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| 1 | In vitro and in vivo application of RNA interference for targeting genes involved in peritrophic matrix synthesis in a lepidopteran system显示文摘大多数昆虫的 midgut 与一个半渗透的非细胞组成的试管被衬里, peritrophic 矩阵(下午) ,几丁质和蛋白质镇静。尽管各种各样的基因编码下午蛋白质被描绘了,我们他们在下午结构和功能的角色的理解是很有限的。为获得功能的信息的一条有希望的途径是 RNA 干扰,它被用来减少使用任何一个注射予幼虫以的双 stranded RNA 或喂的特定的 mRNAs 的层次。尽管这个方法很好在 dipterans 和甲虫类之昆虫被记录,它在 lepidopterans 的成功的报告被改变。在当前的学习,编码下午蛋白质的 silencing midgut 基因(昆虫肠的粘蛋白 1 ,昆虫肠的粘蛋白 4 ,下午蛋白质 1 )并且几丁质 biosynthetic 或修改的酶(几丁质 synthase-B 和几丁质 deacetylase 1 )在 noctuid 鳞翅类, Mamestra configurata ,在 vitro 并且在 vivo 被检验。在在主要 midgut 的 vitro 研究,上皮的房间准备揭示了尖锐、快速的 silencing (由 24 h ) 为编码几丁质 deacetylase 的基因 1 并且 silencing 的更慢的率(由 72 h ) 为编码下午蛋白质 1 的基因。肠的粘蛋白是的基因编码昆虫稍微由 72 h 的 silenced,而没有 silencing 为编码几丁质 synthase-B 的基因被检测。在 vivo,实验集中了于几丁质 deacetylase 1 基因是 silenced 到在 vitro 的最大的程度。与双 stranded RNA 的出生不满一月的婴儿和第四个中间形态幼虫的连续的喂导致了几丁质 deacetylase 的 silencing 1 由 24 和 36 h 分别地。喂单个剂量到出生不满一月的婴儿也由 24 h 导致了 silencing。当前的学习证明基因编码下午蛋白质能是为 RNA 干扰由的 silenced 和轮廓条件每在 lepidopterans 喂的 os。 | Umut Toprak Doug Baldwin Martin Erlandson Cedric Gillott Stephanie Harris Dwayne D. Hegedus | 2013 | Insect Science2013，20，1： | 4 |
| 2 | Identification of the Mamestra configurata (Lepidoptera Noctuidae) peritrophic matrix proteins and enzymes involved in peritrophic matrix chitin metabolism显示文摘peritrophic 矩阵(下午) 为昆虫是必要的它由食物粒子,病原体,和毒素保护 midgut 上皮免受损坏的伤害的消化系统生理学。下午也由于它的 per os 可接近性是为新害虫控制策略的开发的一个吸引人的目标。理解 PM 怎么执行这些函数, PM 的分子的体系结构用 genomic 被检验, proteomic 在 Mamestra configurata 来临(鳞翅目:Noctuidae ) ,饰有十字架的 oilseed 的一个主要害虫在北美洲收割。PM 的液体层析双人脚踏车团 spectrometry 分析识别了是分类的 82 蛋白质:(i) peritrophins,与一个 CBDIII 领域包括一个新班;(ii ) 酶在几丁质修正(几丁质 deacetylases ) 包含了,消化(丝氨酸朊酶, aminopeptidases, carboxypeptidases,脂肪分解酵素和 α-amylase ) 或另外的反应(β-1,3-glucanase,碱的磷酸酶, dsRNase,虾红素, pantetheinase ) ;(iii ) 没有已知的 orthologs,由 polycalin, REPAT, serpin, C 类型 lectin 和 Lsti99/Lsti201 和 3 新奇蛋白质组成的一个异质的组。编码下午蛋白质的基因在 midgut 主要被表示。cDNAs 编码几丁质 synthase-2 (McCHS-2 ) , chitinase (McCHI ) ,和 β-N-acetylglucosaminidase (McNAG ) 酶,在下午几丁质新陈代谢包含了,也被识别。McCHS-2 表示对显示它为在 PM,的几丁质合成负责的 midgut 特定在 midgut 的唯一的几丁质的材料。相反,编码 chitinolytic 酶的基因在多重纸巾被表示。McCHS-2, McCHI,和 McNAG 在喂幼虫的 midgut 被表示,并且唠叨活动在 PM 是在场的。这个信息被用来产生鳞翅类的下午建筑学的一个更新的模型。 | Umut Toprak Martin Erlandson Doug Baldwin Steve Karcz Lianglu Wan Cathy Coutu Cedric Gillott Dwayne D. Hegedus | 2016 | Insect Science2016，23，5： | 4 |
| 3 | High-Resolution Peripheral Quantitative Computed Tomography for the Assessment of Bone Strength and Structure: A Review by the Canadian Bone Strength Working Group显示文摘 | Angela M. Cheung Jonathan D. Adachi David A. Hanley David L. Kendler K. Shawn Davison Robert Josse Jacques P. Brown Louis-Georges Ste-Marie Richard Kremer Marta C. Erlandson Larry Dian Andrew J. Burghardt Steven K. Boyd | 2013 | Current Osteoporosis Reports2013，，2： | 2 |
| 4 | A holistic approach for determining t he entomopat hogenic potential of Bacillus thuringiensis st rains 显示文摘 | Masson L Erlandson M Puzstai-Carey M | 1998 | Appl Environ Microbiol1998，，： | 1 |
| 5 | Gastrointestinal autonomic nerve tumors: a clinicopathological immunohistochemical and ultrastructural study of 12 cases显示文摘 | Lauwers GY Erlandson RA Casper ES | 1993 | Am J Surg Pathol1993，17，9： | 1 |
| 6 | T Alger, J Horvath et al ,Flashlamp-pumped Nd: glass amplifiers for the national ignition facility 显示文摘 | A C Erlandson | 1998 | Fusion Technology1998，34，11： | 1 |
| 7 | WAP-The wireless application protocol 显示文摘 | Erlandson C Ocklind P | 1998 | Ericsson Review1998，1998，： | 1 |
| 8 | What's that all about? Differing interpretations of conversational backchannels and questions as source of miscommunication across gender boundaries显示文摘 | MULAC A ERLANDSON K T FARRAR W J | | 0，，06： | 1 |
