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| 1 | Concentrations of heavy metals and polycyclic aromatic hydrocarbons in needles of Masson pine (Pinus massoniana L.) growing nearby different industrial sources显示文摘Emissions from industrial activities pose a serious threat to human health and impose the need for monitoring both inorganic and organic pollutants in industrial areas. We selected Masson pine (Pinus massoniana L.) as potential biomonitor and collected the current (C) and previous year (C+1) needles from three industrial sites dominated by petrochemical, ceramics manufacturing, and iron and steel smelting plants and one remote site to determine heavy metals (Cu, Cd, Pb, Zn, Cr, Ni and Co) and polycyclic aromatic hydrocarbons (PAHs) in unwashed and water-washed needles. Both unwashed and washed C+1 needles showed generally higher concentrations of heavy metals and PAHs than C needles, although the washed needles more clearly spotlighted the accumulation effect of PAHs over exposure time. Water-washing resulted in a significant decrease in needle PAH concentrations with more significant effects shown in C needles. By contrast, needle heavy metal concentrations were much less affected by washing. Although heavy metals and PAHs might differ in adsorption and uptake strategies, their higher concentrations in the needles at the industrial sites indicated conspicuous contamination due to industrial emissions there. The PAH distribution patterns in pine needles accorded with the real types of energy consumption in the study sites and were efficiently used for pinpointing local pollutant sources. | Fangfang Sun Dazhi Wen Yuanwen Kuang Jiong Li Jianli Li Weidong Zuo | 2010 | Journal of Environmental Sciences2010，22，7： | 4 |
| 2 | Detection and identificationof intestinal pathogensin clinical specimensusing DNA microarrays显示文摘 | JIN Dazhi WEN Siyuan CHEN Suhong | 2006 | Molecular and Cellular Probes2006，20，6： | 1 |
| 3 | Detection and identification of intestinal pathogens in clinical specimens using DNA microarrays 显示文摘 | JIN Dazhi WEN Siyuan CHEN Suhong ct al | 2006 | Molecular and Cellular Probes2006，20，6： | 1 |
| 4 | Phosphatase activity in relation to key litter and soil properties in mature subtropical forests in China 显示文摘 | Hou En' qing Chen Chengrong Wen Dazhi | 2015 | Science of the Total Environment2015，5155，16： | 1 |
| 5 | DNA microarrays assay for simultaneous detection and identification of multiple common intestinal pathogens显示文摘 | Jin Dazhi Wen Siyuan Chen Suhong | 2006 | Mol Cellular Probes2006，，20： | 1 |
| 6 | Responses of photosynthetic parameters of Mikania micrantha and Chromolaena odorata to contrasting irradiance and soil moisture显示文摘 | ZHANG Lingling WEN Dazhi FU Shenglei | 2009 | Biologia Plantarum2009，53，3： | 1 |
| 7 | Structural and physiological responses of two invasive weeds, Mikania rnicrantha and Chromolaena odorata, to contrasting light and soil water conditions显示文摘 | Zhang Lingling Wen Dazhi | 2009 | Journal of Plant Research2009，122，： | 1 |
| 8 | Responses of photosynthetic parameters of Mikania micrantha and Chromolaena odorata to contrasting irradiance and soil moisture显示文摘 | Zhang Lingling Wen Dazhi Fu Shenglei | 2009 | Biologia Plantarum2009，53，3： | 1 |
| 9 | Structural and physiological responses of two invasive weeds, Mikania micrantha and Chromolaena odorata, to contrasting light and soil water conditions显示文摘 | ZHANG Lingling WEN Dazhi | 2009 | Journal of Plant Research2009，122，： | 1 |
| 10 | Component Analysis of Shuanghuanglian Freeze-dried Powder and Its Effects on Human Hepatocyte Function when Combined with Cefradine显示文摘[Objectives]To detect the contents of components in Shuanghuanglian freeze-dried powder and to explore the effects of Shuanghuanglian freeze-dried powder and its components on human hepatocytes(HL-7702)alone or in combination with cefradine.[Methods]High performance liquid chromatography(HPLC)was used to detect the contents of baicalin,wogonin,chlorogenic acid and forsythin,the main components of Shuanghuanglian freeze-dried powder.HL-7702 cells were cultured with Shuanghuanglian freeze-dried powder and the main components of Shuanghuanglian freeze-dried powder alone or in combination with cefradine.Enzyme linked immunosorbent assay(ELISA)was used to detect the contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the cell supernatant after culture,and HPLC was used to detect the expression level of adenosine diphosphate(ADP)and adenosine triphosphate(ATP);agarose gel electrophoresis was used to detect the expression of cyclooxygenase 2(COX-2)and heme oxygenase-1(HO-1)in HL-7702 cells.[Results]In Shuanghuanglian freeze-dried powder for injection,the content of baicalin was the highest,and the content of wogonin was the lowest.Compared with the control group,the expressions of AST and ALT in human hepatocytes(HL-7702)in high-dose baicalin group,forsythin group and wogonin group decreased(P<0.05,P<0.01),while the expression of ALT in chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)increased(P<0.05,P<0.01),and the expression of AST had no significant difference(P>0.05);the results in the low-dose group were similar to those in the high-dose group.Compared with the control group,ATP expression in chlorogenic acid group,chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)in the high-dose group decreased(P<0.05,P<0.01),and ADP expression was not significantly different(P>0.05);in the low-dose group,the expression of ATP and ADP increased in baicalin group(P<0.05),but decreased in wogonin group,baicalin+cefradine group(0.046 mg/mL)and wogonin group+cefradine group(0.046 mg/mL)(P<0.05,P<0.01).Compared with the control group,the expressions of COX-2 and HO-1 in HL-7702 cells in the cefradine group showed no significant difference(P>0.05).The expression of HO-1 and COX-2 in the different dose groups of Shuanghuanglian and the group combined with cefradine increased,and the difference was significant(P<0.05,P<0.01).[Conclusions]The components of Shuanghuanglian freeze-dried powder for injection had effects on hepatocytes,of which baicalin had a significant effect,and the effect of cefradine on hepatocytes was increased when used in combination with cefradine. | Junlong YU Dazhi XU Ruyan WEN Xiangling LI | 2022 | Medicinal Plant2022，13，6： | 0 |
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