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| 1 | Treg/Th17 cell balance and phytohaemagglutinin activation of T lymphocytes in peripheral blood of systemic sclerosis patients显示文摘AIM To investigate T-cell activation, the percentage of peripheral T regulatory cells(Tregs), Th17 cells and the circulating cytokine profile in systemic sclerosis(SSc).METHODS We enrolled a total of 24 SSc patients and 16 healthy controls in the study and divided the patients as having diffuse cutaneous SSc(dc SSc, n = 13) or limited cutaneous SSc(lc SSc, n = 11). We performed a further subdivision of the patients regarding the stage of the disease-early, intermediate or late. Peripheral venous blood samples were collected from all subjects. We performed flow cytometric analysis of the activationcapacity of T-lymphocytes upon stimulation with PHA-M and of the percentage of peripheral Tregs and Th17 cells in both patients and healthy controls. We used ELISA to quantitate serum levels of human interleukin(IL)-6, IL-10, tissue growth factor-β1(TGF-β1), and IL-17 A.RESULTS We identified a decreased percentage of CD3+CD69+ cells in PHA-stimulated samples from SSc patients in comparison with healthy controls(13.35% ± 2.90% vs 37.03% ± 2.33%, P < 0.001). However, we did not establish a correlation between the down-regulated CD3+CD69+ cells and the clinical subset, nor regarding the stage of the disease. The activated CD4+CD25+ peripheral lymphocytes were represented in decreased percentage in patients when compared to controls(6.30% ± 0.68% vs 9.36% ± 1.08%, P = 0.016). Regarding the forms of the disease, dc SSc patients demonstrated lower frequency of CD4+CD25+ T cells against healthy subjects(5.95% ± 0.89% vs 9.36% ± 1.08%, P = 0.025). With regard to Th17 cells, our patients demonstrated increased percentage in comparison with controls(18.13% ± 1.55% vs 13.73% ± 1.21%, P = 0.031). We detected up-regulated Th17 cells within the lc SSc subset against controls(20.46% ± 2.41% vs 13.73% ± 1.21%, P = 0.025), nevertheless no difference was found between dc SSc and lc SSc patients. Flow cytometric analysis revealed an increased percentage of CD4+CD25-Foxp3+ in dc SSc patients compared to controls(10.94% ± 1.65% vs 6.88% ± 0.91, P = 0.032). Regarding the peripheral cytokine profile, we detected raised levels of IL-6 [2.10(1.05-4.60) pg/m L vs 0.00 pg/m L, P < 0.001], TGF-β1(19.94 ± 3.35 ng/m L vs 10.03 ± 2.25 ng/m L, P = 0.02), IL-10(2.83 ± 0.44 pg/m L vs 0.68 ± 0.51 pg/m L, P = 0.008), and IL-17 A [6.30(2.50-15.60) pg/m L vs 0(0.00-0.05) pg/m L, P < 0.001] in patients when compared to healthy controls. Furthermore, we found increased circulating IL-10, TGF-β, IL-6 and IL-17 A in the lc SSc subset vs control subjects, as it follows: IL-10(3.32 ± 0.59 pg/m L vs 0.68 ± 0.51 pg/m L, P = 0.003), TGF-β1(22.82 ± 4.99 ng/m L vs 10.03 ± 2.25 ng/m L, P = 0.031), IL-6 [2.08(1.51-4.69) pg/m L vs 0.00 pg/m L, P < 0.001], and IL-17 A [14.50(8.55-41.65) pg/m L vs 0.00(0.00-0.05) pg/m L, P < 0.001]. Furthermore, circulating IL-17 A was higher in lc SSc as opposed to dc SSc subset(31.99 ± 13.29 pg/m L vs 7.14 ± 3.01 pg/m L, P = 0.008). Within the dc SSc subset, raised levels of IL-17 A and IL-6 were detected vs healthy controls: IL-17 A [2.60(0.45-9.80) pg/m L vs 0.00(0.00-0.05) pg/m L, P < 0.001], IL-6 [2.80(1.03-7.23) pg/m L vs 0.00 pg/m L, P < 0.001]. Regarding the stages of the disease, TGF-β1 serum levels were increased in early stage against late stage, independently from the SSc phenotype(30.03 ± 4.59 ng/m L vs 13.08 ± 4.50 ng/m L, P = 0.017).CONCLUSION It is likely that the altered percentage of Th17 and CD4+CD25-Fox P3+ cells along with the peripheral cytokine profile in patients with SSc may play a key role in the pathogenesis of the disease. | Ekaterina Krasimirova Tsvetelina Velikova Ekaterina Ivanova-Todorova Kalina Tumangelova-Yuzeir Desislava Kalinova Vladimira Boyadzhieva Nikolay Stoilov Tsvetelina Yoneva Rasho Rashkov Dobroslav Kyurkchiev | 2017 | World Journal of Experimental Medicine2017，7，3： | 10 |
