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您的检索式：作者名="Danqi Zeng"
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| 1 | Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale,which confers abiotic stress tolerance显示文摘Flavonoids are important bioactive components in Dendrobium officinale,a medicinal orchid.They are involved in many biological activities,including protecting plants against biotic and abiotic stresses.Research on the key genes related to flavonoid biosynthesis in D.officinale is limited.In this study,one of the key flavonoid biosynthesis genes,flavanone 3-hydroxylase(F3H),was characterized from D.officinale.The open reading frame of DoF3H was 1134 bp long and it encoded a 377-amino acid protein.The DoF3H protein showed considerably high homology with F3H proteins from other plant species and shared a common evolutionary ancestor with other F3Hs.DoF3H transcripts were detected in different organs of adult plants and mainly accumulated in flowers,followed by roots,stems and leaves,a pattern that was similar to the content of flavonoids.Recombinant DoF3H protein,which was localized in the cytosol,could convert naringenin to dihydrokaempferol.The mRNA levels of DoF3H were significantly induced by salt and cold stresses.Furthermore,the heterologous expression of DoF3H in Escherichia coli conferred it higher tolerance to salt and cold stresses.These results provide insight into the molecular function of DoF3H in the biosynthesis of flavonoids,and provide a new application for improvement of abiotic tolerance in D.officinale. | Can Si Wei Dong Jaime A.Teixeira da Silva Chunmei He Zhenming Yu Mingze Zhang Lei Huang Conghui Zhao Danqi Zeng Chuanmao Li Shengxiang Qiu Jun Duan | 2023 | Horticultural Plant Journal2023，9，2： | 1 |
| 2 | ERF5 enhances protocorm-like body regeneration via enhancement of STM expression in Dendrobium orchid显示文摘Orchid plants develop protocorms upon germination and produce protocorm-like structures called protocorm-like bodies(PLBs)from protocorms and somatic cells via tissue culture.Protocorm-like bodies have broad technical application potential in the orchid industry and their regeneration is a distinct developmental process in the plant kingdom.However,little is known about this unparalleled developmental program.In this study,we identified a PLB-abundant gene,ethylene response factor(ERF),and a transcription factor named Do ERF5,and determined its important role in PLB regeneration in Dendrobium orchid.Overexpression of Do ERF5 in Dendrobium greatly enhanced the PLB regeneration from PLB and stem explants,and upregulated the expression of WOUND-INDUCED DEDIFFERENTIATION(Do WIND)homologs and SHOOT MERISTEMLESS(Do STM),as well as the genes involved in cytokinin biosynthesis(Do IPT)and the cytokinin response factors(Do ARRs).However,silencing Do ERF5 reduced the regeneration rate of PLBs,and downregulated the expression of Do WIND homologs,Do STM and Do ARRs.We demonstrated that Do ERF5 is directly bound to the Do STM promoter and regulates its expression.In addition,overexpression of Do STM in Dendrobium orchid resulted in favorable regeneration of PLBs.Our results clarify that Do ERF5 regulates the regeneration of PLB by enhancing Do STM expression.Our findings provide new insights into how Do ERF5 mediates PLB regeneration and offers technical potential in improving clonal propagation,preservation,and the bioengineering of orchids. | Danqi Zeng Can Si Mingze Zhang Jun Duan Chunmei He | 2023 | Journal of Integrative Plant Biology2023，65，9： | 0 |
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