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| 1 | Overexpression of phosphatidylserine synthase IbPSS1 affords cellular Na+homeostasis and salt tolerance by activating plasma membrane Na^(+)/H^(+)antiport activity in sweet potato roots显示文摘Phosphatidylserine synthase(PSS)-mediated phosphatidylserine(PS)synthesis is crucial for plant development.However,little is known about the contribution of PSS to Na^(+)homeostasis regulation and salt tolerance in plants.Here,we cloned the IbPSS1 gene,which encodes an ortholog of Arabidopsis AtPSS1,from sweet potato(Ipomoea batatas(L.)Lam.).The transient expression of IbPSS1 in Nicotiana benthamiana leaves increased PS abundance.We then established an efficient Agrobacterium rhizogenes-mediated in vivo root transgenic system for sweet potato.Overexpression of IbPSS1 through this system markedly decreased cellular Na^(+)accumulation in salinized transgenic roots(TRs)compared with adventitious roots.The overexpression of IbPSS1 enhanced salt-induced Na^(+)/H^(+)antiport activity and increased plasma membrane(PM)Ca^(2+)-permeable channel sensitivity to NaCl and H2O2 in the TRs.We confirmed the important role of IbPSS1 in improving salt tolerance in transgenic sweet potato lines obtained from an Agrobacterium tumefaciens-mediated transformation system.Similarly,compared with the wild-type(WT)plants,the transgenic lines presented decreased Na^(+)accumulation,enhanced Na^(+)exclusion,and increased PM Ca^(2+)-permeable channel sensitivity to NaCl and H2O2 in the roots.Exogenous application of lysophosphatidylserine triggered similar shifts in Na^(+)accumulation and Na^(+)and Ca^(2+)fluxes in the salinized roots of WT.Overall,this study provides an efficient and reliable transgenic method for functional genomic studies of sweet potato.Our results revealed that IbPSS1 contributes to the salt tolerance of sweet potato by enabling Na^(+)homeostasis and Na^(+)exclusion in the roots,and the latter process is possibly controlled by PS reinforcing Ca^(2+)signaling in the roots. | Yicheng Yu Ying Xuan Xiaofeng Bian Lei Zhang Zhiyuan Pan Meng Kou Qinghe Cao Zhonghou Tang Qiang Li Daifu Ma Zongyun Li Jian Sun | 2020 | Horticulture Research2020，7，1： | 2 |
| 2 | TPC in the leaves of 116 sweet potato ( Ipomoea batatas L.) varieties and Pushu 53 leaf extracts显示文摘 | Wenqing Xu Lixiang Liu Bing Hu Yi Sun Hong Ye Daifu Ma Xiaoxiong Zeng | 2010 | Journal of Food Composition and Analysis2010，，6： | 1 |
| 3 | Inheritance Tendency of Storage Root Quality Traits in F_1 Hybrids of ‘Xushu 25'and ‘Xu 22-5'显示文摘‘Xushu 25',‘Xu 22-5'and their reciprocal F1 hybrids were used as experimental materials to analyze the inheritance tendency of storage root quality traits of sweetpotato. The results showed that most of the storage root traits in F1 hybrids of‘Xushu 25'and‘Xu 22-5'were quantitative traits controlled by multiple genes. The carotenoid content in fresh storage roots of F1 hybrids was lower than that of parents,with the average genetic transmitting ability and average coefficient of variation at 51. 17% and 103. 21%,respectively. The starch content in fresh storage roots reached mid-parent level,with the average genetic transmitting ability at 96. 66%. The reducing sugar content in fresh storage roots of F1 hybrids tended to be polarized; to be specific,most of the F1 hybrids exhibited higher reducing sugar content than their parents with high reducing sugar content or exhibited lower reducing sugar content than their parents with low reducing sugar content,but F1 hybrids with reducing sugar content ranging between that of their parents accounted for a small proportion. The soluble sugar content in fresh storage roots reached mid-parent level and tended to be lower than parents,with the average genetic transmitting ability and average coefficient of variation at 82. 25% and21. 93%,respectively. The crude protein content in fresh storage roots of F1 hybrids exceeded mid-parent level and tended to increase; positive-crossing and negative-crossing progenies exhibiting higher crude protein content than their parents with high crude protein content accounted for 15. 53% and 16. 28%,respectively. | Meng KOU Qiang LI Yungang ZHANG Xin WANG Zhonghou TANG Wei TANG Xiuying LI Daifu MA | 2014 | Agricultural Biotechnology2014，3，6： | 0 |
| 4 | Breeding and Cultivation Techniques of a New Table Use Purple-fleshed Sweetpotato Cultivar,Xuzishu 2显示文摘Xuzishu 2 is a new high-quality table use purple-fleshed sweetpotato cultivar bred from hybrid progenies of AIS35-2 × Xushu 22-5 by Jiangsu Xuzhou Sweetpotato Research Center. In the sweetpotato variety regional trial of Jiangsu Province during 2007- 2008,average fresh storage root yield was 30 185. 3kg / hm2; average dry matter content was 29. 26%; average dry storage root yield was 8 868. 7 kg/hm2. In the sweetpotato variety production test of Jiangsu Province,average fresh storage root yield was 30 683. 9 kg / hm2; average dry storage root yield was 9 399. 0 kg/hm2. Multi-location sampling analysis in the regional trial indicated that the anthocyanin content in fresh storage roots was 104. 6 μg / g. Xuzishu 2 was approved by Crop Variety Approval Committee of Jiangsu Province in March 2010,which exhibited high fresh storage root yield,sweet taste,high anthocyanin content,good commodity,root rot resistance,and was tolerant to moisture and easy to store. Therefore,Xuzishu 2 is a high-quality purple-fleshed sweetpotato variety suitable for consumption and food processing,which should be cultivated in medium- and high-fertility fields with management procedures of suitable high density,early planting and early harvest. | Qiang LI Yiping XIE Xin WANG Xiuying LI Hongmin LI Daifu MA Zhonghou TANG Qinghe CAO Meng KOU Yungang ZHANG Wei TANG | 2015 | Agricultural Biotechnology2015，4，4： | 0 |