| 2 | Self-healing effect of ceria electrodeposited thin films on stainless steel in aggressive 0.5 mol/L NaCl aqueous solution显示文摘The self-healing effect of electrochemically deposited CeO2-Ce2O3 films on stainless steel OC404(SS) in 0.5 mol/L NaCl solution was studied. It was established that the corrosion potential of the steel, after covering it with CeO2-Ce2O3 layer and thermal treatment(i.e. potential of the system CeO2-Ce2O3/SSt.t.), was shifted sharply to a considerably more positive value, while the corrosion current was reduced noticeably. The X-ray photoelectron spectroscopy(XPS), energy dispersive spectroscopy(EDS) and scanning electron microscopy(SEM) analyses on the observed scratched surface area of the system CeO2-Ce2O3/SSt.t., after exposure of investigated specimens to 0.5 mol/L NaCl corrosion media, showed partial accumulation of ceria, as well as remarkable increase in the concentrations of oxides of Al, Cr and Fe, on the mechanically revealed steel surface. On the basis of the obtained results one could conclude that the secondary passive oxide/hydroxide films, formed after a definite time interval of exposure to corrosion media, acted as barriers, hindering the corrosion processes in active zones. A hypothesis was put forward about the mechanism of self-repairing oxide layers on the steel surface and their corrosion protection effect respectively. | Desislava Guergova Emilia Stoyanova Dimitar Stoychev Ivalina Avramova Plamen Stefanov | 2015 | Journal of Rare Earths2015，33，11： | 1 |
| 3 | Groowth in transition:Reexamining the roles of factor inputs and geography显示文摘 | Desislava Rusinova | 2007 | Economic Systems2007，31，： | 1 |
| 4 | Is cultural distance a bane or a boon for cross-border acquisition performance?显示文摘 | Desislava Dikova Padma Rao Sahib | 2013 | Journal of World Business2013，，1： | 1 |
| 5 | Clinical analysis on neuroprotection of transient ischemic attacks显示文摘Transient ischemic attack(TIA) is an acute cerebrovascular incident,and is generally considered the best opportunity for early neuroprotective treatment against cerebral ischemia.This study retrospectively analyzed 80 patients with TIA(38 males and 42 females).Among 61 patients who received neuroprotective cerebrolysin treatment within 24 hours after TIA onset,13(21.31%) patients suffered subsequent strokes.Among 19 patients who received neuroprotective cerebrolysin treatment within 24-72 hours after TIA onset,seven(36.84%) developed cerebral infarction.There was a significant difference in the proportion of subsequent strokes between patients receiving cerebrolysin treatment within 24 hours and 24-72 hours after TIA onset(P = 0.438).These findings suggest that neuroprotective drugs administrated within 24 hours after TIA onset help reduce the incidence of subsequent strokes.The results demonstrate usefulness of the ABCD2 score at TIA patients in the determination of short-term and long-term cerebrovascular risk,including the frequency of subsequent ischemic cerebral infarctions up to 12 months. | Dimitar Maslarov Desislava Drenska | 2011 | Neural Regeneration Research2011，6，10： | 1 |
| 6 | Orthodontic- pros- thetic approach in the treatment of complex clinical cases 显示文摘 | Desislava Konstantinova Hristina Arnautska | 2014 | J IMAB2014，20，1： | 1 |
| 7 | Maternal serum retinol-bind- ing protein-4 at 11-13 weeks' gestation in normal and pathological pregnancies显示文摘 | Surabhi N George N Desislava M | 2013 | Metabol2013，62，6： | 1 |
| 8 | Performance of foreign subsidiaries: Does psychic distance matter?显示文摘 | Desislava Dikova | 2008 | International Business Review2008，，1： | 1 |
| 9 | The relationship between stock prices and ex-change rates: studied is multivariate Model显示文摘 | Desislava D | 2005 | Political Econ-omy2005，，14： | 1 |
| 10 | Orthodontic- prosthetic approach in the treatment of complex clinical cases 显示文摘 | Desislava Konstantinova Hristina Arnautska | 2014 | J IMAB2014，20，1： | 1 |
| 11 | The relationship between stock prices and exchange rates: studied is muhivariate Model 显示文摘 | Desislava D | 2005 | Political Economy2005，14，： | 1 |
| 12 | The relationship between stock prices and exchange rates: studied in multivariate model 显示文摘 | Desislava Dimitrova | 2005 | Political Economy2005，14，： | 1 |
