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| 1 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 2 | Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells显示文摘瞄准:为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀,区分了 hepatocytic 房间。方法:MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF ), hepatocyte 生长因素( HGF ),表皮的生长因素( EGF ),和成纤维细胞生长因素 4 ( FGF-4 )独自一个,或在刚孤立的 FLC 的存在。在合作文化的房间被收获,并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ,白朊,和 alpha-fetoprotein 在不同房间人口被执行。结果:在指定文化条件下面,与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊, CK-18,和 AFP-RNA。在 wk 3, MSC 失去了 hepatocytic 基因表示,可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论:从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间,在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。 | Claudia Lange Helge Bruns Dietrich Kluth Axel R Zander Henning C Fiegel | 2006 | World Journal of Gastroenterology2006，12，15： | 23 |
| 3 | Functions of Chloroplastic Adenylate Kinases in Arabidopsis显示文摘 | PETER ROBERT LANGE CLAUDIA GESERICK GILBERT TISCHENDORF | | 0，，： | 1 |
| 4 | An ecologically-based method for selecting ecological indicators for assessing risks to biological diversity from genetically-engineered plants显示文摘The environmental risks associated with genetically-engineered( GE) organisms have been controversial,and so have the models for the assessment of these risks. We propose an ecologically-based environmental risk assessment( ERA) model that follows the 1998 USEPA guidelines,focusing on potential adverse effects to biological diversity. The approach starts by( 1) identifying the local environmental values so the ERA addresses specific concerns associated with local biological diversity. The model simplifies the indicator endpoint selection problem by( 2) classifying biological diversity into ecological functional groups and selecting those that deliver the identified environmental values.( 3) All of the species or ecosystem processes related to the selected functional groups are identified and( 4) multi-criteria decision analysis( MCDA) is used to rank the indicator endpoint entities,which may be species or ecological processes. MCDA focuses on those species and processes that are critical for the identified ecological functions and are likely to be highly exposed to the GE organism. The highest ranked indicator entities are selected for the next step.( 5) Relevant risk hypotheses are identified. Knowledge about the specific transgene and its possible environmental effects in other countries can be used to assist development of risk hypotheses.( 6) The risk hypotheses are ranked using MCDA with criteria related to the severity of the potential risk. The model emphasizes transparent,expert-driven,ecologically-based decision-making and provides formal methods for completing a screening level-ERA that can focus ERA on the most significant concerns. The process requires substantial human input but the human capital is available in most countries and regions of the world. | D.A.Andow Gabor L.Lvei Salvatore Arpaia Lewis Wilson Eliana M.G.Fontes Angelika Hilbeck Andreas Lang Nguyên Vǎn Tu't C.S.S.Pires E.R.Sujii Claudia Zwahlen A.N.E.Birch Deise M.F.Capalbo Kristina Prescott Celso Omoto Adam R.Zeilinger | 2013 | 生物安全学报2013，22，3： | 1 |
| 5 | Size abnormalities of the superior parietal cortices are related to dissociation in borderline personality disorder显示文摘 | Eva Irle Claudia Lange Godehard Weniger Ulrich Sachsse | 2007 | Psychiatry Research: Neuroimaging2007，，2： | 1 |
| 6 | Loss of PSP94 expression is associated with early PSA recurrence and deteriorates outcome of PTEN deleted prostate cancers显示文摘Objective: Prostate secretory protein of 94 amino acids(PSP94) is a target gene of the EZH2 transcriptional repressor and is often downregulated in prostate cancer;however, its prognostic value is disputed.Methods: Immunohistochemical analysis of a tissue microarray of 12, 432 prostate cancer specimens was performed to evaluate PSP94 expression. Correlation of PSP94 expression with tumor phenotype, patient prognosis, TMPRSS2:ERG fusion status, EZH2 expression and PTEN deletion was studied.Results: PSP94 expression was increased in benign prostatic hyperplasia;however, it was downregulated in 48% and negative in42% of the 9, 881 interpretable prostate cancer specimens. The loss of PSP94 expression was inversely correlated to EZH2 expression(P < 0.0001) and largely unrelated to the ERG status, but strongly correlated with high Gleason grade, advanced tumor stage, and nodal metastasis(P <0.0001 each). The fraction of PSP94-negative cancer specimens increased from 40% in pT2 to 52%in pT3 b-pT4(P < 0.0001) and from 40% in Gleason 3+3 = 6 to 46% in Gleason 4+3 = 7 and 60% in Gleason ≥4+4 = 8(P <0.0001). Loss of PSP94 was linked to early prostate-specific antigen recurrence, but with little absolute effect(P < 0.0001).However, it provided additional prognostic impact in cancer specimens with PTEN deletion. Loss of PSP94 deteriorated prognosis of cancer patients with PTEN deletion by more than 10%(P < 0.0001). The combination of PTEN deletion and PSP94 loss provided independent prognostic information that was observed in several subgroups defined by classical and quantitative Gleason grade.Conclusions: The results of our study suggest that combined PSP94/PTEN analysis can be potentially used in the clinical prognosis of prostate cancer. | Andreas M. Luebke Ali Attarchi-Tehrani Jan Meiners Claudia Hube-Magg Dagmar S. Lang Martina Kluth Maria Christina Tsourlakis Sarah Minner Ronald Simon Guido Sauter Franziska Buscheck Frank Jacobsen Andrea Hinsch Stefan Steurer Thorsten Schlomm Hartwig Huland Markus Graefen Alexander Haese Hans Heinzer Till S. Clauditz Eike Burandt Waldemar Wilczak Doris Hoflmayer | 2019 | Cancer Biology & Medicine2019，16，2： | 1 |
| 7 | Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use显示文摘 | Norbert Stute Katja Holtz Michael Bubenheim Claudia Lange Felix Blake Axel R. Zander | 2004 | Experimental Hematology2004，，12： | 1 |
| 8 | Success through diversity – How Staphylococcus epidermidis establishes as a nosocomial pathogen显示文摘 | Sonja M.K. Schoenfelder Claudia Lange Martin Eckart Susanne Hennig Svitlana Kozytska Wilma Ziebuhr | 2010 | International Journal of Medical Microbiology2010，，6： | 1 |
| 9 | The self and social cognition: the role of cortical midline structures and mirror neurons显示文摘 | Lucina Q. Uddin Marco Iacoboni Claudia Lange Julian Paul Keenan | 2007 | Trends in Cognitive Sciences2007，，4： | 1 |
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