摘CONSPECTUS:Living material is a type of composite material with living elements integrated into nonliving components.It combines the advantages of both living cells and nonliving substances and takes advantage of advanced fabrication techniques.Living materials is an emerging new paradigm at the frontiers of materials research.It addresses an intriguing question of how to let materials show life-like capabilities and function in an integrative and programmable way.Of all of the capability and application aspects of living materials,the one related to human health is the most intriguing since it implies that that materials could be made by life and used for life.Specifically,living materials exploit the merits of cells in that they can respond to environmental variables,synthesize functional molecules,and span multiple length scales.At the same time,the abundant coordination between live and nonliving elements opens infinite possibilities for diverse structural and functional features.The resultant biohybrid materials are thus endowed with novel functions that recapitulate or even surpass their natural candidates and ultimately shed new light on biodiagnostics and biotherapeutics to address human health concerns.In this Account,we present a concise summary and analysis of living materials and bring it into the context of addressing life healthcare concerns.We start with the fabrication of living materials,including the engineering of living elements and the manufacturing of biohybrid composites through advanced technologies,particularly micropatterning,microfluidics,and threedimensional(3D)printing.We then shift to the properties and capacities of living materials,such as autonomous motion,sensing and actuation,and highly sensitive detection.On the basis of these,we show,by representative examples,the healthcare-oriented applications of living materials in both diagnostic and biotherapeutic aspects,including biohybrid sensors and actuators,cell-powered robotics,organ-on-a-chip platforms,wearable devices,smart delivery vehicles,cell therapy,and tissue engineering.Despite the exciting achievements of living materials,challenges remain in the precise manipulation of the properties of living materials;therefore,we provide our insight for future directions in this burgeoning field with respect to both fundamental research and technical innovation.The former concerns a comprehensive understanding of spatiotemporal coordination between living and nonliving components as well as the heterogeneous properties of the biohybrid materials.The latter looks at precise gene editing of living elements,multiscale manufacturing of cell-involved entities,and the incorporation of signal-transducing units for creating advanced living devices.With extensive multidisciplinary cooperation in the aforementioned areas,we expect living materials to stand out at the forefront of material science and engineering and take a giant leap forward to improve human health. | Luoran Shang Changmin Shao Junjie Chi Yuanjin Zhao | 2021 | Accounts of Materials Research2021，2，1： | 0 |
| 10 | Construction and application of liver cancer models in vitro显示文摘Primary liver cancer is the fifth most common malignancy and the third leading cause of cancer death worldwide.Although current advances in the treatment of liver cancer,the prognosis of this cancer remains unfavorable.Appropriate liver cancer model in vitro is an important way to study the pathogenesis and drug screening of liver cancer.This review provides a comprehensive summary and discussion on the construction and application of liver cancer models in vitro,in particular hepatocellular carcinoma(HCC).Specifically,after introducing the current methods or techniques for preparing 3D in vitro liver cancer models,this review summarizes the relevant applications of these liver cancer models in vitro,e.g.drug screening,personalized medicine,and other applications.In the end,this review discusses the advantages and disadvantages of the liver cancer models in vitro,and proposes future prospects and research directions. | Changmin Shao Qingfei Zhang Gaizhen Kuang Qihui Fan Fangfu Ye | 2022 | Engineered Regeneration2022，3，3： | 0 |
| 11 | Microfluidics for Medical Additive Manufacturing显示文摘Additive manufacturing plays a vital role in the food,mechanical,pharmaceutical,and medical fields.Within these fields,medical additive manufacturing has led to especially obvious improvements in medical instruments,prostheses,implants,and so forth,based on the advantages of cost-effectiveness,customizability,and quick manufacturing.With the features of precise structural control,high throughput,and good component manipulation,microfluidic techniques present distinctive benefits in medical additive manufacturing and have been applied in the areas of drug discovery,tissue engineering,and organs on chips.Thus,a comprehensive review of microfluidic techniques for medical additive manufacturing is useful for scientists with various backgrounds.Herein,we review recent progress in the development of microfluidic techniques for medical additive manufacturing.We evaluate the distinctive benefits associated with microfluidic technologies for medical additive manufacturing with respect to the fabrication of droplet/fiber templates with different structures.Extensive applications of microfluidic techniques for medical additive manufacturing are emphasized,such as cell guidance,three-dimensional(3D)cell culture,tissue assembly,and cell-based therapy.Finally,we present challenges in and future perspectives on the development of microfluidics for medical additive manufacturing. | Jie Wang Changmin Shao Yuetong Wang Lingyun Sun Yuanjin Zhao | 2020 | Engineering2020，6，11： | 0 |
| 12 | Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation显示文摘SARS-CoV-2 has caused asevere pneumoniapandemic worldwide with high morbidity and mortality.Howto developa preclinical model for recapitulating SARS-CoV-2 pathogenesis is stil urgent and essential for the control of the pandemic.Here,we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration.We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar units,which lays a fundamental basis for viral infection studies at the organ level.Using virus-analogous chemicals and pseudovirus,we have explored virus pathogenesis and blocking ability of antibodies during viral infection.This work provides a favorable platform for SARS-Cov-2-related researches and has a great potential for physiology and pathophysiology studies of the human lung at the organ level in vitro. | Ting Cao Changmin Shao Xiaoyu Yu Ruipei Xie Chen Yang Yulong Sun Shaohua Yang Wangjian He Ye Xu Qihui Fan Fangfu Ye | 2022 | Research2022，，2： | 0 |
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