| 1 | Exceptionally high genetic variance of the doubled haploid(DH)population of poplar显示文摘Doubled haploid(DH)plants have been widely used for breeding and biological research in crops.Pop ulus spp.have been used as model woody plant species for biological research.However,the induction of DH poplar plants is onerous,and limited biological or breeding work has been carried out on DH individuals or populations.In this study,we provide an effective protocol for poplar haploid induction based on an anther culture method.A total of 96 whole DH plant lines were obtained using an F1hybrid of Populus simonii×P.nigra as a donor tree.The phenotypes of the DH population showed exceptionally high variance when compared to those of half-sib progeny of the donor tree.Each DH line displayed distinct features compared to those of the other DH lines or the donor tree.Additionally,some excellent homozygous lines have the potential to be model plants in genetic and breeding studies. | Caixia Liu Sui Wang Yi Liu Meng Wang Erqin Fan Chen Liu Shikai Zhang Chuanping Yang Junhui Wang Heike W.Sederoff Xiangling You Vincent L.Chiang Su Chen Ronald R.Sederoff Guanzheng Qu | 2023 | Journal of Forestry Research2023，34，6： | 0 |
| 2 | Qu-2,a robust poplar suspension cell line for molecular biology显示文摘Populus spp.have long been used as model woody plant species for molecular biology research.However,tissues of poplar are often recalcitrant to experimental procedures for molecular studies.We generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba×P.berolinensis‘Yinzhong’,named Qu-2.Qu-2 cells are suitable as a model biological system for studying woody plants.Qu-2 cells have many advantages over suspension cell lines derived so far from any other woody plants.Qu-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant hormone.They show exceptionally high growth rates,reaching an approximately 150-fold increase in biomass after one week of culturing.Another important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily reactivated.Qu-2 cells are suitable for molecular manipulations such as protoplast production,transient transformation,and RNA-seq analysis.Therefore,Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research,ranging from physiology to gene function.The Qu-2 cells will be made available to the plant community for research. | Caixia Liu Kailong Li Meng Wang Erqin Fan Chuanping Yang Junhui Wang Pengyue Fu Xiaolan Ge Heike W.Sederoff Ronald RSederoff Vincent LChiang Sui Wang Guanzheng Qu | 2021 | Journal of Forestry Research2021，32，2： | 0 |
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