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| 1 | Porous Calcium Phosphate Ceramic Scaffolds for Tissue Engineering显示文摘This study examined the biological response of two porous calcium phosphate ceramics, hydroxyapatite (HA) and hydroxyapaptite/β-tricalcium phosphate (HA/β-TCP) scaffolds. Three different cell types, a human osteoblastic cell line (HOS), primary human osteoblasts (HOB) and human mesenchymal stem cells (MSCs), were used to examine biocompatibility and osteoinductive capacity. The experimental results showed both materials were highly biocompatible and proliferation was significantly greater on pure HA (P< 0.01), with a peak in proliferation at day 7. Protein levels were significantly higher (P<0.05) than the control Thermanox (TMX(tm)) for both test materials. Osteoinduction of MSCs was observed on both test materials, with cells seeded on HA/β-TCP showing greater alkaline phosphatase activity compared to HA alone, indicating an enhancement in osteoinductive property. Both materials show good potential for use as tissue engineered scaffolds. | L Di Silvio N Gurav J Merry R Sambrook | 2005 | Journal of Wuhan University of Technology(Materials Science)2005，20，B12： | 5 |
| 2 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 3 | Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice显示文摘 | Collins Meredith A Bednar Filip Zhang Yaqing Brisset Jean-Christophe Galbán Stefanie Galbán Craig J Rakshit Sabita Flannagan Karen S Adsay N Volkan di Magliano Marina Pasca | 2012 | EN2012，，： | 3 |
| 4 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 5 | Sofosbuvir with pegylated interferon alfa-2a and ribavirin for treatment-naive patients with hepatitis C genotype-1 infection (ATOMIC): an open-label, randomised, multicentre phase 2 trial显示文摘 | Kris V Kowdley Eric Lawitz Israel Crespo Tarek Hassanein Mitchell N Davis Michael DeMicco David E Bernstein Nezam Afdhal John M Vierling Stuart C Gordon Jane K Anderson Robert H Hyland Hadas Dvory-Sobol Di An Robert G Hindes Efsevia Albanis William T Symo | 2013 | The Lancet2013，，9883： | 3 |
| 6 | Is there a relationship between dopamine and rhegmatogenous retinal detachment?显示文摘Dopamine and its receptors have been widely studied in the neurological conditions and in the retina. In this study, we evaluated the possible role of dopamine in rhegmatogenous retinal detachment(RRD) by comparing the amount of 3,4-dihydroxyphenylacetic acid(DOPAC), a surrogate index of retinal dopamin levels, in the vitreous sample of patients affected by RRD with those affected by macular pucker and vitre ous hemorrhage. Our results showed that significantly higher levels of DOPAC were found in the vitreou sample of patients affected by RRD compared with those affected by vitreous hemorrhage and macula pucker(P = 0.002). Specifically, no trace of the substance was found in vitreous hemorrhage and macula pucker samples. A slightly significant positive correlation was found among DOPAC and post-operativ best corrected visual acuity(r = 0.470, P = 0.049). No correlation was found between DOPAC and the day elapsed between diagnosis and surgery(P = 0.317). For the first time our findings suggest that DOPAC i released in RRD, but not in other retinal diseases such as vitreous hemorrhage and macular pucker. More over, we showed a correlation between visual acuity outcome and the amount of DOPAC in the vitreous This might have a potential, although still unknown, implication in the pathogenesis of the disease and/o in the associated photoreceptors loss. This study was approved by the Ethics Committee of Rome Tor Ver gata University Hospital(R.S.92.10) on September 24, 2010. | Alessio Martucci Massimo Cesareo Maria Dolores Pinazo-Durán Michela Di Pierro Matteo Di Marino Carlo Nucci Massimiliano Coletta Raffaele Mancino | 2020 | Neural Regeneration Research2020，15，2： | 2 |
