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| 1 | Parkinson’s disease-associated VPS35 mutant reduces mitochondrial membrane potential and impairs PINK1/Parkinmediated mitophagy显示文摘Background:Mitochondrial dysfunction plays a prominent role in the pathogenesis of Parkinson’s disease(PD),and several genes linked to familial PD,including PINK1(encoding PTEN-induced putative kinase 1[PINK1])and PARK2(encoding the E3 ubiquitin ligase Parkin),are directly involved in processes such as mitophagy that maintain mitochondrial health.The dominant p.D620N variant of vacuolar protein sorting 35 ortholog(VPS35)gene is also associated with familial PD but has not been functionally connected to PINK1 and PARK2.Methods:To better mimic and study the patient situation,we used CRISPR-Cas9 to generate heterozygous human SH-SY5Y cells carrying the PD-associated D620N variant of VPS35.These cells were treated with a protonophore carbonyl cyanide m-chlorophenylhydrazone(CCCP)to induce the PINK1/Parkin-mediated mitophagy,which was assessed using biochemical and microscopy approaches.Results:Mitochondria in the VPS35-D620N cells exhibited reduced mitochondrial membrane potential and appeared to already be damaged at steady state.As a result,the mitochondria of these cells were desensitized to the CCCPinduced collapse in mitochondrial potential,as they displayed altered fragmentation and were unable to accumulate PINK1 at their surface upon this insult.Consequently,Parkin recruitment to the cell surface was inhibited and initiation of the PINK1/Parkin-dependent mitophagy was impaired.Conclusion:Our findings extend the pool of evidence that the p.D620N mutation of VPS35 causes mitochondrial dysfunction and suggest a converging pathogenic mechanism among VPS35,PINK1 and Parkin in PD. | Kai Yu Ma Michiel R.Fokkens Fulvio Reggiori Muriel Mari Dineke S.Verbeek | 2021 | Translational Neurodegeneration2021，10，2： | 3 |
| 2 | 中国文化遗产的英文解说:以北京故宫博物院为例显示文摘通过社交媒体的传播,游客对目的地形象塑造发挥着越来越重要的作用。积极、难忘的旅游体验常常成为游客网络交谈的主要内容。尤其是遗产旅游,满足游客互动及共创体验的遗产地解说可以建立游客与遗产地之间的情感联系,提升游客体验效果。然而,目前故宫博物院中的现场标识及语音导览中的英文解说以陈述景点概况为主,很难使游客身临其境地感受历朝历代的生活场景。如今,各式移动终端技术为游客提供了更多共创及浸入式旅游体验模式,而对于故宫博物院来说,故事化、主题化、含音乐背景的可下载式电子导览即可为游客提供更加个性化、现代化的英文解说,从而为游客创造更难忘的旅游体验。 | Dineke Koerts 撒露莎(译) | 2017 | 中南民族大学学报(人文社会科学版)2017，37，2： | 2 |
| 3 | Large thrombus at the site of primary sutured atrial septal defect associated with pulmonary emboli-sm and treatment by thrombolysis 显示文摘 | Dinekal MH Davutoglu V Soydinc S et el | 2003 | Echocardiography2003，20，6： | 1 |
| 4 | The Development and Validation of A Framework for Teaching Compe- tencies in Higher Education显示文摘 | DINEKE E H DIANAH J M INEKE H P | 2004 | Higher Education2004，48，2： | 1 |
| 5 | Associative stigma in family members of psychotic patients in Flanders:An exploratory study显示文摘AIM: To assess presence and severity of associative stigma in family members of psychotic patients and factors for higher associative stigma.METHODS: Standardized semi-structured interview of 150 family members of psychotic patients receiving full time treatment. This study on associative stigma in family members of psychotic patients was part of a larger research program on the burden of the family, using 'Interview for the Burden of the Family' and the chapters stigma, treatment and attribution from the 'Family interview Schedule'. The respondents were relatives, one per patient, either partner or parent. The patients had been diagnosed with schizophrenia or schizo-affective disorder. All contacts with patients and relatives were in Dutch. Relatives were deemed suitable to participate in this research if they saw the patient at least once a week. Recruitment took place in a standardized way: after obtaining the patient's consent, the relatives