| 5 | Prediction model of microwave calcining of ammonium diuranate using incremental improved back-propagation neural network显示文摘The incremental improved Back-Propagation (BP) neural network prediction model using the Levenberg-Marquardt algorithm based on optimizing theory is put forward,which can solve the problems existing in the process of calcinations for ammonium diuranate (ADU) by microwave heating,such as long testing cycle,high testing quantity,difficulty of optimization for process parameters.Many training data probably were offered by the way of increment batch and the limitation of the system memory could make the training data infeasible when the sample scale was large.The prediction model of the nonlinear system is built,which can effectively predict the experiment of microwave calcining of ADU,and the incremental improved BP neural network is very useful in overcoming the local minimum problem,finding the global optimal solution and accelerating the convergence speed. | Yingwei LI Bingguo LIU Jinhui PENG Wei LI Daifu HUANG Libo ZHANG | 2011 | Acta Metallurgica Sinica(English Letters)2011，24，1： | 0 |
| 6 | Effects of Growth Period on Quality and Tuber Traits of New Sweetpotato Cultivar Xuzishu 8显示文摘Xuzishu 8 is a new cultivated sweetpotato with high anthocyanidin content.The growth period affects its tuber traits and quality.In this study,the changes in sweetpotato shape,number of tubers per plant,rate of big and medium tubers,yield,anthocyanin content and starch content of Xuzishu 8 were investigated under 12 growth periods from 78 to 188 d.The results showed that various traits of Xuzishu 8 were significantly different between different growth periods.The rate of large and medium tubers,the fresh sweetpotato yield,the starch content and the anthocyanin yield increased gradually with the extension of the growth period.The length/width ratio of sweetpotato tubers,the rate of small tubers and the contents of reducing sugar and soluble sugar decreased with the prolongation of the growth period.The correlation analysis showed that the fresh sweetpotato yield was in significant or extremely significant positive correlation with the number of tubers per plant,rate of big and medium tubers,dry matter content,dry yield and starch content.The 148-day growth period treatment exhibited the highest number of tubers per plant,the highest fresh sweetpotato yield and anthocyanin yield reaching 3 927 and 4.25 kg/667 m^2,respectively,as well as the rate of large and medium tubers up to 90%.Therefore,the tubers had good marketability and eating quality. | Hui YAN Meng KOU Wei TANG Yaju LIU Xin WANG Qiang LI Daifu MA Yungang ZHANG | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
| 7 | The trivalent cerium-induced cell death and alteration of ion flux in sweetpotato [Ipomoea batatas(L.) Lam]显示文摘The rare earth element cerium(Ce) in its several forms is extensively utilized in various fields, including nano-technology, agriculture, and the food industry. Due to its increasing unregulated usage, Ce is now a potential source of pollution and toxicity due to its excessive environmental accumulation. Unfortunately, analysis of the toxic effects of Ce in plants is still in its early stages. Herein, we investigated the effects of Ce3+ treatment on development-related indicators in sweetpotato. We found that a low concentration(10 mg/L) slightly improved oxidation resistance, while a high concentration(20-80 mg/L)negatively affected development and photosynthesis and triggered increases in reactive oxygen species(ROS) production, antioxidant enzyme activities, and malondialdehyde(MDA) content. Moreover,elevation and efflux of cytosolic Ca^(2+) and caspase-l-like activity were induced by high-concentration Ce^(3+) treatment. Finally, cell viability decreased as Ce3+ concentration increased. These results suggest that(1) a high Ce3+ concentration(20-80 mg/L) inhibits development and photosynthesis of sweetpotato and induces oxidative damage followed by lipid peroxidation in the root,(2) a caspase-l-like protease is induced by cytosolic Ca^(2+) and ROS overproduction to cause programmed cell death in the root, and(3) a high concentration of Ce3+ could trigger a hypothetical cell death pathway, wherein Ce3+induces ROS production followed by cytosolic Ca^(2+) elevation, which activates caspase-l-like activity,which in turn leads to programmed cell death in the root of sweetpotato. | Jiaojiao Jiang Jianzhong Hu Zeyi Xie Qinghe Cao Daifu Ma Yonghua Han Zongyun Li | 2017 | Journal of Rare Earths2017，35，12： | 0 |
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