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| 1 | Efficient correction of hemoglobinopathy-causing mutations by homologous recombination in integration-free patient iPSCs显示文摘 | MoLi KeiichiroSuzuki JingQu PreetiSaini IlirDubova FeiYi JungminLee IgnacioSancho-Martinez Guang-HuiLiu Juan Carlos IzpisuaBelmonte | 2011 | Cell Research2011，21，12： | 11 |
| 2 | Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique显示文摘Aim: To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL^pro) and its four site-directedmutants. Methods: Based on the fluorescence resonance energy transfer (FRET)principle using 5-[(2'-aminoethyl)-amino] naphthelenesulfonic acid (EDANS) and4-[[4-(dimethylamino) phenyl] azo] benzoic acid (Dabcyl) as the energy transferpair, one fluorogenic substrate was designed for the evaluation of SARS-CoV3CL^pro proteolytic activity. Results: The kinetic parameters of the fluorogenicsubstrate have been determined as Kin=404μmol·L^-1, kcat=1.08min^-1, and kcat/Km=2.7mmol^-1·L·min^-1. SARS-CoV 3CL^pro showed substantial pH and temperature-triggered activity switches, and site-directed mutagenesis analysis of SARS-CoV3CL^pro revealed that substitutions of His^41, Cys^145, and His^163 resulted in complete loss of enzymatic activity, while replacement of Met^162 with Ala caused strongly increased activity. Conclusion: This present work has provided valuable information for understanding the catalytic mechanism of SARS-CoV 3CL^pro. This FRET-based assay might supply an ideal approach for the exploration SARS-CoV 3CL^pro putative inhibitors. | ShuaiCHEN Li-liCHEN Hai-binLUO TaoSUN,JingCHEN JingCHEN FeiYIE Jian-huaCAI Jing-kangSHEN XuSHEN Hua-liangJIANG | 2005 | Acta Pharmacologica Sinica2005，26，1： | 3 |
| 3 | Efficient XML Query and Update Processing Using A Novel Prime-Based Middle Fraction Labeling Scheme显示文摘XML data can be represented by a tree or graph and the query processing for XML data requires the structural information among nodes. Designing an efficient labeling scheme for the nodes of Order-Sensitive XML trees is one of the important methods to obtain the excellent management of XML data. Previous labeling schemes such as region and prefix often sacrifice updating performance and suffer increasing labeling space when inserting new nodes. To overcome these limitations, in this paper we propose a new labeling idea of separating structure from order. According to the proposed idea, a novel Prime-based Middle Fraction Labeling Scheme(PMFLS) is designed accordingly, in which a series of algorithms are proposed to obtain the structural relationships among nodes and to support updates. PMFLS combines the advantages of both prefix and region schemes in which the structural information and sequential information are separately expressed. PMFLS also supports Order-Sensitive updates without relabeling or recalculation, and its labeling space is stable. Experiments and analysis on several benchmarks are conducted and the results show that PMFLS is efficient in handling updates and also significantly improves the performance of the query processing with good scalability. | Zunyue Qin Yong Tang Feiyi Tang Jing Xiao Changqin Huang Hongzhi Xu | 2017 | China Communications2017，14，3： | 2 |
| 4 | Design and implementation of acceptance monitor for building intrusion tolerant systems 显示文摘 | Wang Rong Wang Feiyi Byrd G | 2003 | Software-Practice and Experience2003，17，15： | 1 |
| 5 | A review of computer graphics approaches to urban modeling from a machine learning perspective显示文摘Urban modeling facilitates the generation of virtual environments for various scenarios about cities.It requires expertise and consideration,and therefore consumes massive time and computation resources.Nevertheless,related tasks sometimes result in dissatisfaction or even failure.These challenges have received significant attention from researchers in the area of computer graphics.Meanwhile,the burgeoning development of artificial intelligence motivates people to exploit machine learning,and hence improves the conventional solutions.In this paper,we present a review of approaches to urban modeling in computer graphics using machine learning in the literature published between 2010 and 2019.This serves as an overview of the current state of research on urban modeling from a machine learning perspective. | Tian FENG Feiyi FAN Tomasz BEDNARZ | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，7： | 1 |
