| 4 | A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain显示文摘Nucleic acid detection plays a key role in diverse diagnosis and disease control.Currently available nucleic acid detection techniques are challenged by trade-offs among speed,simplicity,precision and cost.Here,we described a novel method,designated SENSOR(Sulfur DNA mediated nucleic acid sensing platform),for rapid nucleic acid detection.SENSOR was developed from phosphorothioate(PT)-DNA and sulfur binding domain(SBD)which specifically binds double-stranded PT-modified DNA.SENSOR utilizes PT-DNA oligo and SBD as targeting module,which is linked with split luciferase reporter to generate luminescence signal within 10 min.We tested detection on synthesized nucleic acid and COVID-19 pseudovirus,achieving attomolar sensitivity combined with an amplification procedure.Single nucleotide polymorphisms(SNP)could also be discriminated.Indicating SENSOR a new promising nucleic acid detection technique. | Yuting Shuai Yi Ju Yuanhang Li Dini Ma Lan Jiang Jingyu Zhang Gao-Yi Tan Xueting Liu Shenlin Wang Lixin Zhang Guang Liu | 2023 | Synthetic and Systems Biotechnology2023，8，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员