were approached to participate. The results were analyzed using SPSS Version 18.0. RESULTS: The prevalence of associative stigma in this sample is 86%. Feelings of depression in the majority of family members are prominent. Twenty-one point three percent experienced guilt more or less frequent, while shame was less pronounced. Also, 18.6% of allrespondents indicated that they tried to hide the illness of their family member for others regularly or more. Three six point seven percent really kept secret about it in certain circumstances and 29.3% made efforts to explain what the situation or psychiatric condition of their family member really is like. Factors with marked significance towards higher associative stigma are a worsened relationship between the patient and the family member, conduct problems to family members, the patients' residence in a residential care setting, and hereditary attributional factors like genetic hereditability and character. The level of associative stigma has significantly been predicted by the burden of aggressive disruptions to family housemates of the psychotic patient.CONCLUSION: Family members of psychotic patients in Flanders experience higher associative stigma compared to previous international research. Disruptive behavior by the patient towards in-housing family members is the most accurate predictor of higher associative stigma. | Kirsten Catthoor Didier Schrijvers Joost Hutsebaut Dineke Feenstra Philippe Persoons Marc De Hert Jozef Peuskens Bernard Sabbe | 2015 | World Journal of Psychiatry2015，5，1： | 1 |
| 6 | The development and validation of a framework for teaching competencies in higher education显示文摘 | Dineke E H | 2004 | Higher Education2004，48，03： | 1 |
| 7 | Effects of exercise training on cardiac performance, exercise capacity and quality of life in patients with heart failure: A meta- analysis显示文摘 | Benno AF Rosalie JH Dineke WK | 2006 | Eur J Heart Fail2006，8，： | 1 |
| 8 | The development and validation of a frame work for teaching competeneies in higher edueation显示文摘 | DINEKE E H Tigelaar DIANA H J M Dolmans INEKE H A P Wolfhagen | 2004 | Higher Education2004，48，2： | 1 |
| 9 | The development and validation of a framework for teaching competencies in higher education显示文摘 | Dineke E.H. Tigelaar Diana H.J.M. Dolmans Ineke H.A.P. Wolfhagen Cees P.M. van der Vleuten | 2004 | Higher Education2004，，2： | 1 |
| 10 | The development and validation of a frame ework for teaching competencies in higher education显示文摘 | Dineke EH Diana HJM Ineke HAP | 2004 | Higher Education2004，48，2： | 1 |
| 11 | The Development and Vail for Teaching Competencies in Higher Education,2004(48) dation of a Framework Education 显示文摘 | Dineke E | 2004 | Higher2004，，： | 1 |
| 12 | The Development and Vali~ dation of a Framework for Teaching Competencies in Higher Education显示文摘 | Dineke E H Tigelaar | 2004 | Higher Education2004，，48： | 1 |
| 13 | The development and validation of a framework for teaching competencies in higher education显示文摘 | Dineke E.H Diana H.J.M Ineke H.A.P | | 0，，02： | 1 |
| 14 | The development and validation of a framework for teaching competencies in higher education显示文摘 | Dineke E.H. Tigelaar Diana H.J.M. Dolmans Ineke H.A.P. Wolfhagen Cees P.M. van der Vleuten | 2004 | Higher Education2004，，2： | 1 |
| 15 | The development and valida显示文摘 | Tigelaar Diana H J M Dolmans Inekeh A P Wolfhagen Cees P M Vander V | 2004 | Higher Education2004，，3： | 1 |
| 16 | Severe infantile Bordetella pertussis pneumonia in monozygotic twins with a congenital C3 deficiency显示文摘 | Roel A. J. Kurvers Dineke Westra Arno F. Heijst Twiggy L. M. Walk Adilia Warris Nicole C. A. J. Kar | 2014 | European Journal of Pediatrics2014，，12： | 1 |
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