| 6 | Fast-response fluorescent probe with favorable water solubility for highly sensitive imaging of endogenous tyrosinase in living cells and zebrafish model显示文摘Tyrosinase(TYR) is an important polyphenolic oxidase enzyme and usually regards as a biomarker of melano ma cancer.Highly effective tracking TYR activity in vivo will help to study the mechanism of TYR in living organisms and forecasts related diseases.In this study,we present a novel TYR-activatable fluo rescent probe(CHMC-DOPA) for tracking TYR activity in vitro and in vivo.CHMC-DOPA is constructed by incorporating dopamine(DOPA) moiety into a fluorescent chloro-hydroxyl-merocyanine(CHMC)scaffold.Upon exposure to TYR,the dopamine unit in CHMC-DOPA is oxidized to a dopaquinone derivative,and an intramolecular photo-induced electron transfer(PET) process between CHMC fluorophore and o-dopaquinone will take place,the fluorescence of CHMC-DOPA is quenched rapidly.Therefore,the evaluation of TYR activity is established in terms of the relationship between fluorescence quenching efficiency and TYR activity.In our experiments,CHMC-DOPA shows various advantages,such as fast response(8 min),low concentration of TYR activation(0.5 U/mL),good water-solubility,as well as the lowest detection limit(0.003 U/mL) compared with previously reported works.Furthermore,CHMCDO PA also exhibits excellent cell membrane permeability and low cytotoxicity,which is successfully used to monitor endogenous TYR activity in living cancer cells and zebrafish models.CHMC-DOPA performs well,and we anticipate that this newly designed novel platform will provide an alternative for high effective monitoring TYR activity in biosystems. | Zheng Li Xiaofeng Xia Yu You Cuifen Lu Guichun Yang Chao Ma Junqi Nie Qi Sun Shuilin Wu Jun Ren Feiyi Wang | 2021 | Chinese Chemical Letters2021，32，5： | 1 |
| 7 | Aspects of adaptive reconfiguration in a scalable intrusion tolerant system显示文摘 | BRYANT S WANG Feiyi | 2004 | Complexity2004，9，2： | 1 |
| 8 | Design and implementation of acceptance monitor for building intrusion tolerant systems 显示文摘 | Wang Rong Wang Feiyi Gregory B | 2003 | Software - Practice and Experience2003，33，14： | 1 |
| 9 | Micro-nanofiber composite biomimetic conduits promote long-gap peripheral nerve regeneration in canine models显示文摘Peripheral nerve injuries may result in severe long-gap interruptions that are challenging to repair.Autografting is the gold standard surgical approach for repairing long-gap nerve injuries but can result in prominent donor-site complications.Instead,imitating the native neural microarchitecture using synthetic conduits is expected to offer an alternative strategy for improving nerve regeneration.Here,we designed nerve conduits composed of high-resolution anisotropic microfiber grid-cordes with randomly organized nanofiber sheaths to interrogate the positive effects of these biomimetic structures on peripheral nerve regeneration.Anisotropic microfiber-grids demonstrated the capacity to directionally guide Schwann cells and neurites.Nanofiber sheaths conveyed adequate elasticity and permeability,whilst exhibiting a barrier function against the infiltration of fibroblasts.We then used the composite nerve conduits bridge 30-mm long sciatic nerve defects in canine models.At 12 months post-implant,the morphometric and histological recovery,gait recovery,electrophysiological function,and degree of muscle atrophy were assessed.The newly regenerated nerve tissue that formed within the composite nerve conduits showed restored neurological functions that were superior compared to sheaths-only scaffolds and Neurolac nerve conduit controls.Our findings demonstrate the feasibility of using synthetic biophysical cues to effectively bridge long-gap peripheral nerve injuries and indicates the promising clinical application prospects of biomimetic composite nerve conduits. | Xianhao Dong Yueyue Yang Zheheng Bao Adam C.Midgley Feiyi Li Shuxin Dai Zhuangzhuang Yang Jin Wang Lihua Liu Wenlei Li Yayuan Zheng Siyang Liu Yang Liu Weijian Yu Jun Liu Meng Fan Meifeng Zhu Zhongyang Shen Gu Xiaosong Deling Kong | 2023 | Bioactive Materials2023，，12： | 0 |