| 13 | Growth in Transition: Reexamining the Roles of Factor Inputs and Geography 显示文摘 | Desislava R | 2007 | Economic systems2007，，31： | 1 |
| 14 | Information processing during sleep : the effect of olfactory stimuli on dream content and dream emotions 显示文摘 | Michael S Desislava A | 2009 | J Sleep Res2009，18，3： | 1 |
| 15 | Role of GM3 ganglioside in the pathology of some progressive human diseases and prognostic importance of serum anti-GM3 antibodies显示文摘Glycosphingolipids(gangliosides)have been characterized as important biological molecules with a key role as regulators in many physiological processes on cellular,tissue,organ,and organism levels.The deviations in their normal amounts,production,and metabolism are very often related to the development of many multi-factor socially important diseases.GM3 ganglioside,as a small molecule,plays important roles in the cascade regulatory pathways in the pathology of many disorders like neurodegenerative diseases,autoimmune diseases,inflammation,diabetes,malignant transformation,and others.Ganglioside GM3 and its derivatives are membrane-bound glycosphingolipids composed of an oligosaccharide head structure containing one sialic acid residue.These molecules transduce signals involved in cell surface events,including the phosphorylation of transmembrane receptors.This ganglioside is the most widely distributed among tissues,and it serves as a precursor for most of the more complex ganglioside species.GM3 inhibits the function of fibroblast growth factor receptor,and cell growth is regulated by GM3-enriched microdomain.GM3 is thought to inhibit immunologic functions,such as the proliferation and production of cytokines by T cells.On the other hand,the anti-ganglioside antibodies(AGAs)are important in many acquired demyelinating immunemediated neuropathies,like Multiple sclerosis(MS),Guillain–Barrésyndrome(GBS)and its variation,Miller–Fisher syndrome(MFS)and could be suggested as important diagnostic and prognostic markers about the describe diseases and their etiology.We show that the complexes of anti-ganglioside antibodies to GM3(detected by ELISA)may be useful diagnostic and prognostic tool markers for autoimmune diseases,neurodegenerative disorders,malignancy,diabetes,and inflammation.Our pilot studies suggest increased serum IgG anti-GM3 antibodies titers in patients with secondary progressive MS(SPMS),throat cancer,elder people with diabetes(89–96 years),old Lewis rats(30–33 months),and in the serum of subjected on lead intoxication BALB/c mice treated by salinomycin.We observed no changes in the titers in healthy elder people(89–96 years),in 70-year-old woman on dialysis,in relapsing-remitting MS(RRMS)patients on long-term treatment with Glatiramer acetate,Laquinimod,and Interferons,as well as in 18–22 months old Wistar rats and subjected on lead intoxication BALB/c mice treated by monensin and dimercaptosuccinic acid(DMSA).Considerable decrease of serum GM3 in early MS correlate with early damage and severe destruction of the blood–brain barrier,which provides impetus to initiate early therapy. | VERA KOLYOVSKA SONYA IVANOVA DESISLAVA DRENSKA DIMITAR MASLAROV RENETA TOSHKOVA | 2021 | BIOCELL2021，45，6： | 0 |
| 16 | Pharmacologic vitreolysis:New strategy for treatment of anomalous vitreo-macular adhesion显示文摘Persistent anomalous vitreo-macular adhesion(VMA) is a well-known factor, associated with a variety of sight threatening diseases- including macular hole, vitreo-macular traction syndrome, cystoid and diabetic macular edema, exudative age- related macular degeneration, myopic traction maculopathy and others. With the advent of optical coherence tomography our understanding of these pathologies and the ability of their early diagnosis has gone much far in the past two decades. The release of macular traction has been of exclusive surgical capability. Notwithstanding good results, vitrectomy is hampered by the inability of complete vitreo-retinal separation(i.e., smooth, bare internal limiting membrane), compulsory postoperative