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| 1 | Mesenchymal stromal cell-based therapy: Regulatory and translational aspects in gastroenterology显示文摘The past decade has witnessed an outstanding scientific production focused towards the possible clinical applications of mesenchymal stromal cells(MSCs) in autoimmune and chronic inflammatory diseases. This raised the need of novel standards to adequately address quality, efficacy and safety issues of this advanced therapy. The development of a streamlined regulation is currently hampered by the complexity of analyzing dynamic biological entities rather than chemicals. Although numerous pieces of evidence show efficacy in reducing intestinal inflammation, some inconsistencies between the mechanisms of action of rodent vs human MSCs suggest caution before assigning translational value to preclinical studies. Preliminary evidence from clinical trials showed efficacy of MSCs in the treatment of fistulizing Crohn's disease(CD), and preparations of heterologous MSCs for CD treatment are currently tested in ongoing clinical trials. However, safety issues, especially in longterm treatment, still require solid clinical data. In this regard, standardized guidelines for appropriate dosing and methods of infusion could enhance the likelihood to predict more accurately the number of responders and the duration of remission periods. In addition, elucidating MSC mechanisms of action could lead to novel and more reliable formulations such as those derived from the MSCs themselves(e.g., supernatants). | Giovanni Dothel Emanuel Raschi Roberto Rimondini Fabrizio De Ponti | 2016 | World Journal of Gastroenterology2016，22，41： | 3 |
| 2 | Animal models of chemically induced intestinal inflammation:predictivity and ethical issues显示文摘 | Dothel G Vasina V Barbara G | 2013 | Pharmacology and Therapeutics2013，139，1： | 1 |
| 3 | Animal models ofchemically induced intestinal inflammation : Predictivity andethical issues显示文摘 | Dothel G Vasina V Barbara G | 2013 | Pharmacology & Therapeutics2013，139，： | 1 |
| 4 | Animal models of chemically induced intestinal inflammation:Predictivity and ethical issues显示文摘 | DOTHEL G VASINA V BARBARA G | 2013 | Pharmacology & Therapeutics2013，139，： | 1 |
| 5 | Nerve Fiber Outgrowth Is Increased in the Intestinal Mucosa of Patients With Irritable Bowel Syndrome显示文摘 | Giovanni Dothel Maria Raffaella Barbaro Hélène Boudin Valentina Vasina Cesare Cremon Luciana Gargano Lara Bellacosa Roberto De Giorgio Catherine Le Berre-Scoul Philippe Aubert Michel Neunlist Fabrizio De Ponti Vincenzo Stanghellini Giovanni Barbara | 2015 | Gastroenterology2015，，5： | 1 |
| 6 | Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia显示文摘BACKGROUND Mesenchymal stromal cell(MSC)-based therapy is currently under study to treat inflammatory bowel diseases.MSC bioactive products could represent a valid alternative to overcome issues associated with systemic whole-cell therapies.However,MSC anti-inflammatory mechanisms differ between rodents and humans,impairing the reliability of preclinical models.AIM To evaluate the effect of conditioned medium(CM)derived from porcine vascular wall MSCs(pVW-MSCs)on survival and differentiation of porcine and guinea pig enteric ganglia exposed to lipopolysaccharide(LPS).METHODS Primary cultures of enteric ganglia were obtained by mechanic and enzymatic digestion of ileum resections from guinea pigs(Cavia porcellus)(GPEG)and pigs(Suus scrofa)(PEG).pVW-MSCs were derived by enzymatic digestion from vascular wall resections of porcine aorta and tested by immunoflowcytometry for MSC immune profile.Enteric ganglia were treated with increasing concentrations of LPS,CM derived by pVW-MSCs or a combination of CM and LPS 1μg/mL.Cell count and morphometric analysis of HuD positive neurons and glial fibrillary acidic protein positive glial cells were performed by immunofluorecent staining of cultured ganglia.RESULTS PEG showed a higher number of neurons compared to GPEG.Overall,CM exerted a protective role on LPS-treated enteric ganglia.CM in combination with LPS increased the number of glial cells per ganglion in both cultures evoking glial cells differentiation in porcine cultures.CONCLUSION These findings suggest an immunomodulating activity of pVW-MSCs mediators on the enteric nervous system in inflammatory conditions. | Giovanni Dothel Chiara Bernardini Augusta Zannoni Maria Rosaria Spirito Roberta Salaroli Maria Laura Bacci Monica Forni Fabrizio De Ponti | 2019 | World Journal of Gastroenterology2019，25，33： | 0 |
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