| 10 | Columnar Liquid Crystalline Corannulenes: Synthesis, Assembly and Charge-Carrier Transport Properties显示文摘Two discotic liquid crystalline(LC)molecules consisting of a bowl-shaped corannulene core and five-fold photoactive azobenzene branches with differe nt pa raff i nk side chai ns were developed.Their self-assembly,photo-resp on sive behavior and charge-carrier tran sport properties were investigated in detail.A wider mesophase temperature range was achieved when utilizi ng Ion ger and furcal flexible side chai n substituti on.2D XRD measureme nts revealed the n-packed cora nnulen e-ce ntered lb(i.e.,with furcal side alkoxy I chains)exhibited helical columnar LC phase.This packing structure also led to a higher hole charge carrier mobility of 7.07 ×1O^(-2) cm^(2)-V^(-1)s^(-1) compared to that of the counterpart la with linear side chain substitutions. | Yang Li Zunzhi Wang Mengwei Li Feiyi Chen Yang Zhao Qi Wang Fanli Lu Yi Liu Xiang-Kui Ren Long Chen | 2021 | Chinese Journal of Chemistry2021，39，9： | 0 |
| 11 | A comparative study of seismic tomography models of Southwest China显示文摘The margin of the Tibetan Plateau of Southwest China is one of the most seismically active regions of China and is the location of the China Seismic Experimental Site(CSES).Many studies have developed seismic velocity models of Southwest China,but few have compared and evaluated these models which is important for further model improvement.Thus,we compared six published seismic shear-wave velocity models of Southwest China on absolute velocity and velocity perturbation patterns.The models are derived from different types of data(e.g.,surface waves from ambient noise and earthquakes,body-wave travel times,receiver functions) and inversion methods.We interpolated the models into a uniform horizontal grid(0.5° × 0.5°) and vertically sampled them at 5,10,20,30,40,and 60 km depths.We found significant differences between the six models.Then,we selected three of them that showed greater consistency for further comparison.Our further comparisons revealed systematic biases between models in absolute velocity that may be related to different data types.The perturbation pattern of the model is especially divergent in the shallow part,but more consistent in the deep part.We conducted synthetic and inversion tests to explore possible causes and our results imply that systematic differences between the data,differences in methods,and other factors may directly affect the model.Therefore,the Southwest China velocity model still has considerable room for improvement,and the impact of inconsistency between different data types on the model needs further research.Finally,we proposed a new reference shear-wave velocity model of Southwest China(SwCM-S1.0) based on the three selected models with high consistency.We believe that this model is a better representation of more robust features of the models that are based on different data sets. | Xuezhen Zhang Xiaodong Song Feiyi Wang | 2023 | Earthquake Science2023，36，1： | 0 |
| 12 | Current Situation and Prospect of the Application of Real-World Evidence in Health Care显示文摘Objective To summarize the application of real-world evidence(RWE)in the medical and healthcare field of various countries,including relevant policies,application scenarios and application methods.Methods Relevant policies and application scenarios were obtained by consulting the official websites and public documents of various countries’healthcare institutions.Systematic literature retrieval was adopted to search PubMed,EMBASE,Cochrane Library,CNKI,CBM and Wanfang databases,and all papers related to real-world study and application were included.Then,these papers were classified and analyzed by country and application method.Results and Conclusion The RWE was mainly applied to supporting the preliminary approval of a new drug,expanding drug indications,accelerating approval or supporting conditional marketing authorizations and drug safety evaluation,etc.The United Kingdom,the United States,Germany,the Netherlands,Italy,Sweden,and France admitted RWE,but they treated the data obtained from RWE with caution.After systematic literature retrieval,a total of 701 articles were obtained,including relevant studies from 36 countries,among which the United States published 264 in total.The most common study was about using real-world data(RWD)to calculate treatment-related costs,which had a total of 259 studies.Secondly,158 articles were used for epidemiological analysis.Then,138 articles were about establishing risk models to analyze disease risk factors.A total of 70 articles were real-world efficacy evaluation of the drug treatment schemes,54 articles were about pharmacoeconomic evaluation with RWD as parameters.A total of 29 articles used RWD to build predictive models,and 15 articles used RWD to evaluate the health-related quality of life in patients.The application of RWE has been used widely in the medical and healthcare field of various countries.The application scenarios are gradually diversified,the application methods of RWD become mature,and the evidence quality of RWE is also improved greatly. | Xiao Feiyi Zhang Fang Li Xue Dong Li | 2023 | Asian Journal of Social Pharmacy2023，18，4： | 0 |
