| 1 | Activation of Toll-like receptors signaling in non-small cell lung cancer cell line induced by tumor-associated macrophages显示文摘Background:Inflammation is often linked with the progress and poor outcome of lung cancer.The understanding of the relationship between tumor-associated macrophages(TAMs) and lung cancer cells involves in the underlying mechanism of inflammatory cytokine production.Toll-like receptors(TLRs) are engaged in promoting the production of pro-inflammatory cytokines and play an important role in tumor immunology.Methods:To investigate the mechanisms by which TAMs influence the production of pro-inflammatory cytokines in lung cancer cells,we established an in vitro coculture system using TAMs and human nonsmall cell lung cancer(NSCLC) cell line SPC-A1.Levels of interleukin(IL)-1β,IL-6 and IL-8 in SPC-A1 were evaluated by RT-PCR and cytometric bead array assay after being cocultured with TAMs.Expression changes of TLRs and TLRs signaling pathway proteins in SPC-A1 were further confirmed by RT-PCR and western blot.The level changes of IL-1β,IL-6 and IL-8 in SPC-A1 were also detected after the stimulation of TLRs agonists.Results:We found that the phenotype markers of TAMs were highly expressed after stimulating human monocyte cell line THP-1 by phorbol-12-myristate-13-acetate(PMA).Higher mRNA and supernate secretion levels of IL-1β,IL-6 and IL-8 were detected in SPC-A1 after being cocultured with TAMs.We also found that TLR1,TLR6 and TLR7 were up-regulated in SPC-A1 in the coculture system with TAMs.Meanwhile,TLRs signaling pathway proteins were also significantly activated.Moreover,pre-treatment with agonist ligands for TLR1,TLR6 and TLR7 could dramatically promote inductions of IL-1β,IL-6 and IL-8.Conclusions:These findings demonstrated that TAMs may enhance IL-1β,IL-6 and IL-8 expressions via TLRs signaling pathway.We conclude that TAMs contribute to maintain the inflammation microenvironment and ultimately promote the development and progression of lung cancer. | Xing Ke Meng Wu Jianfang Lou Shuping Zhang Peijun Huang Ruihong Sun Lei Huang Erfu Xie Fang Wang Bing Gu | 2015 | Chinese Journal of Cancer Research2015，27，2： | 6 |
| 2 | Calibration and verification of streaked optical pyrometer system used for laser-induced shock experiments显示文摘Although the streaked optical pyrometer(SOP)system has been widely adopted in shock temperature measurements,its reliability has always been of concern.Here,two calibrated Planckian radiators with different color temperatures were used to calibrate and verify the SOP system by comparing the two calibration standards using both multi-channel and single-channel methods.A high-color-temperature standard lamp and a multi-channel filter were specifically designed for the measurement system.To verify the reliability of the SOP system,the relative deviation between the measured data and the standard value of less than 5%was calibrated out,which demonstrates the reliability of the SOP system.Furthermore,a method to analyze the uncertainty and sensitivity of the SOP system is proposed.A series of laserinduced shock experiments were conducted at the‘Shenguang-Ⅱ’laser facility to verify the reliability of the SOP system for temperature measurements at tens of thousands of kelvin.The measured temperature of the quartz in our experiments agreed fairly well with previous works,which serves as evidence for the reliability of the SOP system. | Zhiyu He Guo Jia Fan Zhang Xiuguang Huang Zhiheng Fang Jiaqing Dong Hua Shu Junjian Ye Zhiyong Xie Yuchun Tu Qili Zhang Erfu Guo Wenbing Pei Sizu Fu | 2019 | High Power Laser Science and Engineering2019，7，3： | 0 |
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