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您的检索式：作者名="Donald Harn"
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| 1 | Real-time PCR diagnosis of Schistosoma japonicum in low transmission areas of China显示文摘Background:Schistosomiasis in the People’s Republic of China(PRC)can be traced back to antiquity.In the past 60 years,the Chinese government has made great efforts to control this persistent disease with elimination slated by 2020 through the implementation of a comprehensive control strategy.This strategy aims to reduce the role of bovines and humans as sources of infection as a pre-requisite for elimination through transmission interruption.The goal of elimination will be achievable only by the implementation of a sustainable surveillance and control system,with sensitive diagnosis a key feature so that the true disease burden is not underestimated.Currently used diagnostics lack the necessary sensitivity to accurately determine the prevalence of Schistosoma japonicum infection in areas with low infection intensities.It is of critical importance to find and treat people and to identify animals with low-level infections if the National Control Programme for China is to achieve schistosomiasis elimination.Methods:We evaluated a real-time polymerase chain reaction(qPCR)assay using 633 human stool samples collected from five villages in Hunan,Anhui,Hubei,and Jiangxi provinces,and 182 bovine(70 cattle and 112 buffalo)stool samples obtained from four villages in Hunan,Anhui,and Jiangxi provinces in the PRC.All stool samples were subjected to the miracidium hatching test(MHT,a diagnostic procedure used in the National Schistosomiasis Control Programme)and the qPCR assay.Samples positive by MHT were subjected to either the Kato-Katz technique for humans,or the formalin-ethyl acetate sedimentation-digestion(FEA-SD)procedure for bovines,to determine infection intensities.Results:The qPCR assay exhibited a high level of sensitivity in the detection of S.japonicum infections.With both the human and bovine samples,a significantly higher prevalence was determined using the qPCR assay(11.06%humans,24.73%bovines)than with the MHT(0.93%humans,7.69%bovines).The animal contamination index(calculated using data obtained with the qPCR technique)for all positive bovines was 27618000 eggs per day,indicating a considerable amount of environmental egg contamination that would be underestimated using less sensitive diagnostic procedures.Conclusions:The qPCR assay we have evaluated will be applicable as a future field diagnostic and surveillance tool in low-transmission zones where schistosomiasis elimination is targeted and for monitoring post-intervention areas to verify that elimination has been maintained. | Pei He Catherine A.Gordon Gail M.Williams Yuesheng Li Yuanyuan Wang Junjian Hu Darren J.Gray Allen G.Ross Donald Harn Donald P.McManus | 2018 | Infectious Diseases of Poverty2018，7，1： | 7 |
| 2 | 抗日本血吸虫卵单克隆抗体的制备和免疫化学的鉴定显示文摘用可溶性日本血吸虫卵抗原(SEA)免疫小鼠制备单克隆抗体,获得5株特异的抗日本血吸虫卵单抗。免疫球蛋白亚类鉴定均为IgG_1。免疫印迹法结果表明其所识别的日本血吸虫卵抗原的分子量分别为>200kDa、116kDa和22kDa。经两维免疫印迹法(2-Dimensional Western Blot),显示被单抗IE1识别的22kDa SEA等电点pI4.6—6之间至少有3个以上同晶型体(isomorphs)。放射免疫沉淀试验表明IE1可识别30kDa抗原。 | 许阿莲 周晓音 王炳夫 Donald A Harn | 1992 | 中国寄生虫学与寄生虫病杂志1992，10，1： | 2 |
| 3 | The chronic enteropathogenic disease schistosomiasis显示文摘 | David U. Olveda Remigio M. Olveda Donald P. McManus Pengfei Cai Thao N.P. Chau Alfred K. Lam Yuesheng Li Donald A. Harn Marilyn L. Vinluan Allen G.P. Ross | 2014 | International Journal of Infectious Diseases2014，，： | 1 |
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