| 13 | Beating in a dish: new hopes for cardiomyocyte regeneration显示文摘 | YingGu #x0002A FeiYi #x0002A Guang-HuiLiu Juan Carlos IzpisuaBelmonte | 2013 | Cell Research2013，23，3： | 0 |
| 14 | Genetic diversity,population structure and core collection analysis of Hunan tea plant germplasm through genotyping-by-sequencing显示文摘Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant germplasm.In order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)technology.As a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were obtained.Population structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three groups.The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to 0.14.Analysis of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea germplasms.Furthermore,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire collection.This work should be valuable for conservation and utilization of tea germplasm in Hunan。 | Feiyi Huang Jihua Duan Yu Lei Zhen Liu Yankai Kang Yi Luo Yingyu Chen Youyong Li Shuoqian Liu Saijun Li Zhonghua Liu | 2022 | Beverage Plant Research2022，2，1： | 0 |
| 15 | A deep-learning-based approach for seismic surface-wave dispersion inversion(SfNet)with application to the Chinese mainland显示文摘Surface-wave tomography is an important and widely used method for imaging the crust and upper mantle velocity structure of the Earth.In this study,we proposed a deep learning(DL)method based on convolutional neural network(CNN),named SfNet,to derive the vS model from the Rayleigh wave phase and group velocity dispersion curves.Training a network model usually requires large amount of training datasets,which is labor-intensive and expensive to acquire.Here we relied on synthetics generated automatically from various spline-based vS models instead of directly using the existing vS models of an area to build the training dataset,which enhances the generalization of the DL method.In addition,we used a random sampling strategy of the dispersion periods in the training dataset,which alleviates the problem that the real data used must be sampled strictly according to the periods of training dataset.Tests using synthetic data demonstrate that the proposed method is much faster,and the results for the vS model are more accurate and robust than those of conventional methods.We applied our method to a dataset for the Chinese mainland and obtained a new reference velocity model of the Chinese continent(ChinaVs-DL1.0),which has smaller dispersion misfits than those from the traditional method.The high accuracy and efficiency of our DL approach makes it an important method for vS model inversions from large amounts of surface-wave dispersion data. | Feiyi Wang Xiaodong Song Mengkui Li | 2023 | Earthquake Science2023，36，2： | 0 |
| 16 | BcBRC1a is a negative regulator for tillering in non-heading Chinese cabbage显示文摘Shoot branching is a decisive factor for crop yield.Molecular mechanism for regulating shoot branching(tillering)needs to be determined.Plenty of previous studies have illustrated that BRANCHED1(BRC1)is a key integrator of shoot branching regulating signals.However,BcBRC1 function in non-heading Chinese cabbage(Brassica campestris ssp.chinensis)(NHCC)remains unknown.Here,we defined two BRC1 orthologs,BcBRC1a and BcBRC1b,from NHCC and focused on the BcBRC1a gene to describe its alternative splicing characteristic and structure.BcBRC1a was expressed rhythmically and mainly in leaf axils at the'Maertou'cultivar tillering stage.BcBRC1aL encoded a nuclear location protein.Its ectopic expression caused Arabidopsis growth inhibition and silencing BcBRC1a led to increased tiller numbers in'Maertou'.Removing the shoot tips of NHCC caused axillary buds to be released from apical dominance and BcBRC1a expression down-regulation.Our research determined that BcBRC1a acts as a negative regulator for tillering in non-heading Chinese cabbage and sets the foundation for further studies. | Yan Long Tianzi Zhao Lanlan Xu Wei Zhang Feiyi Huang Jianjun Wang Xilin Hou Ying Li | 2022 | Vegetable Research2022，2，1： | 0 |
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