| 9 | A chitin deacetylase and putative insect imestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix显示文摘 | Toprak U Baldwin D Erlandson M | 2008 | Insect Mol Biol2008，17，5： | 1 |
| 10 | Primary fibrosarcoma andmalignant fibrous histiocytorm of bone-a comparative ultrastructuralstudy:evidence of aspectrum of fibroblastic diferentiation显示文摘 | Antonescu CR Erlandson RA Huvos AG | | Ultrastructural Pathology0，，： | 1 |
| 11 | A nonlinear transient CAE method for vehicle shift quality prediction 显示文摘 | Jack S PLiu Paul W Erlandson | 2006 | SAE Paper2006，，： | 1 |
| 12 | Changes in nasal epithelium,in patients with severe chronic sinusitis:a clinicopathologic and electron microscopic study显示文摘 | Al-Rawi MM Eddstein DR Erlandson RA | 1998 | Laryngoscope1998，108，12： | 1 |
| 13 | Proteomics analysis of Trichoplusia ni midgut epithelial cell brush border membrane vesicles显示文摘The insect midgut epithelium is composed of columnar, goblet, and regenerative cells. Columnar epithelial cells are the most abundant and have membrane protrusions that form the brush border membrane (BBM) on their apical side. These increase surface area available for the transport of nutrients, but also provide opportunities for interaction with xenobiotics such as pathogens, toxins and host plant allelochemicals. Recent improvements in proteomic and bioinfbrmatics tools provided an opportunity to determine the proteome of the T. ni BBM in unprecedented detail. This study reports the identification of proteins from BBM vesicles (BBMVs) using single dimension polyacrylamide gel elec? trophoresis coupled with multi-dimensional protein identification technology. More than 3000 proteins were associated with the BBMV of which 697 were predicted to possess either a signal peptide, at least one transmembrane domain or a GPI-anchor signal. Of these, bioinfbrmatics analysis and manual curation predicted that 185 may be associated with the BBMV or epithelial cell plasma membrane. These are discussed with respect to their predicted functions, namely digestion, nutrient uptake, cell signaling, development, cell-cell interactions, and other functions. We believe this to be the most detailed proteomic analysis of the lepidopteran midgut epithelium membrane to date, which will provide information to better understand the biochemical, physiological and pathological processes taking place in the larval midgut. | Muhammad Afzal Javed Cathy Coutu David A. Theilmann Martin A. Erlandson Dwayne D. Hegedus | 2019 | Insect Science2019，26，3： | 1 |
| 14 | Subclassification of gastrointestinal stromaltumors based on evaluation by electron microscopy and immunohistochemistry显示文摘 | Erlandson RA Klimastra DS Woodruff JM | 1996 | UltrostrPathol1996，20，： | 1 |
| 15 | Subclassification of gastrointestinal stromal tumors based on evolution by electron microscopy and immunohistoche mistry显示文摘 | Erlandson RA Klimstra DS Woodruff JM | 1996 | Vitrastrnct Pathol1996，20，4： | 1 |
| 16 | Coaching the alpha male显示文摘 | Ludeman K Erlandson E | 2004 | Harvand Bussines''s Review2004，82，2： | 1 |
| 17 | Neuroendocrine differentiation is a common feature of thymic carcinoma显示文摘 | Lauriola L Erlandson RA Rosai J | 1998 | Am J Surg Pathol1998，22，9： | 1 |
| 18 | Immunohisto- chemical and ultrastruetural comparative study of external lamina structure in 31 cases of cellular classical and melanotic sehwannomas 显示文摘 | Huang HY Park N Erlandson RA | 2004 | Appl Immunohistochem Mol Morphol2004，12，1： | 1 |
| 19 | Subclassification of gastrointestinal stromal tumors based on evalution by electron microscopy and immmunohistochemistry显示文摘 | Erlandson RA Klimstra DS Woodruff JM | 1996 | Ultrstruct Pathol1996，20，： | 1 |
| 20 | Subclassification of gastrointestinal stromal tumours based on evolution by electron mieroscopy and immunohistochemistry显示文摘 | Klimstra DS Woodruff JM | 1996 | Ultrastruct Pathol1996，20，3： | 1 |
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