| 7 | 在肝的 G6PT-H6PDH-11HSD1 三个一组和它在新陈代谢的症候群的 pathomechanism 的含意显示文摘 The metabolic syndrome, one of the most common clinical conditions in recent times, represents a combination of cardiometabolic risk determinants, including central obesity, glucose intolerance, insulin resistance, dyslipidemia, non-alcoholic fatty liver disease and hypertension. Prevalence of the metabolic syndrome is rapidly increasing worldwide as a consequence of common overnutrition and consequent obesity. Although a unifying picture of the pathomechanism is still missing, the key role of the pre-receptor glucocorticoid activation has emerged recently. Local glucocorticoid activation is catalyzed by a triad composed of glucose-6-phosphate-transporter, hexose-6-phosphate dehydrogenase and 11β-hydroxysteroid dehydrogenase type 1 in the endoplasmic reticulum. The elements of this system can be found in various cell types, including adipocytes and hepatocytes. While the contribution of glucocorticoid activation in adipose tissue to the pathomechanism of the metabolic syndrome has been well established, the relative importance of the hepatic process is less understood. This review summarizes the available data on the role of the hepatic triad and its role in the metabolic syndrome, by confronting experimental findings with clinical observations. | Ibolya Czegle Miklós Csala József Mandl Angelo Benedetti István Karádi Gábor Bánhegyi | 2012 | World Journal of Hepatology2012，4，4： | 2 |
| 8 | 遗传性肥胖与心房颤动的关系显示文摘观察性研究已经确定了体质量指数(body mass index,BMI)与心房颤动之间的关联。然而,从观察性研究中推断因果关系会受到混杂因素、逆因果关系和偏倚的影响。该研究的主要目的是应用BMI的遗传学预测因子评估BMI与心房颤动之间的因果关系。方法:纳入1987-2002年期间在美国、冰岛和荷兰实施的7个前瞻性人群队列研究中基线无心房颤动的欧洲血统个体51 646人, | 刘莉 叶鹏 Chatterjee NA Giulianini F Geelhoed B Lunetta KL Misialek JR Niemeijer MN Rienstra M Rose LM Smith AV Arking DE Ellinor PT Heeringa J Lin H Lubitz SA Soliman EZ Verweij N Alonso A Benjamin EJ Gudnason V Stricker BH van der Harst P Chasman DI Albert CM | 2017 | 中华高血压杂志2017，25，2： | 2 |
| 9 | Genetic Variation in IL28B Is Associated With Chronic Hepatitis C and Treatment Failure: A Genome-Wide Association Study显示文摘 | Andri Rauch Zoltán Kutalik Patrick Descombes Tao Cai Julia Di Iulio Tobias Mueller Murielle Bochud Manuel Battegay Enos Bernasconi Jan Borovicka Sara Colombo Andreas Cerny Jean–Fran?ois Dufour Hansjakob Furrer Huldrych F. Günthard Markus Heim Bernard Hirs | 2010 | Gastroenterology2010，，4： | 2 |
| 10 | Virus-related glomerular diseases : histological and clinical aspects 显示文摘 | Di Belgiojoso GB Felrario F Landriani N | 2002 | J Nephrol2002，15，： | 1 |
| 11 | Total gastrectomy for gastric cancer:can the type of lymphadenectomy condition the long-term results? 显示文摘 | Di Martino N Izzo G Cosenza A | 2005 | Suppl Tumori2005，4，3： | 1 |
| 12 | Pathogenesis of pterygium role of cytokines,growth factors, and matrix metal oproteinases 显示文摘 | di Girolamo N Chui J Coroneo MT | 2004 | Prog Retin Eye Res2004，23，2： | 1 |
| 13 | In-hospital outcome of patients with acute coronary syndrome relationship with inflammation and remodeling markers 显示文摘 | Giansante C Fiotti N Di Chiara A | 2007 | J Cardiovasc Med2007，8，8： | 1 |
| 14 | Natural course of functional dyspepsia after Helicobacter pylori eradication:a seven-year survey显示文摘 | Di Mario F Stefani N BòN D | 2005 | Dig Dis Sci2005，50，12： | 1 |
| 15 | Heparin and low-dose aspirin restore placental human chorlonic gonadotrophin secretion abolished by antiphospholipid antibody-containing sere 显示文摘 | Di simone N Ferrazzai S Castellani R | 1997 | Hum Reprod1997，12，： | 1 |
| 16 | Volatile anesthetics augment expression of proinflammatory cytokines in rat alveolar macrophages during mechanical ventilation显示文摘 | Kotani N Takahashi S Sessler DI | 1999 | Anesthesiology1999，91，： | 1 |
| 17 | A Conserved Cysteine Residue in Yeast Uroporphyrinogen Decarboxylase is not Essential for Enzymatic Activity显示文摘 | DI FLUMERI C ACHESON N H KENG T | 1997 | Can J Microbiol1997，43，： | 1 |
| 18 | The shivering threshold during Spinal anesthesia is reduced in the elderly显示文摘 | Vassilief N Rosencher N Sessler DI | 1995 | Anesthesology1995，83，6： | 1 |
| 19 | Enzyme activity and stability control by amphiphilic self - organizing systems in aqueous solutions 显示文摘 | SAVELLI G SPRETI N DI PROFIO P | 2000 | Current Opinion in Colloid & Interface Science2000，5，： | 1 |
| 20 | Frequency and determinants for hemorrhagic transformation of cerebral infarction显示文摘 | Terruso V ErAmelio M Di Benedetto N | 2009 | Neuroepidemiology2009，33，3： | 1 |
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