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| 1 | Activation of Toll-like receptors signaling in non-small cell lung cancer cell line induced by tumor-associated macrophages显示文摘Background:Inflammation is often linked with the progress and poor outcome of lung cancer.The understanding of the relationship between tumor-associated macrophages(TAMs) and lung cancer cells involves in the underlying mechanism of inflammatory cytokine production.Toll-like receptors(TLRs) are engaged in promoting the production of pro-inflammatory cytokines and play an important role in tumor immunology.Methods:To investigate the mechanisms by which TAMs influence the production of pro-inflammatory cytokines in lung cancer cells,we established an in vitro coculture system using TAMs and human nonsmall cell lung cancer(NSCLC) cell line SPC-A1.Levels of interleukin(IL)-1β,IL-6 and IL-8 in SPC-A1 were evaluated by RT-PCR and cytometric bead array assay after being cocultured with TAMs.Expression changes of TLRs and TLRs signaling pathway proteins in SPC-A1 were further confirmed by RT-PCR and western blot.The level changes of IL-1β,IL-6 and IL-8 in SPC-A1 were also detected after the stimulation of TLRs agonists.Results:We found that the phenotype markers of TAMs were highly expressed after stimulating human monocyte cell line THP-1 by phorbol-12-myristate-13-acetate(PMA).Higher mRNA and supernate secretion levels of IL-1β,IL-6 and IL-8 were detected in SPC-A1 after being cocultured with TAMs.We also found that TLR1,TLR6 and TLR7 were up-regulated in SPC-A1 in the coculture system with TAMs.Meanwhile,TLRs signaling pathway proteins were also significantly activated.Moreover,pre-treatment with agonist ligands for TLR1,TLR6 and TLR7 could dramatically promote inductions of IL-1β,IL-6 and IL-8.Conclusions:These findings demonstrated that TAMs may enhance IL-1β,IL-6 and IL-8 expressions via TLRs signaling pathway.We conclude that TAMs contribute to maintain the inflammation microenvironment and ultimately promote the development and progression of lung cancer. | Xing Ke Meng Wu Jianfang Lou Shuping Zhang Peijun Huang Ruihong Sun Lei Huang Erfu Xie Fang Wang Bing Gu | 2015 | Chinese Journal of Cancer Research2015，27，2： | 6 |
| 2 | 食品废物两相高温半干法厌氧发酵生产沼气初步研究显示文摘为了提高两相厌氧发酵工艺的生产性能,该文研究了一种新的两相半干法厌氧发酵沼气生产系统,即在水解相中批式发酵,每次从工作容积为4L的产烷相中取500mL接种液,与食品废物混合后发酵1~2d,再全部输入产烷相继续生产沼气,如此循环。实验运行温度为50℃,食品废物总固体TS和挥发性固体VS分别为19.3%和16.1%,产烷相先在4gVS·d-1的进料率下单相运行至稳定,稳定期日产气量为(4.09±0.03)L(甲烷占69.0%),pH为7.50±0.02,TS和VS分别为(1.05±0.02)%和(0.64±0.02)%。稳定的产烷相与水解相联合后,水解相在60~700g的进料量下都能正常产气,但氢气含量较低。产烷相在进料量600g时产气量最高,为29.1L/d,随后受到抑制,受抑制前的甲烷含量为72.4%~74.0%,TS和VS含量也在该进料量后达到最高峰,分别为3.50%和2.32%。研究结果表明新的两相半干法厌氧发酵沼气生产系统有效提高了生产性能,在今后的研究中,需要降低从产烷相输入水解相的接种液量,以更好抑制水解相产烷微生物,提高产氢性能,还可使产烷相更加稳定,承受更高的进料量。 | 泮进明 张瑜 Zhang Ruihong 朱保宁 Sun Huawei 应义斌 | 2008 | 农业工程学报2008，24，7： | 4 |
| 3 | Cosmological evolution of quintessence with a sign-changing interaction in dark sector显示文摘Dark energy and dark matter are only indirectly measured though their gravitational effects.It is possibie that there is some direct,non-gravitational interaction between DE and DM,which can be used to solve(or,at least alleviate)several important theoretical problems.In the present work,by analysing the cosmological dynamical system with a dark-sector interaction which changes its sign during the cosmological evolution,we find a scaling attractor to help to alleviate the cosmic-coincidence problem.This result shows that this interaction can bring new features to the cosmology. | ZHANG Ming SUN ChengYi YANG ZhanYing YUE RuiHong | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，9： | 3 |