positioning in macular hole cases, surgical complications, and high costs. With aim to offer less invasive and safe treatment modality for anomalous VMA, investigators have made enormous progress in the past decade. Leading among the studied nonsurgical measures is the intravitreal application of pharmacologic agents for the induction of vitreo-retinal separation and vitreous liquefaction, a method termed pharmacologic vitreolysis. Several vitreolytic agents have been studied to date, the most potent among them proved to be plasmin. Recently, ocriplasmin(formerly known as microplasmin)- a more stable than plasmin recombinant product, proved to be safe and efficient in releasing VMA in large studies, and consequently received FDA approval. It's role in clinical practice is now in the process of being determined. This paper aims to review and summarize the current knowledge and status of investigation on this new approach for the treatment of VMA. | Desislava N Koleva-Georgieva | 2015 | World Journal of Ophthalmology2015，5，3： | 0 |
| 17 | 形式和颜色之间的舞蹈 与蒙德里安共进早餐显示文摘'与蒙德里安共进早餐'是一个实验性项目,空间的形式、使用的线条和颜色都旨在能够对使用者产生愉悦身心的积极影响。整个项目的设计灵感来源于荷兰画家蒙德里安的作品,他采用水平和垂直两种线条,以此构成各种不同的正方形和长方形,简单而又复杂的图形以红、黄、蓝三原色或黑、白、灰来表示色彩的纯净。在他的笔下色彩充满了活力,运用黄金分割和等比数列将画面或图形按照一定的比例进行分割、排列,创造富有节奏与韵律的秩序与美感,蒙德里安用画作展示了他如何将世界、自然和人类视为一体,他的画作呈现出一种不同元素在统一整体内的完美和谐。 | 〔保加利亚〕Branimira Ivanova Desislava Ivanova 悠悠(编译) | 2019 | 室内设计与装修2019，0，6： | 0 |
| 18 | 走进艺术世界 印象派花园显示文摘'印象派花园'所想要呈现空间就如同它的名字一样,设计师希望能够将使用者带入当下的艺术世界。这个住宅项目的设计灵感来源于19世纪一些伟大的印象派画家,而它的名字则直接源自画家克劳德·莫奈的花园。花园本身就是一个拥有各种形状和颜色的私密场所,住宅同样也是一个非常私人的空间,人们可以在这里放松、冥想,是一个可以卸下防备、不被束缚、释放自我的安全港湾。 | 〔保加利亚〕Branimira Ivanova Desislava Ivanova 叮当(编译) | 2019 | 室内设计与装修2019，0，6： | 0 |
| 19 | Indoor Radon Concentrations of School Buildings in Varna District, Bulgaria显示文摘Radon is a radioactive gas that is naturally produced by the breakdown of uranium in soil,rock and water.Recent International Commission on Radiological Protection(ICRP)and World Health Organization(WHO)requirements include controlling indoor radon exposure and settings of a reference level for the annual average activity concentration of up to 300 Bq/m3 for dwellings and public buildings.Radon measurements in school buildings have long been carried out in many countries.Systematic measurements in schools in Bulgaria have started and are progressing.The study concerns the measurements of radon concentration(CRn)in all state school buildings in one of the Black Sea regions,Varna district,and the analysis of indoor variation.The survey of 1,185 premises of 107 schools was conducted from December 2019/January 2020 to March/May 2020 using a passive method.The AM(Arithmetic Mean)of CRn in the premises of the studied schools on the territory of Varna district is 130 Bq/m^(3),and the geometric mean is 97 Bq/m^(3).The number of premises exceeding the national reference level of 300 Bq/m^(3) is 72 located in 38 schools(about 36% of schools).A statistically significant difference(KW(Kruskal-Wallis),p<0.0001)was found between the radon data,grouped by municipalities.Furthermore,the radon data were classified into two groups based on municipality location:on the Black Sea coast and inland.Schools located near the sea have a higher value of CRn.In order to more accurately assess the exposure of pupils and teachers,the results are divided into four groups,depending on the type of use of the premises and the floor on which they are located.The highest CRn value was found in the study rooms used for specialized education such as physics,chemistry,music etc.(AM=155 Bq/m3),which can be explained by their smaller size and less frequent use.A statistically significant difference(KW,p<0.0001)was found,which means that the radon exposure in the examined rooms depends on the type of use.The key to reducing children’s exposure in schools is to measure CRn and then apply corrective action to the high levels of radon. | Desislava Djunakova Bistra Kunovska Jana Djounova Kremena Ivanova Zdenka Stojanovska | 2022 | Journal of Environmental Science and Engineering(B)2022，11，2： | 0 |
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