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| 1 | A nomogram based on lymphocyte percentage for predicting hospital mortality in exertional heatstroke patients: a 13-year retrospective study显示文摘BACKGROUND: Exertional heatstroke(EHS) is a life-threatening disease without ideal prognostic markers for predicting hospital mortality.METHODS: This is a single-center retrospective study. Clinical data from EHS patients admitted to the Intensive Care Unit(ICU) of the General Hospital of Southern Theatre Command between January 1, 2008, and December 31, 2020, were recorded and analyzed. Univariate and multivariate logistic regression were used to identify the factors for mortality. The prediction model was developed with the prognostic markers, and a nomogram was established.RESULTS: The study ultimately enrolled 156 patients, and 15(9.6%) of patients died before discharge. The lymphocyte count(Lym) and percentage(Lym%) were significantly lower in nonsurvivors(P<0.05). The univariate and multivariate logistic regression analyses indicated that Lym% at the third day of admission(Lym% D3)(OR=0.609, 95%CI: 0.454–0.816) and hematocrit(HCT)(OR=0.908, 95%CI: 0.834–0.988) were independent protective factors for hospital mortality. A nomogram incorporating Lym% D3 with HCT was developed and demonstrated good discrimination and calibration ability. The comparison between the prediction model and scoring systems revealed that the prediction model had the largest area under the curve(AUC)(0.948, 95%CI: 0.900–0.977), with 100.00% sensitivity and 83.69% specificity, and a greater clinical net benefit.CONCLUSION: Severe EHS patients had a higher risk of experiencing prolonged lymphopenia. A nomogram based on Lym% D3 and HCT was developed to facilitate early identification and timely treatment of patients with potentially unfavorable prognoses. | Jiale Yang Fanghe Gong Xuezhi Shi Fanfan Wang Jing Qian Lulu Wan Yi Chen Huaisheng Chen Huasheng Tong | 2023 | World Journal of Emergency Medicine2023，14，6： | 0 |
| 2 | Preparation, identification, and clinical application ofanti-HBs monoclonal antibody that binds both wild-typeand immune escape mutant HBsAgs显示文摘Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay(ELISA),a hybridoma cell line strain secreting anti-HBs monoclonal antibody(mAb)(defined G6 mAb)was obtained.The cells grew and secreted mAb stably.Antibody titers in the culture supernatant and ascites were 2.048�106 and 4.096�106,respectively.By applying the anti-HBs G6 mAb and horseradish peroxidase(HRP)-labeled goat anti-HBs antibody,we developed a sandwich ELISA(defined G6m ELISA)for detecting both wild-type and immune escape mutant HBsAgs(IEM HBsAg).The assay was performed to detect 17 species of genome recombinant expression HBsAg,including two wild-type species and 15 IEM HBsAg species,which varied in the“a”determinant,in a group of patients infected with hepatitis B virus(HBV).The patients previously had a lower ELISA detection signal[(absorbance of patients/absorbance of normal people(P/N):1.0–4.5)].The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125μg/L;12 of 15 IEM HBsAg species(P/N≥2.5)were positive for G6 mAb.Of the positive IEM HBsAg species,two had a low absorbance value at 450 nm(A450),one had an intermediate A450 value and nine had a high A450 value,which was 7.55%(mean),59.4%and 92.1%–109.4%of the wild-type A450 value,respectively.The two species with low A450 value and the three negative species mutated at the bases 120–124 in thefirst loop of the HBV“a”determinant.Using the G6 ELISA and two commercial ELISA kits(A and B),177 patients were tested.The G6 ELISA had a significantly higher detection rate than either commercial ELISAs(19.21%vs 14.89%and 6.21%,respectively;P<0.01,P<0.05,respectively). | Fanghe LI Chunyan ZHANG Jinghua LIU Xiaoyan ZHANG Bing YAN Bo ZHANG Yongguo HUANG Jingsong GONG Yan CHEN | 2009 | Frontiers of Medicine2009，3，3： | 0 |
| 3 | Preparation and identification of monoclonal antibodies against the adenovirus vector显示文摘Objective:To prepare and identify monoclonal antibodies(McAbs)against the capsid proteins of adenovirus vector.Methods:BALB/c mice were immunized with a mixture of the purified adenovirus vector(Adv)and Al(OH)3.McAbs were produced using cell fusion technique in a conventional way.The sensitivity and specificity of monoclonal antibodies was identified by indirect enzyme linked immunosorbent assay(ELISA),immunocytochemical staining and Western blotting. Results:Six strains of hybridoma cells(A4H11,A8C7,F1H5,G1D2,G4E3 and H2G8)that can stably secrete the IgG1 McAb against Adv were obtained.After 3 months subculture and low concentration of serum adapting culture,six strains retained their stability to secrete McAb.The ascites titers were between 1:106 and 1:108.Western blot analysis demonstrated that all the McAbs reacted with one protein(about 114 kDa)which is present in wild type 3 adenovirus(wtAd3),wild type 5 adenovirus (wtAd5),wild type 7 adenovirus(wtAd7)and adenovirus vector.Conclusion:Successfully prepared six strains of hybridoma cell secreted monoclonal antibodies against the hexon proteins of adenovirus vector,and provided the substantial foundation of preclinical research of adenovirus vectors. | Chunyan Zhang Jinsong Gong Yan Chen Fanghe Li | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，4： | 0 |
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