| 1 | Analysis on virus resistance and fruit quality for T4 generation of transgenic papaya显示文摘Molecular biological characterization,fruit char-acters,and nutrients were analyzed for T4 generation of transgenic papaya.All transgenic papaya plants with the mutated replicase(RP)gene from papaya ringspot virus(PRSV)showed high resistance or immunity against PRSV in the field.The RP transgene can be steadily inherited to,and expressed at RNA level,the progenies.The growth char-acteristics of transgenic papaya were much better than non-transgenic papaya in the field.The non-transgenic papaya seedlings began to show typical symptoms caused by PRSV after being inoculated with PRSV.They died quickly and never grew to produce fruit.The adult trees developed yellow leaves and produced smaller fruits and were doomed to a slow death after some time,while most of transgenic papaya plants(about 91.8%)did not show any symptoms caused by PRSV,and produced more,bigger,and high quality fruits.Compared with non-transgenic plants,the fresh fruit length of T4 gen-eration of transgenic papaya increased 2.6%-5%,and the diameter decreased 0.6%-1.5%.The flesh thickness of fresh fruit increased 12%-15%,which made it fitter for eating.Although the fresh fruit quality changed,there was no sig-nificant difference between transgenic and non-transgenic papaya.The quality characteristics of dry fruit including the contents of water,lipid,N,protein,reduced sugar,vitamin A,vitamin C,and carotene in the T4 generation of transgenic papaya were all the same as their non-transgenic parents.This means that transgenic plants and non-transgenic plants are substantially equivalent,and the transgene has no effect on dry fruit quality.In this study,we found that vitamin A and vitamin C in red-fleshed papaya were 1.4-1.8 and 1.78-2.07 times more than the yellow-fleshed ones,respectively,while N and protein were only 84.2%-92.1%and 82.1%-98.9%of the yellow-fleshed ones. | WEI Xiangdong LAN Congyu LU Zhijing YE Changming | 2007 | Frontiers in Biology2007，2，3： | 1 |
| 2 | Computational Viromics: Applications of the Computational Biology in Viromics Studies显示文摘Viruses are a kind of biological entities which rely on host cells for survival.Depending on the genetic materials and replication mode,they can be grouped into double-stranded DNA(dsDNA),single-stranded DNA(ssDNA),doublestranded RNA(dsRNA),positive-sense single-stranded RNA(+ssRNA),negative-sense single-stranded RNA(-ssRNA),ssRNA reverse transcriptase viruses(ssRNART)and dsDNA reverse transcriptase viruses(dsDNA-RT)(Walker et al.2020).Viruses can infect most kinds of biological entities,including viruses,bacteria,archaea and eukaryote(La Scola et al.2008;Fermin,2018). | Congyu Lu Yousong Peng | 2021 | Virologica Sinica2021，36，5： | 0 |
| 3 | PREDAV-H1: a user-friendly web server for predicting antigenic variants of influenza H1N1 viruses显示文摘Dear Editor,The influenza H1N1 virus has caused three global pandemics since the beginning of the 20th century(Liu et al.,2015).The first is the notorious Spanish flu in 191&which killed 20-100 million people in the world.It circulated for nearly 40 years and was replaced by influenza H2N2 virus.In 1977,the virus reappeared in Russia and caused global pandemics.It continued to circulate until 2009 when it was replaced by the pandemic H1N1 virus.Influenza H1N1 viruses cause large morbidity and mortality to human society,and will continue to threaten humans.Vaccination is the most effective way to fight against the virus.However,due to rapid mutation of the virus,antigenic drift happens frequently,which leads to inefficiency of influenza vaccines.How to timely identify antigenic variants is an important question in influenza surveillance. | Congyu Lu Mi Liu Aiping Wu Yuelong Shu Yousong Peng Taijiao Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 0 |
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