| 4 | Photocatalytic Oxidation Kinetics of Thiophene with Nano-F^-/Fe^(3+)/TiO_2显示文摘Photocatalytic oxidation kinetics of thiophene in n-octane/water extraction system was studied with fluorine and ferric ion codoped nano-TiO2(nano-F-/Fe3+/TiO2) powders used as the photocatalyst.Effects of initial concentration of thiophene and additional dosage of F-/Fe3+/TiO2 on the reaction rate constant and half-life were investigated.The results showed that the appropriately added dosage of F-/Fe3+/TiO2 was 0.1 g in the 100-mL reaction system and the photooxidative kinetics of thiophene in the presence of F-/Fe3+/TiO2 catalyst was of first-order with a rate constant of 0.6508 h-1 and a half-life of 1.0651 h.The desulfurization rate of thiophene was 98.1%in 5 h and the sulfur content could be reduced from 800 ppm to 15 ppm.The reaction rate constant increased with a decreasing initial concentration of thiophene. | Li Fatang~1 Sun Zhimin~1 Liu Ying~1 Zhao Dishun~2 Liu Ruihong~1 (1.College of Science,Hebei University of Science and Technology,Shijiazhuang 050018 2.College of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology) | 2009 | China Petroleum Processing & Petrochemical Technology2009，11，4： | 2 |
| 5 | Involvement of mitogen-activated protein kinase pathways in N-methyl-D-aspartate-induced excitotoxicity显示文摘Previous studies have shown that mitogen-activated protein kinase(MAPK) signaling pathways are involved in N-methyl-D-aspartate(NMDA)-mediated excitotoxicity.However,a systematic observation or analysis of the role of these various MAPK pathways in excitotoxicity processes does not exist.The present study further evaluated the role and contribution of three MAPK pathways extracellular signal-regulated kinase,c-Jun N-terminal kinase,and p38 MAPK in an NMDA-mediated excitotoxicity model using MAPK-specific inhibitor.Results demonstrated that c-Jun N-terminal kinase inhibitor SP600125 and/or p38 MAPK inhibitor SB203580 inhibited NMDA-induced reduction in cell viability,as well as reduced NMDA-induced lactate dehydrogenase leakage and reactive oxygen species production.However,PD98059,an inhibitor of extracellular signal-regulated kinase,did not influence this model.Results demonstrated an involvement of c-Jun N-terminal kinase and p38 MAPK,but not extracellular signal-regulated kinase,in NMDA-mediated excitotoxicity in cortical neurons. | Xiaorong Yang Ping Sun Huaping Qin Rui Wang Ye Wang Ruihong Shi Xin Zhao Ce Zhang | 2011 | Neural Regeneration Research2011，6，4： | 1 |
| 6 | An ultrapotent pan-β-coronavirus lineage B(β-CoV-B)neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope显示文摘New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design.Here,we identified a receptorbinding domain(RBD)-binding antibody,XG014,which potently neutralizesβ-coronavirus lineage B(β-CoV-B),including SARS-CoV-2,its circulating variants,SARSCoV and bat SARSr-CoV WIV1.Interestingly,antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibodydependent SARS-CoV-2 spike(S)protein-mediated cellcell fusion,suggesting a unique mode of recognition by XG014.Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional“down”conformation,while its family member XG005 directly competes with ACE2 binding and position the RBD“up”.Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo.Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines againstβ-CoV-B and newly emerging SARS-CoV-2 variants of concern. | Zezhong Liu Wei Xu Zhenguo Chen Wangjun Fu Wuqiang Zhan Yidan Gao Jie Zhou Yunjiao Zhou Jianbo Wu Qian Wang Xiang Zhang Aihua Hao Wei Wu Qianqian Zhang Yaming Li Kaiyue Fan Ruihong Chen Qiaochu Jiang Christian TMayer Till Schoofs Youhua Xie Shibo Jiang Yumei Wen Zhenghong Yuan Kang Wang Lu Lu Lei Sun Qiao Wang | 2022 | Protein & Cell2022，13，9： | 1 |
| 7 | Photocatalytic Oxidative Desulfurization of Gasoline by TiO_2 in[BMIm]Cu_2Cl_3 Ionic Liquid显示文摘Photocatalytic oxidative desulfurization of gasoline in[BMIm]Cu_2Cl_3 ionic liquid was studied.A500-W high-pressure mercury lamp was used as the light source for irradiation,nano-TiO2 was used as thephotocatalyst and air was introduced by a gas pump to supply O_2 as the oxidant.Influence of the ratio ofV(ionic liquid) to V(oil) and the TiO_2 addition on the desulfurization rate of gasoline was investigated.Anoxidative kinetics equation was founded.The results showed that the [BMIm]Cu2CIs ionic liquid was aneffective extractant for the desulfurization of gasoline.The appropriate TiO_2 addition was 0.05 g in 50 mLof reaction mixture.The yield of desulfurized gasoline could reach 98.2% after being subjected to reactionfor 2 h under the conditions of adopting a ratio of V(ionic liquid):V(oil)=1:4,an air flow of 100 mL/min anda TiO_2 addition dosage of 0.05 g.The kinetics reaction for photo-oxidation of gasoline was a first-orderreaction with an apparent rate constant of 1.9664 h^(-1)and a half-time of 0.3525 h. | Li Fatang Liu Ruihong Sun Zhimin | 2008 | China Petroleum Processing & Petrochemical Technology2008，10，4： | 1 |
| 8 | Microstructure and strengthening mechanisms of molybdenum alloy wires doped with lanthanum oxide particles显示文摘 | Guo-jun Zhang Gang Liu Yuan-jun Sun Feng Jiang Lin Wang Ruihong Wang Jun Sun | 2008 | International Journal of Refractory Metals and Hard Materials2008，，1： | 1 |
| 9 | Interobserver Agreement for Classifying Infections in Patients with Decompensated Cirrhosis Based on Centers for Disease Control and Prevention Criteria显示文摘Background:Bacterial infections are common in patients with decompensated cirrhosis,largely owing to bacterial translocation and cirrhosis-associated immune dysfunction.This study aims to determine the reliability for classifying infections in patients with decompensated cirrhosis based on the Centers for Disease Control and Prevention(CDC)criteria.Methods:The patients with decompensated cirrhosis with suspicious infection in a registered prospective cohort of cirrhosis from May 1,2014 to February 25,2015 in the ward of First Affiliated Hospital of Zhejiang University were retrospectively identified.Agreement assessment was conducted focusing on site of infection,the possibility of infection,and pathogens of infection on both system level and specific diagnosis level.A subgroup analysis was performed based on with/without acute-on-chronic liver failure(ACLF).Results:A total of 402 infectious episodes among 351 patients were enrolled for consistency analysis.The overall agreement for site of infection was 94%(378/402)(k=0.90,95%CI 0.86–0.94)on system level and 86%(346/402)(k=0.84,95%CI 0.80–0.88)on specific diagnosis level.On possibility of infection,the overall agreement was 81%(306/378)(weighted k=0.71,95%CI 0.65–0.77),with 84%(224/267)(weighted k=0.75,95%CI 0.63–0.87)in patients with ACLF and 80%(70/88)(weighted k=0.68,95%CI 0.60–0.76)in patients without ACLF,respectively.On pathogens of infection,the overall agreement was 72%(60/83)(k=0.70,95%CI 0.60–0.80)among most frequent infections.Conclusion:The agreement for classifying infections in patients with decompensated cirrhosis based on CDC criteria is acceptable overall,suggesting that it can be a useful tool for clinical management in patients with decompensated cirrhosis with suspicious infections. | Haotang Ren Junjie Yao Ruihong Zhao Kai Gong Shanshan Sun Xia Yu Wei Shen Jinnan Duan Jifang Sheng Yu Shi | 2022 | Infectious Diseases & Immunity2022，2，1： | 0 |
| 10 | Spatial changes and driving factors of lake water quality in Inner Mongolia, China显示文摘Lakes play important roles in sustaining the ecosystem and economic development in Inner Mongolia Autonomous Region of China,but the spatial patterns and driving mechanisms of water quality in lakes so far remain unclear.This study aimed to identify the spatial changes in water quality and the driving factors of seven lakes(Juyanhai Lake,Ulansuhai Lake,Hongjiannao Lake,Daihai Lake,Chagannaoer Lake,Hulun Lake,and Wulannuoer Lake)across the longitudinal axis(from the west to the east)of Inner Mongolia.Large-scale research was conducted using the comprehensive trophic level index(TLI(Σ)),multivariate statistics,and spatial analysis methods.The results showed that most lakes in Inner Mongolia were weakly alkaline.Total dissolved solids and salinity of lake water showed obvious zonation characteristics.Nitrogen and phosphorus were identified as the main pollutants in lakes,with high average concentrations of total nitrogen and total phosphorus being of 4.05 and 0.21 mg/L,respectively.The values of TLI(Σ)ranged from 49.14 to 71.77,indicating varying degrees of lake eutrophication,and phosphorus was the main driver of lake eutrophication.The lakes of Inner Mongolia could be categorized into lakes to the west of Daihai Lake and lakes to the east of Daihai Lake in terms of salinity and TLI(Σ).The salinity levels of lakes to the west of Daihai Lake exceeded those of lakes to the east of Daihai Lake,whereas the opposite trend was observed for lake trophic level.The intensity and mode of anthropogenic activities were the driving factors of the spatial patterns of lake water quality.It is recommended to control the impact of anthropogenic activities on the water quality of lakes in Inner Mongolia to improve lake ecological environment.These findings provide a more thorough understanding of the driving mechanism of the spatial patterns of water quality in lakes of Inner Mongolia,which can be used to develop strategies for lake ecosystem protection and water resources management in this region. | REN Xiaohui YU Ruihong LIU Xinyu SUN Heyang GENG Yue QI Zhen ZHANG Zhuangzhuang LI Xiangwei WANG Jun ZHU Penghang GUO Zhiwei WANG Lixin XU Jifei | 2023 | Journal of Arid Land2023，15，2： | 0 |
| 11 | Greenhouse gas emissions from Daihai Lake,China:Should eutrophication and salinity promote carbon emission dynamics?显示文摘Greenhouse gases(GHGs)emitted or absorbed by lakes are an important component of the global carbon cycle.However,few studies have focused on the GHG dynamics of eutrophic saline lakes,thus preventing a comprehensive understanding of the carbon cycle.Here,we conducted four sampling analyses using a floating chamber in Daihai Lake,a eutrophication saline lake in Inner Mongolia Autonomous Region,China,to explore its carbon dioxide(CO_(2))and methane(CH_(4))emissions.The mean CO_(2)emission flux(FCO_(2))and CH_(4)emission flux(FCH_(4))were 17.54±14.54 mmol/m^(2)/day and 0.50±0.50 mmol/m^(2)/day,respectively.The results indicated that Daihai Lake was a source of CO_(2)and CH_(4),and GHG emissions exhibited temporal variability.The mean CO_(2)partial pressure(p CO_(2))and CH_(4)partial pressure(p CH_(4))were 561.35±109.59μatm and 17.02±13.45μatm,which were supersaturated relative to the atmosphere.The regression and correlation analysis showed that the main influencing factors of p CO_(2)were wind speed,dissolved oxygen(DO),total nitrogen(TN)and Chlorophyll a(Chl.a),whereas the main influencing factors of p CH_(4)were water temperature(WT),Chl.a,nitrate nitrogen(NO_(3)^(-)-N),TN,dissolved organic carbon(DOC)and water depth.Salinity regulated carbon mineralization and organic matter decomposition,and it was an important influencing factor of p CO_(2)and p CH_(4).Additionally,the trophic level index(TLI)significantly increased p CH_(4).Our study elucidated that salinity and eutrophication play an important role in the dynamic changes of GHG emissions.However,research on eutrophic saline lakes needs to be strengthened. | Xiangwei Li Ruihong Yu Jun Wang Heyang Sun Xinyu Liu Xiaohui Ren Shuai Zhuang Zhiwei Guo Xixi Lu | 2024 | Journal of Environmental Sciences2024，，1： | 0 |
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